Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix

September 8, 2020 Leave a comment

Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics

Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis.
Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels.
Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.
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96-well Array Plate
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EUR 232
Description: Chromatography Reagents

AssayStar 384 Square Well Assay Plate, Black Solid Bottom - 100 plates
WB2-4250 each Ask for price

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96 well filter plate (960ul each well)
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Black 96 Well Plate, 1EA
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NanoECM®; 96 Well Plate
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NanoAligned®; 96 Well Plate
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IL4 ELISA Kit (Equine) : 96 Wells (OKEH00132)
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Aligned Nanofibers 96 Well Plate
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Streptavidin coated 96-well Plate
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Deep Well Plate 96 Well 2.0 ML - PK50
AXYPDW20C PK50
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96 Deep Well Plate Square Well V - PK50
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96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well,
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UNIFILTER PLATE 96 WELL 800 UL/WELL - PK25
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1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
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Cell Culture Plate, 96 Well
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96 WELL PCR SEGMENTED PLATE
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Tissue Culture Plate, 96 Well
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Brand 96 Well Deep Well Plate-Stackable - PK24
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96-Well EIA Microtiter Plate
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96-Well EIA Microtiter Plate
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Clear Coated 96 Well Plate, 1EA
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96-Well Protein Folding Plate
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96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well,
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96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well,
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96-well Black Polystyrene Plate
MBS258058-1Plate 1Plate
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96-well Black Polystyrene Plate
MBS258058-5x1Plate 5x1Plate
EUR 340

Nori Equine IgG1 ELISA Kit (2 plates)
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BioMag® 96-Well Plate Separator
8MB4109S-1 1magnet
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DEEP WELL PLATE 96 WELL 2.0 ML STERIL - PK50
AXYPDW20CS PK50
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96 well PCR plate ns - PK50
AXY2164 PK50
EUR 268.65

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
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2 x Clear 96 well Plate, 2EA
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5 x Clear 96 well Plate, 5EA
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2 x Clear 96 well Plate, 2EA
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4 x Clear 96 Well Plate, 4EA
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Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
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Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
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96 Deep Well Plate Round Wells - PK50
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Plate 96 Well Immulon II - PK50
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96 Well Roche Style PCR Plate
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96 Deep Well Plate Square WellU - PK50
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96 Well Skirted PCR Plate - PK50
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96 well filter plate (no filter)
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Nunc Lid for 96 well plate - PK50
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96 Deep Well Plate Square Wells - PK100
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96 Well Semi Skirted Plate - PK50
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Black Half Area 96 Well Plate, 1EA
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96 well DNA plate with membrane (960ul each well)
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96 well RNA plate with membrane (960ul each well)
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96-well plate Plasmid DNA Prep (mini)
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96 Well PCR Plate Clear Wells - PK50
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Random Access 96 Well Plate Skir - PK50
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96-Well Plate Viral DNA Miniprep Kit
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96-Well Plate Plasmid DNA Miniprep Kit
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Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
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96-Well Plate DNA Cleanup Miniprep Kit
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Falcon Microtest Black 96 Well Plate - PK32
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DNA Coating Solution, with 96-well Plate
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PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
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ELISA Flex: Equine IgG (ALP)
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96-Well Plate PCR Products Purification Kit
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96 WELL CLEAR PCR PLATE FOR ABI
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96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
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Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate - EACH
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Plate 96 Well Immulon 1 FB - PK50
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CORNING® BIOCOAT™ GELATIN 96 WELL CLEAR FLAT BOTTOM ASSAY PLATE, WITH LID, NONSTERILE, 5/PACK, 50/CASE
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96 Well Skirted Plate+ Q-Stick - PK50
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96-Well ROCK Activity Assay Kit
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EUR 78.5

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96-Well G-25 Gel Filtration Plate
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96-Well G-25 Gel Filtration Plate
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96-Well G-25 Gel Filtration Plate
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96-Well G-50 Gel Filtration Plate
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96-Well G-50 Gel Filtration Plate
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96-Well G-50 Gel Filtration Plate
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EUR 1382

96 Well Colon Tumor Genomic DNA Plate
D8235090-1 1 plate
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S100 96-well PCR Plate Adapter - EACH
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S200 96-well PCR Plate Adapter - EACH
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EUR 426.6

