Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics
Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis.
Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels.
Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.

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Black 96 Well Plate, 1EA |
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X025-1EA | Arbor Assays | 1EA | EUR 87.6 |
96 Well Semi Skirted Plate |
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PCR1216 | Scientific Laboratory Supplies | PK50 | EUR 150.48 |
96 Well Skirted PCR Plate |
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PCR1236 | Scientific Laboratory Supplies | PK50 | EUR 299.82 |
96 well PCR plate ns |
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AXY2164 | Scientific Laboratory Supplies | PK50 | EUR 220.02 |
Plate 96 Well Immulon II |
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3455 | Scientific Laboratory Supplies | PK50 | EUR 243.96 |
Streptavidin coated 96-well Plate |
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6523-5 | Biovision | each | EUR 392.4 |
96-Well Protein Folding Plate |
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PFS096 | ProFoldin | kit | EUR 347.84 |
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins. |
96 well filter plate (960ul each well) |
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SD5006 | Bio Basic | 12UNIT | EUR 115.85 |
96 Deep Well Plate Square Well V |
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PCR0246 | Scientific Laboratory Supplies | PK50 | EUR 258.78 |
Deep Well Plate 96 Well 2.0 ML |
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AXYPDW20C | Scientific Laboratory Supplies | PK50 | EUR 181.26 |
Brand 96 Well Deep Well Plate-Stackable |
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BR701354-24EA | Scientific Laboratory Supplies | PK24 | EUR 144.78 |
UNIFILTER PLATE 96 WELL 800 UL/WELL |
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WHA77002803 | Scientific Laboratory Supplies | PK25 | EUR 823.08 |
96 WELL CLEAR PCR PLATE FOR ABI |
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PCR-96-AB-C | CORNING | 10/pk | EUR 200.4 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
Equine Immunoglobulin G (IgG) ELISA Kit |
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DLR-IgG-Eq-48T | DL Develop | 48T | EUR 432 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Equine Immunoglobulin G (IgG) ELISA Kit |
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DLR-IgG-Eq-96T | DL Develop | 96T | EUR 548.4 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Equine Immunoglobulin G (IgG) ELISA Kit |
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RDR-IgG-Eq-48Tests | Reddot Biotech | 48 Tests | EUR 429.6 |
Equine Immunoglobulin G (IgG) ELISA Kit |
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RDR-IgG-Eq-96Tests | Reddot Biotech | 96 Tests | EUR 588 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
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P-DW-12-HC | CORNING | 5/pk | EUR 182.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
DEEP WELL PLATE 96 WELL 2.0 ML STERIL |
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AXYPDW20CS | Scientific Laboratory Supplies | PK50 | EUR 216.6 |
Clear Coated 96 Well Plate, 1EA |
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C107-1EA | Arbor Assays | 1EA | EUR 226.8 |
96 well filter plate (no filter) |
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SD5006-EMPTY | Bio Basic | 12UNIT | EUR 104.89 |
MULTIWELL PLATE POLYSTYRENE 96-WELL FLA |
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M2936-100EA | Scientific Laboratory Supplies | PK100 | EUR 115.14 |
PCR Plate Non Skirted 96 Well |
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MOL2364 | Scientific Laboratory Supplies | PK50 | EUR 259.69 |
PCR Plate Semi Skirted 96 Well |
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MOL2366 | Scientific Laboratory Supplies | PK50 | EUR 251.83 |
Plate 96 Well Immulon 1 FB |
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N3355 | Scientific Laboratory Supplies | PK50 | EUR 177.84 |
96 Deep Well Plate Square Wells |
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PCR0240 | Scientific Laboratory Supplies | PK100 | EUR 270.18 |
96 Deep Well Plate Round Wells |
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PCR0242 | Scientific Laboratory Supplies | PK50 | EUR 232.56 |
96 Deep Well Plate Square WellU |
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PCR0256 | Scientific Laboratory Supplies | PK50 | EUR 180.12 |
96 Well Solid Bottom Plate Black |
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PCR0354 | Scientific Laboratory Supplies | PK100 | EUR 343.14 |
96 Well Solid Bottom Plate White |
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PCR0360 | Scientific Laboratory Supplies | PK100 | EUR 313.5 |
96 Well PCR Plate Clear Wells |
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PCR0972 | Scientific Laboratory Supplies | PK50 | EUR 201.78 |
