Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics
Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis.
Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels.
Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.
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 96 WELL PCR SEGMENTED PLATE |
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| PCR-96-SG-C | CORNING | 10/pk | EUR 145 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 Adapter for 96 well plate |
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| 3510147 | Atto | 1unit | EUR 5581 |
 Streptavidin coated 96-well Plate |
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| 6523-5 | Biovision | EUR 327 | |
 Black 96 Well Plate, 1EA |
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| X025-1EA | Arbor Assays | 1EA | EUR 73 |
) Polystyrene-bottomed 96-well plate (ELISA) |
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| CT361 | U-CyTech | 6 | EUR 73 |
) 96 well filter plate (960ul each well) |
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| SD5006 | Bio Basic | 12UNIT | EUR 96.54 |
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 96 WELL CLEAR PCR PLATE FOR ABI |
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| PCR-96-AB-C | CORNING | 10/pk | EUR 167 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
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| P-DW-12-HC | CORNING | 5/pk | EUR 152 |
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Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
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 Clear Coated 96 Well Plate, 1EA |
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| C107-1EA | Arbor Assays | 1EA | EUR 189 |
) 96 well filter plate (no filter) |
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| SD5006-EMPTY | Bio Basic | 12UNIT | EUR 87.41 |
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 ELISA Kit) Equine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Eq-48T | DL Develop | 48T | EUR 360 |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Equine Immunoglobulin G (IgG) ELISA Kit |
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| DLR-IgG-Eq-96T | DL Develop | 96T | EUR 457 |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Equine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Eq-48Tests | Reddot Biotech | 48 Tests | EUR 358 |
Equine Immunoglobulin G (IgG) ELISA Kit |
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| RDR-IgG-Eq-96Tests | Reddot Biotech | 96 Tests | EUR 490 |
 96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE. |
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| CP-AXYGEM-96-50 | CORNING | 10/pk | EUR 457 |
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Description: Crystallography Plates; Crystallography Plates - Axygen |
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 96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE |
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| PCR-96-HS-C | CORNING | 10/pk | EUR 159 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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) 96 well DNA plate with membrane (960ul each well) |
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| SD5007 | Bio Basic | 12UNIT | EUR 242.7 |
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) 96 well RNA plate with membrane (960ul each well) |
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| SD5009 | Bio Basic | 12UNIT | EUR 260.97 |
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 96 Well Breast Tumor Genomic DNA Plate |
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| D8235086-1 | Biochain | 1 plate | EUR 316 |
 96 Well Colon Tumor Genomic DNA Plate |
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| D8235090-1 | Biochain | 1 plate | EUR 316 |
 96 Well Lung Tumor Genomic DNA Plate |
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| D8235152-1 | Biochain | 1 plate | EUR 316 |
 96 Well Stomach Tumor Genomic DNA Plate |
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| D8235248-1 | Biochain | 1 plate | EUR 316 |
) Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
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| CT365 | U-CyTech | 5 | EUR 78 |
 96-Well Plate PCR Products Purification Kit |
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| BS365 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
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96-Well Plate PCR Products Purification Kit |
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| BS3652 | Bio Basic | 2xPlates, 192prep | EUR 155.16 |
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 96-Well Plate DNA Cleanup Miniprep Kit |
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| BS369 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
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96-Well Plate DNA Cleanup Miniprep Kit |
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| BS3692 | Bio Basic | 2xPlates, 192prep | EUR 155.16 |
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 96-Well Plate Plasmid DNA Miniprep Kit |
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| BS415 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
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96-Well Plate Plasmid DNA Miniprep Kit |
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| BS4152 | Bio Basic | 2XPlates, 192prep | EUR 155.16 |
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 96-Well Plate Viral DNA Miniprep Kit |
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| VT92032 | Bio Basic | 2XPlates, 192prep | EUR 413.22 |
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 2 x Clear 96 well Plate, 2EA |
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| X003-2EA | Arbor Assays | 2EA | EUR 65 |
 5 x Clear 96 well Plate, 5EA |
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| X003-5EA | Arbor Assays | 5EA | EUR 80 |
2 x Clear 96 well Plate, 2EA |
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| X018-2EA | Arbor Assays | 2EA | EUR 65 |
 4 x Clear 96 Well Plate, 4EA |
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| X018-4EA | Arbor Assays | 4EA | EUR 87 |
 Black Half Area 96 Well Plate, 1EA |
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| X023-1EA | Arbor Assays | 1EA | EUR 80 |
 Radius 96-Well Cell Migration Assay |
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| CBA-126 | Cell Biolabs | 96 assays | EUR 572 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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Radius 96-Well Cell Migration Assay |
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| CBA-126-5 | Cell Biolabs | 5 x 96 assays | EUR 2248 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
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 CytoSelect 96-well Cell Transformation Assay |
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| CBA-130 | Cell Biolabs | 96 assays | EUR 722 |
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Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
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CytoSelect 96-well Cell Transformation Assay |
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| CBA-130-5 | Cell Biolabs | 5 x 96 assays | EUR 2886 |
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Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
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) CytoSelect 96-well Phagocytosis Assay (Zymosan) |
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| CBA-224 | Cell Biolabs | 96 assays | EUR 722 |