96 Well PCR Plate Divisible In12 - PK50
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EUR 195.75

96 Well Semi-Skirted PCR Plate - PK50
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EUR 414.45

Presto™ Plasmid 96 Well Binding Plate
96PBP005 each
EUR 78.5

Presto™ Plasmid 96 Well Binding Plate
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96 Well Breast Tumor Genomic DNA Plate
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96 Well Impact MicroBatch Plate, 80/CSE
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EUR 808
Description: 96 Well Impact MicroBatch Plate, 80/CSE

96 Well Solid Bottom Plate Black - PK100
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EUR 475.2

96 Well Solid Bottom Plate White - PK100
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EUR 437.4

Repeatability of a Commercially Available ELISATest for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions.
The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later.
The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at -20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing.
The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively.
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Individual and herd-level milk ELISA test status for Johne’s disease in Ireland after correcting for non-disease-associated variables

Antibody-detecting tests for Mycobacterium avium ssp. paratuberculosis (MAP) have low sensitivity and imperfect specificity for detection of infection. Sensitivity increases as the disease progresses. Aside from infection status and stage of disease, several factors affect test performance.
These factors have not yet been studied in dairy cows producing lower volumes of milk with higher solids concentration, such as those managed in low-input, pasture-based production systems.
Furthermore, the effect of correcting for these associations on individual and herd test status is also unknown. The first objective of this study was to examine the relationship between MAP antibody response in milk and milk yield, somatic cell count (SCC), fat and protein contents, and stage of lactation in dairy cows enrolled in the national Johne’s Disease Control Programme (JDCP) in Ireland.
The second objective was to examine the effect of correcting the antibody response for these associations on the test status of individual cows and herds, given that individual tests are often used to define a herd’s status. Data were extracted for herds in the JDCP from January 2014 to December 2015 inclusive, consisting of 42,657 milk recordings from 18,569 cows across 187 dairy herds.
Two linear regression models were constructed to investigate the association between log-transformed MAP sample-to-positive ratio and milk recording data and in primi- and multiparous cows.
Days in milk was modeled as a B-spline in each model, and cow and herd were included as random effects. Across both models, natural log-transformed MAP antibody response was negatively associated with milk yield, positively associated with protein and fat production, and had a curvilinear association with log-transformed SCC.
The association between MAP antibody response and days in milk varied over the course of the lactation. However, when combined, these variables explained only 5.1% of the variation in the antibody response of the population. After correcting for these associations, 93 multiparous cows and 20 primiparous cows changed category (negative, suspect, or positive).
When considered at the herd-test level, out of a total of 531 herd tests, 1 herd changed from negative to positive, and 5 herds changed from positive to negative. This study provides useful information to aid in the interpretation of antibody results for herds testing animals for the presence of MAP infection. At an overall population level, correction of the serological response for non-disease-associated factors has the potential to change the status of only a small number of cows.
At the herd level, the proportion of herds changing status was minimal. However, depending on the implications of a herd-level serological diagnosis, consideration should be given to correcting for these non-disease-associated variables within the context of national JD control programs.

Elite cAMP ELISA Assay Kit (Fluorescence)
MBS433536-1Plate 1Plate
EUR 605

Elite cAMP ELISA Assay Kit (Fluorescence)
MBS433536-5x1Plate 5x1Plate
EUR 2765

Ultra Nuclease Assay Kit 2.0(ELISA)
HBP000116 96 Tests
EUR 609.5

Cas9 ELISA Assay
MBS412289-5x96Wells 5x96Wells
EUR 2945

Cas9 ELISA Assay
MBS412289-96Wells 96Wells
EUR 640

K2/Spice Synthetic Cannabinoids ELISA Assay Kit
MBS376013-5x96Wells 5x96Wells
EUR 1145

OVALBUMIN SPECIFIC IgE ELISA ASSAY KIT (OKSA11256)
OKSA11256 96 Tests
EUR 4191.6
Description: Description of target: MOUSE OVALBUMIN SPECIFIC IgE ELISA ASSAY KIT;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity:

Screen Questâ„¢ Colorimetric ELISA cAMP Assay Kit
36370-1plate 1 plate
EUR 334
Description: Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction.