96 Well PCR Plate Divisible In12 |
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PCR1064 | Scientific Laboratory Supplies | PK50 | EUR 128.82 |
96 Well Semi-Skirted PCR Plate |
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PCR1106 | Scientific Laboratory Supplies | PK50 | EUR 285 |
S100 96-well PCR Plate Adapter |
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E5391070026 | Scientific Laboratory Supplies | EACH | EUR 335.16 |
S200 96-well PCR Plate Adapter |
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E5392070038 | Scientific Laboratory Supplies | EACH | EUR 335.16 |
96 Well ABI Fast Plate Pcr |
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PCR1200 | Scientific Laboratory Supplies | PK50 | EUR 201.78 |
96 Well Roche Style Qpcr Plate |
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PCR1210 | Scientific Laboratory Supplies | PK50 | EUR 223.44 |
96 Well Roche Style Pcr Plate |
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PCR1214 | Scientific Laboratory Supplies | PK50 | EUR 233.7 |
96 Well Roche Style PCR Plate |
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PCR1218 | Scientific Laboratory Supplies | PK50 | EUR 213.18 |
96 Well Skirted Plate+ Q-Stick |
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PCR1238 | Scientific Laboratory Supplies | PK50 | EUR 218.88 |
Random Access 96 Well Plate Skir |
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PCR1412 | Scientific Laboratory Supplies | PK50 | EUR 233.7 |
Nunc Lid for 96 well plate |
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264122 | Scientific Laboratory Supplies | PK50 | EUR 116.28 |
Falcon Microtest Black 96 Well Plate |
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353376 | Scientific Laboratory Supplies | PK32 | EUR 199.5 |
INTELLI-PLATE 96-2 Shallow well |
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MAR-102-0001-20 | MiTeGen | 120 PLATES | EUR 990 |
Description: INTELLI-PLATE 96-2 Shallow well; 2-well sitting drop, 120 plates |
24 Well Solid Bottom Assay Plate |
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PCR0352 | Scientific Laboratory Supplies | PK100 | EUR 373.92 |
Benchmark Beadblaster 96 Adapter for 96 Well Plate |
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HOM3208 | Scientific Laboratory Supplies | PK2 | EUR 1423.86 |
96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE. |
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CP-AXYGEM-96-50 | CORNING | 10/pk | EUR 548.4 |
Description: Crystallography Plates; Crystallography Plates - Axygen |
96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE |
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PCR-96-HS-C | CORNING | 10/pk | EUR 190.8 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 well DNA plate with membrane (960ul each well) |
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SD5007 | Bio Basic | 12UNIT | EUR 291.24 |
96 well RNA plate with membrane (960ul each well) |
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SD5009 | Bio Basic | 12UNIT | EUR 313.16 |
Radius 96-Well Cell Migration Assay |
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CBA-126 | Cell Biolabs | 96 assays | EUR 686.4 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
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CBA-126-5 | Cell Biolabs | 5 x 96 assays | EUR 2697.6 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
CytoSelect 96-well Cell Transformation Assay |
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CBA-130 | Cell Biolabs | 96 assays | EUR 866.4 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Cell Transformation Assay |
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CBA-130-5 | Cell Biolabs | 5 x 96 assays | EUR 3463.2 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
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CBA-224 | Cell Biolabs | 96 assays | EUR 866.4 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
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CBA-224-5 | Cell Biolabs | 5 x 96 assays | EUR 3525.6 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
OxiSelect 96-Well Comet Assay Kit |
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STA-355 | Cell Biolabs | 96 assays | EUR 512.4 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Kit |
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STA-355-5 | Cell Biolabs | 5 x 96 assays | EUR 1959.6 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Slide |
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STA-356 | Cell Biolabs | 1 slide | EUR 219.6 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
OxiSelect 96-Well Comet Assay Slide |
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STA-356-5 | Cell Biolabs | 5 slides | EUR 622.8 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
96-Well ROCK Activity Assay Kit |
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STA-416 | Cell Biolabs | 96 assays | EUR 1027.2 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