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Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
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CytoSelect 96-well Phagocytosis Assay (Zymosan) |
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| CBA-224-5 | Cell Biolabs | 5 x 96 assays | EUR 2938 |
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Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
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 OxiSelect 96-Well Comet Assay Kit |
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| STA-355 | Cell Biolabs | 96 assays | EUR 427 |
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Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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OxiSelect 96-Well Comet Assay Kit |
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| STA-355-5 | Cell Biolabs | 5 x 96 assays | EUR 1633 |
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Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
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 OxiSelect 96-Well Comet Assay Slide |
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| STA-356 | Cell Biolabs | 1 slide | EUR 183 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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OxiSelect 96-Well Comet Assay Slide |
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| STA-356-5 | Cell Biolabs | 5 slides | EUR 519 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
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 96-Well ROCK Activity Assay Kit |
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| STA-416 | Cell Biolabs | 96 assays | EUR 856 |
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Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
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96-Well ROCK Activity Assay Kit |
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| STA-416-5 | Cell Biolabs | 5 x 96 assays | EUR 3524 |
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Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
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 1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
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| P-DW-12-HC-S | CORNING | 5/pk | EUR 207 |
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Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
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) SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included) |
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| ACT-SL-96 | ACTGene | EUR 647 | |
 Block for 96 well plate, WSC-2620/30 |
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| 4002659 | Atto | 1unit | EUR 382 |
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Description: Bl ock for WSC-2620 / 2630 |
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) PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
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| CT366 | U-CyTech | 5 | EUR 224 |
) 96-well Plasmid DNA Extraction Kit (1 plate) |
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| FAPWE-96001 | Favorgen | 1 prep | EUR 146 |
 96 Well Plate RNA Cleanup and Concentration Kit |
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| BRC1251 | Bio Basic | 2xPlates, 192prep | EUR 250.31 |
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 96-Well Plate Animal Genomic DNA Miniprep Kit |
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| BS437 | Bio Basic | 5XPlates, 480prep | EUR 362.98 |
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96-Well Plate Animal Genomic DNA Miniprep Kit |
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| BS4372 | Bio Basic | 2XPlates, 192prep | EUR 195.5 |
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 96 Well Plate Yeast Genomic DNA Miniprep Kit |
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| BS8357 | Bio Basic | 2XPlates, 192prep | EUR 195.5 |
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 96-Well Plate Plant Genomic DNA Miniprep Kit |
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| BS8361 | Bio Basic | 2XPlates, 192prep | EUR 195.5 |
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 96 Well Plate Blood Genomic DNA Miniprep Kit |
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| BT92031 | Bio Basic | 2Plates, 192prep | EUR 175.71 |
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96 Well Plate Blood Genomic DNA Miniprep Kit |
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| BT92032 | Bio Basic | 5Plates, 480prep | EUR 324.92 |
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 96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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| SK1292 | Bio Basic | 2XPlates, 192prep | EUR 175.71 |
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96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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| SK1295 | Bio Basic | 5XPlates, 480prep | EUR 324.92 |
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) 96 Well ExoELISA plate (12x8 well strips, pack of 10 plates) |
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| EXOEL-96P-1 | SBI | 10 Plates | EUR 213 |
) CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric) |
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| CBA-081 | Cell Biolabs | 96 assays | EUR 606 |
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Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included. |
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 96-well Checkpoint Kinase Activity Assay Kit |
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| STA-414 | Cell Biolabs | 96 assays | EUR 757 |
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Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
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96-well Checkpoint Kinase Activity Assay Kit |
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| STA-414-5 | Cell Biolabs | 5 x 96 assays | EUR 3060 |
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Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
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 96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit |
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| 25-224 | Genesee Scientific | 100 plates | EUR 299 |
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Description: Suitable for cell culture but only for suspension cells
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) CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays) |
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| CBA-320 | Cell Biolabs | 96 assays | EUR 537 |
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Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis. |
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 SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
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| 480130 | CORNING | 1/pk | EUR 223 |
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Description: Lab Equipment; Constant Temperature Equipment |
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 DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES |
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| 480131 | CORNING | 1/pk | EUR 218 |
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Description: Lab Equipment; Constant Temperature Equipment |
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 DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
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| 480132 | CORNING | 1/pk | EUR 218 |