Screen Questâ„¢ Colorimetric ELISA cAMP Assay Kit
36371-10plates 10 plates
EUR 2197
Description: Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction.

Screen Questâ„¢ Fluorimetric ELISA cAMP Assay Kit
36373-1plate 1 plate
EUR 334
Description: Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems.

Screen Questâ„¢ Fluorimetric ELISA cAMP Assay Kit
36374-10plates 10 plates
EUR 2197
Description: Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems.

Assay kit
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EUR 468

Elite cAMP 384-Well ELISA Assay Kit (Fluorescence)
MBS433543-1Plate 1Plate
EUR 845

Elite cAMP 384-Well ELISA Assay Kit (Fluorescence)
MBS433543-5x1Plate 5x1Plate
EUR 3900

Elite gp120 (HIV-1/Clade B) ELISA Assay Kit
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EUR 730

Elite gp120 (HIV-1/Clade B) ELISA Assay Kit
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Rat uPA total antigen assay ELISA Kit
MBS135495-1Kit 1Kit
EUR 750

Rat uPA total antigen assay ELISA Kit
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Rat tPA total antigen assay ELISA Kit
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Rat tPA total antigen assay ELISA Kit
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ELISA Assay Buffer, 20x
GR103001 1 L
EUR 89

ELISA Assay Buffer, 80x
GR103002 1 L
EUR 159

Mouse uPA total antigen assay ELISA Kit
MBS135504-1Kit 1Kit
EUR 750

Mouse uPA total antigen assay ELISA Kit
MBS135504-5x1Kit 5x1Kit
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Human tPA total antigen assay ELISA Kit
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Human tPA total antigen assay ELISA Kit
MBS135510-5x1Kit 5x1Kit
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Human uPA total antigen assay ELISA Kit
MBS135519-1Kit 1Kit
EUR 750

Human uPA total antigen assay ELISA Kit
MBS135519-5x1Kit 5x1Kit
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Mouse tPA total antigen assay ELISA Kit
MBS135528-1Kit 1Kit
EUR 750

Mouse tPA total antigen assay ELISA Kit
MBS135528-5x1Kit 5x1Kit
EUR 3420

Active rat tPA functional assay ELISA Kit
MBS135493-1Kit 1Kit
EUR 750

Active rat tPA functional assay ELISA Kit
MBS135493-5x1Kit 5x1Kit
EUR 3420

Active rat uPA functional assay ELISA Kit
MBS135544-1Kit 1Kit
EUR 750

Active rat uPA functional assay ELISA Kit
MBS135544-5x1Kit 5x1Kit
EUR 3420

Creatinine Assay Kit (urine normalizing assay)
MBS480387-1Kit 1Kit
EUR 220

Creatinine Assay Kit (urine normalizing assay)
MBS480387-5x1Kit 5x1Kit
EUR 960

Rat Fibrinogen ELISA assay
MBS480412-1Kit 1Kit
EUR 625

Rat Fibrinogen ELISA assay
MBS480412-5x1Kit 5x1Kit
EUR 2825

Active human uPA functional assay ELISA Kit
MBS135509-1Kit 1Kit
EUR 750

Active human uPA functional assay ELISA Kit
MBS135509-5x1Kit 5x1Kit
EUR 3420

Active mouse uPA functional assay ELISA Kit
MBS135514-1Kit 1Kit
EUR 750

Active mouse uPA functional assay ELISA Kit
MBS135514-5x1Kit 5x1Kit
EUR 3420

Active human tPA functional assay ELISA Kit
MBS135524-1Kit 1Kit
EUR 750

Active human tPA functional assay ELISA Kit
MBS135524-5x1Kit 5x1Kit
EUR 3420

Active mouse tPA functional assay ELISA Kit
MBS135539-1Kit 1Kit
EUR 750

Active mouse tPA functional assay ELISA Kit
MBS135539-5x1Kit 5x1Kit
EUR 3420

Hemagglutinin (H1N1)(A/California/07/09) ELISA Assay Kit
MBS433548-200Assays 200Assays
EUR 730

Hemagglutinin (H1N1)(A/California/07/09) ELISA Assay Kit
MBS433548-5x200Assays 5x200Assays
EUR 3355