96-Well ROCK Activity Assay Kit |
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STA-416-5 | Cell Biolabs | 5 x 96 assays | EUR 4228.8 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate |
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HOM3232 | Scientific Laboratory Supplies | EACH | EUR 98.04 |
Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
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CT365 | U-CyTech | 5 | EUR 93.6 |
96 Well Breast Tumor Genomic DNA Plate |
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D8235086-1 | Biochain | 1 plate | EUR 379.2 |
96 Well Colon Tumor Genomic DNA Plate |
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D8235090-1 | Biochain | 1 plate | EUR 379.2 |
96 Well Lung Tumor Genomic DNA Plate |
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D8235152-1 | Biochain | 1 plate | EUR 379.2 |
96 Well Stomach Tumor Genomic DNA Plate |
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D8235248-1 | Biochain | 1 plate | EUR 379.2 |
96-Well Plate PCR Products Purification Kit |
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BS365 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate PCR Products Purification Kit |
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BS3652 | Bio Basic | 2xPlates, 192prep | EUR 186.19 |
96-Well Plate DNA Cleanup Miniprep Kit |
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BS369 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate DNA Cleanup Miniprep Kit |
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BS3692 | Bio Basic | 2xPlates, 192prep | EUR 186.19 |
96-Well Plate Plasmid DNA Miniprep Kit |
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BS415 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate Plasmid DNA Miniprep Kit |
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BS4152 | Bio Basic | 2XPlates, 192prep | EUR 186.19 |
96-Well Plate Viral DNA Miniprep Kit |
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VT92032 | Bio Basic | 2XPlates, 192prep | EUR 495.86 |
2 x Clear 96 well Plate, 2EA |
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X003-2EA | Arbor Assays | 2EA | EUR 78 |
5 x Clear 96 well Plate, 5EA |
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X003-5EA | Arbor Assays | 5EA | EUR 96 |
2 x Clear 96 well Plate, 2EA |
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X018-2EA | Arbor Assays | 2EA | EUR 78 |
4 x Clear 96 Well Plate, 4EA |
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X018-4EA | Arbor Assays | 4EA | EUR 104.4 |
Black Half Area 96 Well Plate, 1EA |
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X023-1EA | Arbor Assays | 1EA | EUR 96 |
PCR Plate 96 Well No Skirt Nat |
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LW2200 | Scientific Laboratory Supplies | PK100 | EUR 452.58 |
MULTIWELL PLATE, POLYSTYRENE 96-WELL U-B |
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M2186-100EA | Scientific Laboratory Supplies | PK100 | EUR 115.14 |
96 Deep Well Plate Round Wells U |
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PCR0228 | Scientific Laboratory Supplies | PK100 | EUR 166.44 |
96 Deep Well Plate Round Wells V |
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PCR0230 | Scientific Laboratory Supplies | PK100 | EUR 166.44 |
96 Well PCR Plate Divisible In 8 |
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PCR1060 | Scientific Laboratory Supplies | PK50 | EUR 128.82 |
96 Well PCR Plate Divisible In 8 |
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PCR1062 | Scientific Laboratory Supplies | PK50 | EUR 140.22 |
96-well PCR Plate Non-Skirted natural |
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AB-0600 | Scientific Laboratory Supplies | PK25 | EUR 160.74 |
96-well PCR Plate Non-Skirted (White) |
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AB-0600W | Scientific Laboratory Supplies | PK25 | EUR 191.52 |
96-well PCR Plate (Segmented Semi-Skirted) |
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AB-0900 | Scientific Laboratory Supplies | PK25 | EUR 159.6 |
SuperPlate 96-well PCR Plate Semi-Skirted |
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AB-2400 | Scientific Laboratory Supplies | PK25 | EUR 207.48 |
Thermo Fast 96 Well PCR Plate - PK25 |
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AB0800 | Scientific Laboratory Supplies | PK25 | EUR 225.72 |
Nunc Lid for 96 Well Plate SH |
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263339 | Scientific Laboratory Supplies | PK100 | EUR 102.6 |
96 Well Impact MicroBatch Plate, 80/CSE |
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M-673101 | MiTeGen | 80 PLATES | EUR 808 |
Description: 96 Well Impact MicroBatch Plate, 80/CSE |
SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included) |
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ACT-SL-96 | ACTGene | each | EUR 776.4 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