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Description: Lab Equipment; Constant Temperature Equipment |
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 COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE |
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| 432071 | CORNING | 1/pk | EUR 345 |
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Description: BioCision; BioCision CoolBox |
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 Block for 96 deep-well plate, WSC-2620/30 |
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| 4002661 | Atto | 1unit | EUR 382 |
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Description: Bl ock for WSC-2620 / 2630 |
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 Adaptor for 96-well PCR plate, accessory for AgileSealer? |
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| APS-200-96PCR | ACTGene | EUR 351 | |
 Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA |
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| C075-1EA | Arbor Assays | 1EA | EUR 178 |
 Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA |
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| C223-1EA | Arbor Assays | 1EA | EUR 178 |
 Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA |
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| C140-1EA | Arbor Assays | 1EA | EUR 178 |
 96 Well PCR Plates, Fits ABI Fast Plate Machines |
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| GP615 | GenDepot | 25 plates | EUR 212 |
 96 Well Plate Bacterial Total RNA Miniprep Super Kit |
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| BS585 | Bio Basic | 5XPlates, 480prep | EUR 487.82 |
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96 Well Plate Bacterial Total RNA Miniprep Super Kit |
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| BS5852 | Bio Basic | 2XPlates, 192prep | EUR 250.31 |
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 Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
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| BS92028 | Bio Basic | 2XPlates, 192prep | EUR 212.25 |
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Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
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| BS92029 | Bio Basic | 5XPlates, 480prep | EUR 364.5 |
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 Goat anti-Mouse, Coated White 96 Well Plate, 1EA |
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| X010-1EA | Arbor Assays | 1EA | EUR 109 |
 Goat anti-Rabbit, Coated White 96 Well Plate, 1EA |
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| X014-1EA | Arbor Assays | 1EA | EUR 109 |
 Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA |
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| X016-1EA | Arbor Assays | 1EA | EUR 109 |
 2 x Black Half Area 96 Well Plate, 2EA |
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| X037-2EA | Arbor Assays | 2EA | EUR 94 |
 Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA |
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| X061-1EA | Arbor Assays | 1EA | EUR 109 |
 Donkey anti-Sheep, Coated White 96 Well Plate, 1EA |
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| X063-1EA | Arbor Assays | 1EA | EUR 109 |
 5 plates/pack) Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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| PRTA55-5P | Alpha Diagnostics | 1 PK | EUR 263 |
 5 plates/pack) Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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| PRTG65-5P | Alpha Diagnostics | 1 PK | EUR 263 |
 96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML |
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| PCR-96-AB-C-PBC | CORNING | 10/pk | EUR 195 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric |
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| CBA-112-COL | Cell Biolabs | 96 assays | EUR 757 |
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Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I. |
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 CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric |
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| CBA-112-LN | Cell Biolabs | 96 assays | EUR 757 |
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Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
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) CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) |
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| CBA-220 | Cell Biolabs | 96 assays | EUR 635 |
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Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
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) 96-well Cellular Senescence Assay (SA ?-Gal Activity) |
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| CBA-231 | Cell Biolabs | 120 assays | EUR 635 |
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Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
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96-well Cellular Senescence Assay (SA ?-Gal Activity) |
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| CBA-231-5 | Cell Biolabs | 5 x 120 assays | EUR 2648 |
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Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
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 Clear 384 Well Plate |
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| X105-1EA | Arbor Assays | 1EA | EUR 112 |
 Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA |
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| X011-1EA | Arbor Assays | 1EA | EUR 102 |
Repeatability of a Commercially Available ELISATest for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk
An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions.
The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later.
The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at -20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing.
The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively.
Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cut-off 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK™ has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.
Individual and herd-level milk ELISA test status for Johne’s disease in Ireland after correcting for non-disease-associated variables
Antibody-detecting tests for Mycobacterium avium ssp. paratuberculosis (MAP) have low sensitivity and imperfect specificity for detection of infection. Sensitivity increases as the disease progresses. Aside from infection status and stage of disease, several factors affect test performance.
These factors have not yet been studied in dairy cows producing lower volumes of milk with higher solids concentration, such as those managed in low-input, pasture-based production systems.
Furthermore, the effect of correcting for these associations on individual and herd test status is also unknown. The first objective of this study was to examine the relationship between MAP antibody response in milk and milk yield, somatic cell count (SCC), fat and protein contents, and stage of lactation in dairy cows enrolled in the national Johne’s Disease Control Programme (JDCP) in Ireland.
The second objective was to examine the effect of correcting the antibody response for these associations on the test status of individual cows and herds, given that individual tests are often used to define a herd’s status. Data were extracted for herds in the JDCP from January 2014 to December 2015 inclusive, consisting of 42,657 milk recordings from 18,569 cows across 187 dairy herds.
