Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix

Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics

Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis.
Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels.
Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.
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96 WELL PCR SEGMENTED PLATE

PCR-96-SG-C 10/pk
EUR 145.00
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Polystyrene-bottomed 96-well plate (ELISA)

CT361 6
EUR 73.00

Adapter for 96 well plate

3510147 1unit
EUR 5581.00

Streptavidin coated 96-well Plate

6523-5
EUR 327.00

Black 96 Well Plate, 1EA

X025-1EA 1EA
EUR 73.00

96 well filter plate (960ul each well)

SD5006 12UNIT
EUR 96.54
  • Product category: Labware/Filtration Supplies/Filter Plates - DNA

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C 10/pk
EUR 167.00
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC 5/pk
EUR 152.00
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Clear Coated 96 Well Plate, 1EA

C107-1EA 1EA
EUR 189.00

96 well filter plate (no filter)

SD5006-EMPTY 12UNIT
EUR 87.41
  • Product category: Labware/Filtration Supplies/Filter Plates - DNA

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.

CP-AXYGEM-96-50 10/pk
EUR 457.00
Description: Crystallography Plates; Crystallography Plates - Axygen

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE

PCR-96-HS-C 10/pk
EUR 159.00
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 well DNA plate with membrane (960ul each well)

SD5007 12UNIT
EUR 242.70
  • Product category: Labware/Filtration Supplies/Filter Plates - DNA

96 well RNA plate with membrane (960ul each well)

SD5009 12UNIT
EUR 260.97
  • Product category: Labware/Filtration Supplies/Filter Plates - RNA

Equine Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Eq-48T 48T
EUR 360.00
  • Should the Equine Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-Eq-96T 96T
EUR 457.00
  • Should the Equine Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Eq-48Tests 48 Tests
EUR 358.00

Equine Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-Eq-96Tests 96 Tests
EUR 490.00

96-Well Plate PCR Products Purification Kit

BS365 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/PCR Products

96-Well Plate PCR Products Purification Kit

BS3652 2xPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/PCR Products

96-Well Plate DNA Cleanup Miniprep Kit

BS369 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 2xPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate Plasmid DNA Miniprep Kit

BS415 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 2XPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 1 plate
EUR 316.00

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 1 plate
EUR 316.00

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 1 plate
EUR 316.00

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 1 plate
EUR 316.00

Polystyrene-bottomed 96-well plate + lid (ELISPOT)

CT365 5
EUR 78.00

96-Well Plate Viral DNA Miniprep Kit

VT92032 2XPlates, 192prep
EUR 413.22
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

2 x Clear 96 well Plate, 2EA

X003-2EA 2EA
EUR 65.00

5 x Clear 96 well Plate, 5EA

X003-5EA 5EA
EUR 80.00

2 x Clear 96 well Plate, 2EA

X018-2EA 2EA
EUR 65.00

4 x Clear 96 Well Plate, 4EA

X018-4EA 4EA
EUR 87.00

Black Half Area 96 Well Plate, 1EA

X023-1EA 1EA
EUR 80.00

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 572.00
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2248.00
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 722.00
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 2886.00
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224 96 assays
EUR 722.00
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224-5 5 x 96 assays
EUR 2938.00
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Kit

STA-355 96 assays
EUR 427.00
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit

STA-355-5 5 x 96 assays
EUR 1633.00
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide

STA-356 1 slide
EUR 183.00
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide

STA-356-5 5 slides
EUR 519.00
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit

STA-416 96 assays
EUR 856.00
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit

STA-416-5 5 x 96 assays
EUR 3524.00
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED

P-DW-12-HC-S 5/pk
EUR 207.00
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)

ACT-SL-96
EUR 647.00

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 2Plates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 5Plates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96 Well Plate RNA Cleanup and Concentration Kit

BRC1251 2xPlates, 192prep
EUR 250.31
  • Product category: Molecular Biology Kits/RNA - Extraction/Clean-up

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 5XPlates, 480prep
EUR 362.98
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 2XPlates, 192prep
EUR 195.50
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 2XPlates, 192prep
EUR 195.50
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 2XPlates, 192prep
EUR 195.50
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

96-well Plasmid DNA Extraction Kit (1 plate)

FAPWE-96001 1 prep
EUR 146.00

Block for 96 well plate, WSC-2620/30

4002659 1unit
EUR 382.00
Description: Bl ock for WSC-2620 / 2630

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)

CT366 5
EUR 224.00

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 2XPlates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 5XPlates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)

EXOEL-96P-1 10 Plates
EUR 213.00

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)

CBA-081 96 assays
EUR 606.00
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-well Checkpoint Kinase Activity Assay Kit

STA-414 96 assays
EUR 757.00
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit

STA-414-5 5 x 96 assays
EUR 3060.00
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit

25-224 100 plates
EUR 299.00
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)

CBA-320 96 assays
EUR 537.00
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTA55-5P 1 PK
EUR 263.00

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTG65-5P 1 PK
EUR 263.00

Adaptor for 96-well PCR plate, accessory for AgileSealer?