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P-DW-12-HC-S | CORNING | 5/pk | EUR 248.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
Benchmark Beadblaster 96 96 Well Plate Prefilled with 4mm Beads |
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HOM3228 | Scientific Laboratory Supplies | PK10 | EUR 2199.06 |
96 WELL PLATE; SQUARE; U BASE; 1.6ML; PP; 96/PK |
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60180-P155 | Scientific Laboratory Supplies | PK96 | EUR 484.5 |
CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric) |
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CBA-081 | Cell Biolabs | 96 assays | EUR 727.2 |
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included. |
96-well Checkpoint Kinase Activity Assay Kit |
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STA-414 | Cell Biolabs | 96 assays | EUR 908.4 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-well Checkpoint Kinase Activity Assay Kit |
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STA-414-5 | Cell Biolabs | 5 x 96 assays | EUR 3672 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
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CT366 | U-CyTech | 5 | EUR 268.8 |
96 Well Plate RNA Cleanup and Concentration Kit |
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BRC1251 | Bio Basic | 2xPlates, 192prep | EUR 300.37 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS437 | Bio Basic | 5XPlates, 480prep | EUR 435.58 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS4372 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
96 Well Plate Yeast Genomic DNA Miniprep Kit |
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BS8357 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
96-Well Plate Plant Genomic DNA Miniprep Kit |
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BS8361 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92031 | Bio Basic | 2Plates, 192prep | EUR 210.85 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92032 | Bio Basic | 5Plates, 480prep | EUR 389.9 |
96-well Plasmid DNA Extraction Kit (1 plate) |
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FAPWE-96001 | Favorgen | 1 prep | EUR 175.2 |
Block for 96 well plate, WSC-2620/30 |
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4002659 | Atto | 1unit | EUR 458.4 |
Description: Bl ock for WSC-2620 / 2630 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1292 | Bio Basic | 2XPlates, 192prep | EUR 210.85 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1295 | Bio Basic | 5XPlates, 480prep | EUR 389.9 |
Adapter for 96-well PCR plate 2 pcs. |
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E5825711009 | Scientific Laboratory Supplies | PK2 | EUR 150.48 |
96 WELL PLATE; SQUARE; 2ML; PP; 1/PK |
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60180-P403 | Scientific Laboratory Supplies | EACH | EUR 17.1 |
96-well PCR Plate (Non-Skirted+black lettering) |
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AB-0600-L | Scientific Laboratory Supplies | PK25 | EUR 168.72 |
96-well Low Profile PCR Plate Non-Skirted |
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AB-0700 | Scientific Laboratory Supplies | PK25 | EUR 171 |
Abgene Thermo 96 Well PCR Plate Skirted Blue |
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AB0800B | Scientific Laboratory Supplies | PK25 | EUR 245.1 |
Culture Plate 96 Well Non Treated Ind. Wrapped |
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ARG1476 | Scientific Laboratory Supplies | PK100 | EUR 302.1 |
Tissue Culture Plate 96 Well Treated Ind. Wrapped |
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ARG1492 | Scientific Laboratory Supplies | PK100 | EUR 362.52 |
Microbiol clear skirted PCR plate 96 well 10pcs |
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E0030129300 | Scientific Laboratory Supplies | EACH | EUR 82.08 |
Repeatability of a Commercially Available ELISATest for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk
An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions.
The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later.
The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at -20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing.
The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively.
Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cut-off 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK™ has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.
Individual and herd-level milk ELISA test status for Johne’s disease in Ireland after correcting for non-disease-associated variables
Antibody-detecting tests for Mycobacterium avium ssp. paratuberculosis (MAP) have low sensitivity and imperfect specificity for detection of infection. Sensitivity increases as the disease progresses. Aside from infection status and stage of disease, several factors affect test performance.
These factors have not yet been studied in dairy cows producing lower volumes of milk with higher solids concentration, such as those managed in low-input, pasture-based production systems.