Mouse Fibrinogen ELISA assay
MBS480411-1Kit 1Kit
EUR 665

Mouse Fibrinogen ELISA assay
MBS480411-5x1Kit 5x1Kit
EUR 3000

Active rabbit tPA functional assay ELISA Kit
MBS135502-1Kit 1Kit
EUR 750

Active rabbit tPA functional assay ELISA Kit
MBS135502-5x1Kit 5x1Kit
EUR 3420

Rat fibrinogen total antigen assay ELISA Kit
MBS135508-1Kit 1Kit
EUR 490

Rat fibrinogen total antigen assay ELISA Kit
MBS135508-5x1Kit 5x1Kit
EUR 2220

Active porcine tPA functional assay ELISA Kit
MBS135507-1Kit 1Kit
EUR 750

Active porcine tPA functional assay ELISA Kit
MBS135507-5x1Kit 5x1Kit
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Human Kininogen total antigen assay ELISA kit
MBS136014-1Kit 1Kit
EUR 580

Human Kininogen total antigen assay ELISA kit
MBS136014-5x1Kit 5x1Kit
EUR 2635

JWH-250 (K2/Spice) Synthetic Cannabinoid ELISA Assay Kit
MBS376015-5x96Wells 5x96Wells
EUR 1145

Mouse fibrinogen total antigen assay ELISA Kit
MBS135511-1Kit 1Kit
EUR 490

Mouse fibrinogen total antigen assay ELISA Kit
MBS135511-5x1Kit 5x1Kit
EUR 2220

Human fibrinogen total antigen assay ELISA Kit
MBS135523-1Kit 1Kit
EUR 475

Human fibrinogen total antigen assay ELISA Kit
MBS135523-5x1Kit 5x1Kit
EUR 2120

Rat PAI-1 total antigen assay ELISA Kit
MBS135541-1Kit 1Kit
EUR 725

Rat PAI-1 total antigen assay ELISA Kit
MBS135541-5x1Kit 5x1Kit
EUR 3300

Human antiplasmin total antigen assay ELISA kit
MBS135932-1Kit 1Kit
EUR 750

Human antiplasmin total antigen assay ELISA kit
MBS135932-5x1Kit 5x1Kit
EUR 3420

Mouse antiplasmin total antigen assay ELISA kit
MBS135940-1Kit 1Kit
EUR 750

Mouse antiplasmin total antigen assay ELISA kit
MBS135940-5x1Kit 5x1Kit
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Human plasminogen total antigen assay ELISA Kit
MBS135487-1Kit 1Kit
EUR 490

Human plasminogen total antigen assay ELISA Kit
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EUR 2220

Mouse plasminogen total antigen assay ELISA Kit
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EUR 580

Mouse plasminogen total antigen assay ELISA Kit
MBS135494-5x1Kit 5x1Kit
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Human Prothrombin total antigen assay ELISA Kit
MBS135499-1Kit 1Kit
EUR 490

Human Prothrombin total antigen assay ELISA Kit
MBS135499-5x1Kit 5x1Kit
EUR 2220

Rabbit fibrinogen total antigen assay ELISA Kit
MBS135501-1Kit 1Kit
EUR 750

Rabbit fibrinogen total antigen assay ELISA Kit
MBS135501-5x1Kit 5x1Kit
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Mouse Prothrombin total antigen assay ELISA Kit
MBS135518-1Kit 1Kit
EUR 520

Mouse Prothrombin total antigen assay ELISA Kit
MBS135518-5x1Kit 5x1Kit
EUR 2340

Human vitronectin total antigen assay ELISA Kit
MBS135522-1Kit 1Kit
EUR 750

Human vitronectin total antigen assay ELISA Kit
MBS135522-5x1Kit 5x1Kit
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Mouse vitronectin total antigen assay ELISA Kit
MBS135526-1Kit 1Kit
EUR 750

Mouse vitronectin total antigen assay ELISA Kit
MBS135526-5x1Kit 5x1Kit
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ELISA GENERAL ASSAY DILUENT
MBS238291-100mL 100mL
EUR 210

ELISA GENERAL ASSAY DILUENT
MBS238291-5x100mL 5x100mL
EUR 770

ELISA NEPTUNE ASSAY DILUENT
MBS238293-100mL 100mL
EUR 235

ELISA NEPTUNE ASSAY DILUENT
MBS238293-5x100mL 5x100mL
EUR 885

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