APS-200-96PCR
EUR 351.00

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA

C075-1EA 1EA
EUR 178.00

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA

C140-1EA 1EA
EUR 178.00

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA

C223-1EA 1EA
EUR 178.00

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 5XPlates, 480prep
EUR 487.82
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 2XPlates, 192prep
EUR 250.31
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 2XPlates, 192prep
EUR 212.25
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 5XPlates, 480prep
EUR 364.50
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96 Well PCR Plates, Fits ABI Fast Plate Machines

GP615 25 plates
EUR 212.00

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE

432071 1/pk
EUR 345.00
Description: BioCision; BioCision CoolBox

Block for 96 deep-well plate, WSC-2620/30

4002661 1unit
EUR 382.00
Description: Bl ock for WSC-2620 / 2630

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480130 1/pk
EUR 223.00
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES

480131 1/pk
EUR 218.00
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480132 1/pk
EUR 218.00
Description: Lab Equipment; Constant Temperature Equipment

Goat anti-Mouse, Coated White 96 Well Plate, 1EA

X010-1EA 1EA
EUR 109.00

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA

X014-1EA 1EA
EUR 109.00

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA

X016-1EA 1EA
EUR 109.00

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA 2EA
EUR 94.00

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA

X061-1EA 1EA
EUR 109.00

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA

X063-1EA 1EA
EUR 109.00

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML

PCR-96-AB-C-PBC 10/pk
EUR 195.00
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL 96 assays
EUR 757.00
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN 96 assays
EUR 757.00
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 96 assays
EUR 635.00
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231 120 assays
EUR 635.00
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231-5 5 x 120 assays
EUR 2648.00
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well

1018-96 1/EA
EUR 518.00

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTAG75-5P 1 PK
EUR 286.00

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA

X011-1EA 1EA
EUR 102.00

Clear 384 Well Plate

X105-1EA 1EA
EUR 112.00

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)

B814152-0002 2XPlates, 2XPlates
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)

B814152-0005 5XPlates, 5XPlates
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA

C033-1EA 1EA
EUR 178.00

Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA

C161-1EA 1EA
EUR 178.00

DNA-BIND™ PLATE, 96 WELL, CLEAR, FLAT 1/10

2525 1/pk
EUR 137.00
Description: Genomics; Genomic Plates

96 Well 0.2ml PCR Plate, Chimney-Top 10/Bag, Sterile

PCR-960-P-CH-S 1PK, 10UNIT
EUR 92.79
  • Product category: Labware/Multiwell Plates/96-Well

FALCON® 96 WELL CLEAR ROUND BOTTOM NOT TREATED ASSAY PLATE, NONSTERILE, 5/PACK, 50/CASE

353910 5/pk
EUR 91.00
Description: Assay - DL; Assay 96 well - DL

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric

CBA-104 96 assays
EUR 635.00
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric

CBA-104-5 5 x 96 assays
EUR 2613.00
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric

CBA-105 96 assays
EUR 635.00
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric

CBA-105-5 5 x 96 assays
EUR 2613.00
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106 96 assays
EUR 635.00
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106-5 5 x 96 assays
EUR 2613.00
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-Well Phagocytosis Assay (E. coli, Colorimetric Format)

CBA-222 96 assays
EUR 693.00
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, E. coli Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-Well Phagocytosis Assay(Zymosan Substrate), Trial Size

CBA-224-T 20 assays
EUR 386.00
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

EZCellTM Cell Invasion Assay (laminin), 96-well, 8 µm

K914-100
EUR 566.00

96 WELL CLEAR PCR MICROPLATE

PCR-96-C 10/pk
EUR 145.00
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (5 x 96 assays)

CBA-320-5 5 x 96 assays
EUR 2114.00
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

Goat anti-Mouse, 1 x 8 Strip Well, Clear Coated 96 Well Plate, 1EA

X012-1EA 1EA
EUR 109.00

96-Well CometSlides

4253-096-03 1 Tests
EUR 142.40

PCR 96-Plate unskirted

A28239 25 pcs
EUR 98.00

Repeatability of a Commercially Available ELISATest for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions.
The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later.
The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at -20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing.
The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively.
Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cut-off 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK™ has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.

Individual and herd-level milk ELISA test status for Johne’s disease in Ireland after correcting for non-disease-associated variables

Antibody-detecting tests for Mycobacterium avium ssp. paratuberculosis (MAP) have low sensitivity and imperfect specificity for detection of infection. Sensitivity increases as the disease progresses. Aside from infection status and stage of disease, several factors affect test performance.
These factors have not yet been studied in dairy cows producing lower volumes of milk with higher solids concentration, such as those managed in low-input, pasture-based production systems.
Furthermore, the effect of correcting for these associations on individual and herd test status is also unknown. The first objective of this study was to examine the relationship between MAP antibody response in milk and milk yield, somatic cell count (SCC), fat and protein contents, and stage of lactation in dairy cows enrolled in the national Johne’s Disease Control Programme (JDCP) in Ireland.
The second objective was to examine the effect of correcting the antibody response for these associations on the test status of individual cows and herds, given that individual tests are often used to define a herd’s status. Data were extracted for herds in the JDCP from January 2014 to December 2015 inclusive, consisting of 42,657 milk recordings from 18,569 cows across 187 dairy herds.
Two linear regression models were constructed to investigate the association between log-transformed MAP sample-to-positive ratio and milk recording data and in primi- and multiparous cows.
Days in milk was modeled as a B-spline in each model, and cow and herd were included as random effects. Across both models, natural log-transformed MAP antibody response was negatively associated with milk yield, positively associated with protein and fat production, and had a curvilinear association with log-transformed SCC.
The association between MAP antibody response and days in milk varied over the course of the lactation. However, when combined, these variables explained only 5.1% of the variation in the antibody response of the population. After correcting for these associations, 93 multiparous cows and 20 primiparous cows changed category (negative, suspect, or positive).
When considered at the herd-test level, out of a total of 531 herd tests, 1 herd changed from negative to positive, and 5 herds changed from positive to negative. This study provides useful information to aid in the interpretation of antibody results for herds testing animals for the presence of MAP infection. At an overall population level, correction of the serological response for non-disease-associated factors has the potential to change the status of only a small number of cows.
At the herd level, the proportion of herds changing status was minimal. However, depending on the implications of a herd-level serological diagnosis, consideration should be given to correcting for these non-disease-associated variables within the context of national JD control programs.

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