Furthermore, the effect of correcting for these associations on individual and herd test status is also unknown. The first objective of this study was to examine the relationship between MAP antibody response in milk and milk yield, somatic cell count (SCC), fat and protein contents, and stage of lactation in dairy cows enrolled in the national Johne’s Disease Control Programme (JDCP) in Ireland.
The second objective was to examine the effect of correcting the antibody response for these associations on the test status of individual cows and herds, given that individual tests are often used to define a herd’s status. Data were extracted for herds in the JDCP from January 2014 to December 2015 inclusive, consisting of 42,657 milk recordings from 18,569 cows across 187 dairy herds.
Two linear regression models were constructed to investigate the association between log-transformed MAP sample-to-positive ratio and milk recording data and in primi- and multiparous cows.
Days in milk was modeled as a B-spline in each model, and cow and herd were included as random effects. Across both models, natural log-transformed MAP antibody response was negatively associated with milk yield, positively associated with protein and fat production, and had a curvilinear association with log-transformed SCC.
The association between MAP antibody response and days in milk varied over the course of the lactation. However, when combined, these variables explained only 5.1% of the variation in the antibody response of the population. After correcting for these associations, 93 multiparous cows and 20 primiparous cows changed category (negative, suspect, or positive).
When considered at the herd-test level, out of a total of 531 herd tests, 1 herd changed from negative to positive, and 5 herds changed from positive to negative. This study provides useful information to aid in the interpretation of antibody results for herds testing animals for the presence of MAP infection. At an overall population level, correction of the serological response for non-disease-associated factors has the potential to change the status of only a small number of cows.
At the herd level, the proportion of herds changing status was minimal. However, depending on the implications of a herd-level serological diagnosis, consideration should be given to correcting for these non-disease-associated variables within the context of national JD control programs.
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS700A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS720A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS740A-KIT | SBI | 10 rxn | EUR 1358.4 |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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CAS510A-KIT | SBI | 1 Kit | EUR 966 |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV200PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV205PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV220PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV225PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS750A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS770A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|||
CAS790A-KIT | SBI | 10 rxn | EUR 1358.4 |
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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CAS9LIG-KIT | SBI | 1 Kit | EUR 183.6 |
Adiponectin (human) Elisa Assay Kit |
|||
K2192-96 | ApexBio | 96 assays | EUR 1220.4 |
Adiponectin (mouse) Elisa Assay Kit |
|||
K2193-96 | ApexBio | 96 assays | EUR 1220.4 |
Adiponectin (human) ELISA Assay Kit |
|||
K4901-100 | Biovision | each | EUR 1077.6 |
Adiponectin (mouse) Elisa Assay Kit |
|||
K4902-100 | Biovision | each | EUR 1038 |
Adiponectin (rat) Elisa Assay Kit |
|||
K4903-100 | Biovision | each | EUR 1011.6 |
Visfatin (human) Elisa Assay Kit |
|||
K4907-100 | Biovision | each | EUR 1182 |
Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
|||
SPXMT-KIT | Scientific Laboratory Supplies | 30ML | EUR 237.12 |
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|||
PIN320A-KIT | SBI | 1 Kit | EUR 5929.2 |
Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
|||
SPXHM-KIT | Scientific Laboratory Supplies | 30ML | EUR 245.1 |
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|||
PIN340iPS-KIT | SBI | 1 Kit | EUR 5929.2 |
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
GE620A-KIT | SBI | 1 kit | EUR 2558.4 |
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
GE622A-KIT | SBI | 1 kit | EUR 2558.4 |
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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GE624A-KIT | SBI | 1 kit | EUR 2558.4 |
Proteasome Assay Kit |
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55R-1341 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Proteasome in the research laboratory |
Calpain Assay Kit |
|||
55R-1342 | Fitzgerald | 100 assays | EUR 934.8 |
Description: Assay Kit for detection of Calpain in the research laboratory |
Glutathione Assay Kit |
|||
55R-1343 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Glutathione in the research laboratory |
Glutathione Assay Kit |
|||
55R-1354 | Fitzgerald | 100 assays | EUR 1074 |
Description: Assay Kit for detection of Glutathione activity in the research laboratory |
HDAC Assay Kit |
|||
55R-1371 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of HDAC in the research laboratory |
HDAC Assay Kit |
|||
55R-1372 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of HDAC in the research laboratory |
HAT Assay Kit |
|||
55R-1373 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of HAT in the research laboratory |
SOD Assay Kit |
|||
55R-1374 | Fitzgerald | 100 assays | EUR 722.4 |
Description: Assay Kit for detection of SOD in the research laboratory |
HDAC3 Assay Kit |
|||
55R-1377 | Fitzgerald | 100 assays | EUR 831.6 |
Description: Assay Kit for detection of HDAC3 in the research laboratory |
HDAC8 Assay Kit |
|||
55R-1379 | Fitzgerald | 100 assays | EUR 826.8 |
Description: Assay Kit for detection of HDAC8 in the research laboratory |
ATP Assay Kit |
|||
55R-1380 | Fitzgerald | 100 assays | EUR 970.8 |
Description: Assay Kit for detection of ATP in the research laboratory |
ADP Assay Kit |
|||
55R-1381 | Fitzgerald | 100 assays | EUR 970.8 |
Description: Assay Kit for detection of ADP in the research laboratory |
FAD Assay Kit |
|||
55R-1382 | Fitzgerald | 100 assays | EUR 878.4 |
Description: Assay Kit for detection of FAD in the research laboratory |
PEP Assay Kit |
|||
55R-1384 | Fitzgerald | 100 assays | EUR 1051.2 |
Description: Assay Kit for detection of PEP in the research laboratory |
Ammonia Assay Kit |
|||
55R-1388 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Ammonia in the research laboratory |
cAMP Assay Kit |
|||
55R-1389 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of cAMP activity in the research laboratory |
cGMP Assay Kit |
|||
55R-1390 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of cGMP activity in the research laboratory |
Urea Assay Kit |
|||
55R-1391 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Urea in the research laboratory |
Calcium Assay Kit |
|||
55R-1392 | Fitzgerald | 250 assays | EUR 703.2 |
Description: Assay Kit for detection of Calcium activity in the research laboratory |
Magnesium Assay Kit |
|||
55R-1393 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Magnesium in the research laboratory |
Zinc Assay Kit |
|||
55R-1394 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of Zinc in the research laboratory |
Iron Assay Kit |
|||
55R-1395 | Fitzgerald | 100 assays | EUR 909.6 |
Description: Assay Kit for detection of Iron in the research laboratory |
Phosphate Assay Kit |
|||
55R-1400 | Fitzgerald | 500 assays | EUR 498 |
Description: Assay Kit for detection of Phosphate in the research laboratory |
JNK Assay Kit |
|||
55R-1407 | Fitzgerald | 40 assays | EUR 1041.6 |
Description: Assay Kit for detection of JNK in the research laboratory |
JNK Assay Kit |
|||
55R-1408 | Fitzgerald | 40 assays | EUR 1174.8 |
Description: Assay Kit for detection of JNK in the research laboratory |
Akt Assay Kit |
|||
55R-1409 | Fitzgerald | 40 assays | EUR 1174.8 |
Description: Assay Kit for detection of Akt in the research laboratory |
Ammonia Assay Kit |
|||
55R-1410 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Ammonia in the research laboratory |
Cobalt Assay Kit |
|||
55R-1435 | Fitzgerald | 100 assays | EUR 745.2 |
Description: Assay Kit for detection of Cobalt in the research laboratory |
Nickel Assay Kit |
|||
55R-1436 | Fitzgerald | 100 assays | EUR 745.2 |
Description: Assay Kit for detection of Nickel in the research laboratory |
Chloride Assay Kit |
|||
55R-1437 | Fitzgerald | 100 assays | EUR 580.8 |
Description: Assay Kit for detection of Chloride in the research laboratory |
Aspartate Assay Kit |
|||
55R-1438 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of Aspartate in the research laboratory |
Hydroxyproline Assay Kit |
|||
55R-1439 | Fitzgerald | 100 assays | EUR 1012.8 |
Description: Assay Kit for detection of Hydroxyproline in the research laboratory |
Phenylalanine Assay Kit |
|||
55R-1442 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Phenylalanine in the research laboratory |
Phosphatidylcholine Assay Kit |
|||
55R-1443 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory |
Glucose Assay Kit |
|||
55R-1449 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of Glucose in the research laboratory |
Lactate Assay Kit |
|||
55R-1450 | Fitzgerald | 100 assays | EUR 1012.8 |
Description: Assay Kit for detection of Lactate in the research laboratory |
Pyruvate Assay Kit |
|||
55R-1452 | Fitzgerald | 100 assays | EUR 1012.8 |
Description: Assay Kit for detection of Pyruvate in the research laboratory |
Adipogenesis Assay Kit |
|||
55R-1454 | Fitzgerald | 100 assays | EUR 703.2 |
Description: Assay Kit for detection of Adipogenesis in the research laboratory |
Glucose Assay Kit |
|||
55R-1458 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Glucose in the research laboratory |
Galactose Assay Kit |
|||
55R-1459 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Galactose in the research laboratory |
Maltose Assay Kit |
|||
55R-1460 | Fitzgerald | 100 assays | EUR 867.6 |
Description: Assay Kit for detection of Maltose in the research laboratory |
Fructose Assay Kit |
|||
55R-1461 | Fitzgerald | 100 assays | EUR 826.8 |
Description: Assay Kit for detection of Fructose in the research laboratory |
Ethanol Assay Kit |
|||
55R-1462 | Fitzgerald | 100 assays | EUR 928.8 |
Description: Assay Kit for detection of Ethanol in the research laboratory |
Galactose Assay Kit |
|||
55R-1463 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of Galactose in the research laboratory |
Lactose Assay Kit |
|||
55R-1466 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of Lactose in the research laboratory |
Creatinine Assay Kit |
|||
55R-1467 | Fitzgerald | 100 assays | EUR 765.6 |
Description: Assay Kit for detection of Creatinine in the research laboratory |
Sucrose Assay Kit |
|||
55R-1468 | Fitzgerald | 100 assays | EUR 826.8 |
Description: Assay Kit for detection of Sucrose in the research laboratory |
Lactate Assay Kit |
|||
55R-1469 | Fitzgerald | 100 assays | EUR 1012.8 |
Description: Assay Kit for detection of Lactate in the research laboratory |
Maltose Assay Kit |
|||
55R-1470 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of Maltose in the research laboratory |
Glutamate Assay Kit |
|||
55R-1471 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Glutamate in the research laboratory |
Fumarate Assay Kit |
|||
55R-1475 | Fitzgerald | 100 assays | EUR 847.2 |
Description: Assay Kit for detection of Fumarate in the research laboratory |
Creatine Assay Kit |
|||
55R-1476 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Creatine in the research laboratory |
Sarcosine Assay Kit |
|||
55R-1477 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Sarcosine in the research laboratory |
Malate Assay Kit |
|||
55R-1478 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of Malate in the research laboratory |
Glycogen Assay Kit |
|||
55R-1481 | Fitzgerald | 100 assays | EUR 1012.8 |
Description: Assay Kit for detection of Glycogen in the research laboratory |
Starch Assay Kit |
|||
55R-1482 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of Starch in the research laboratory |
Alanine Assay Kit |
|||
55R-1483 | Fitzgerald | 100 assays | EUR 784.8 |
Description: Assay Kit for detection of Alanine in the research laboratory |
Formate Assay Kit |
|||
55R-1484 | Fitzgerald | 100 assays | EUR 970.8 |
Description: Assay Kit for detection of Formate in the research laboratory |
Citrate Assay Kit |
|||
55R-1485 | Fitzgerald | 100 assays | EUR 888 |
Description: Assay Kit for detection of Citrate in the research laboratory |
Isocitrate Assay Kit |
|||
55R-1486 | Fitzgerald | 100 assays | EUR 795.6 |
Description: Assay Kit for detection of Isocitrate in the research laboratory |
Oxaloacetate Assay Kit |
|||
55R-1488 | Fitzgerald | 100 assays | EUR 795.6 |
Description: Assay Kit for detection of Oxaloacetate in the research laboratory |
Heme Assay Kit |
|||
55R-1492 | Fitzgerald | 100 assays | EUR 807.6 |
Description: Assay Kit for detection of Heme in the research laboratory |