Two linear regression models were constructed to investigate the association between log-transformed MAP sample-to-positive ratio and milk recording data and in primi- and multiparous cows.
Days in milk was modeled as a B-spline in each model, and cow and herd were included as random effects. Across both models, natural log-transformed MAP antibody response was negatively associated with milk yield, positively associated with protein and fat production, and had a curvilinear association with log-transformed SCC.
The association between MAP antibody response and days in milk varied over the course of the lactation. However, when combined, these variables explained only 5.1% of the variation in the antibody response of the population. After correcting for these associations, 93 multiparous cows and 20 primiparous cows changed category (negative, suspect, or positive).
When considered at the herd-test level, out of a total of 531 herd tests, 1 herd changed from negative to positive, and 5 herds changed from positive to negative. This study provides useful information to aid in the interpretation of antibody results for herds testing animals for the presence of MAP infection. At an overall population level, correction of the serological response for non-disease-associated factors has the potential to change the status of only a small number of cows.
At the herd level, the proportion of herds changing status was minimal. However, depending on the implications of a herd-level serological diagnosis, consideration should be given to correcting for these non-disease-associated variables within the context of national JD control programs.
 Elisa Assay Kit) Adiponectin (rat) Elisa Assay Kit |
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| K4903-100 | Biovision | EUR 843 | |
 Elisa Assay Kit) Visfatin (human) Elisa Assay Kit |
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| K4907-100 | Biovision | EUR 985 | |
 Elisa Assay Kit) Adiponectin (human) Elisa Assay Kit |
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| K2192-96 | ApexBio | 96 assays | EUR 1017 |
 Elisa Assay Kit) Adiponectin (mouse) Elisa Assay Kit |
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| K2193-96 | ApexBio | 96 assays | EUR 1017 |
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
|
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 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV100PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV105PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV125PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
| CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
|
|||
 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
| CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
|
|||
 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
| CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
|
|||
) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|||
| CAS510A-KIT | SBI | 1 Kit | EUR 805 |
|
|||
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV200PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
|
|||
 Glycosaminoglycans Assay Kit |
|||
| 6022 | Chondrex | 1 kit | EUR 419.75 |
|
Description: Glycosaminoglycans Assay Kit |
|||
 DNA Assay Kit |
|||
| 6023 | Chondrex | 1 kit | EUR 180.5 |
|
Description: DNA Assay Kit |
|||
 Hemoglobin Assay Kit |
|||
| 6024 | Chondrex | 1 kit | EUR 180.5 |
|
Description: Hemoglobin Assay Kit |
|||
 Proteasome Assay Kit |
|||
| 55R-1341 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Proteasome in the research laboratory |
|||
 Calpain Assay Kit |
|||
| 55R-1342 | Fitzgerald | 100 assays | EUR 779 |
|
Description: Assay Kit for detection of Calpain in the research laboratory |
|||
 Glutathione Assay Kit |
|||
| 55R-1343 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Glutathione in the research laboratory |
|||
Glutathione Assay Kit |
|||
| 55R-1354 | Fitzgerald | 100 assays | EUR 895 |
|
Description: Assay Kit for detection of Glutathione activity in the research laboratory |
|||
 HDAC Assay Kit |
|||
| 55R-1371 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of HDAC in the research laboratory |
|||
HDAC Assay Kit |
|||
| 55R-1372 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of HDAC in the research laboratory |
|||
 HAT Assay Kit |
|||
| 55R-1373 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of HAT in the research laboratory |
|||
 SOD Assay Kit |
|||
| 55R-1374 | Fitzgerald | 100 assays | EUR 602 |
|
Description: Assay Kit for detection of SOD in the research laboratory |
|||
 HDAC3 Assay Kit |
|||
| 55R-1377 | Fitzgerald | 100 assays | EUR 693 |
|
Description: Assay Kit for detection of HDAC3 in the research laboratory |
|||
 HDAC8 Assay Kit |
|||
| 55R-1379 | Fitzgerald | 100 assays | EUR 689 |
|
Description: Assay Kit for detection of HDAC8 in the research laboratory |
|||
 ATP Assay Kit |
|||
| 55R-1380 | Fitzgerald | 100 assays | EUR 809 |
|
Description: Assay Kit for detection of ATP in the research laboratory |
|||
 ADP Assay Kit |
|||
| 55R-1381 | Fitzgerald | 100 assays | EUR 809 |
|
Description: Assay Kit for detection of ADP in the research laboratory |
|||
 FAD Assay Kit |
|||
| 55R-1382 | Fitzgerald | 100 assays | EUR 732 |
|
Description: Assay Kit for detection of FAD in the research laboratory |
|||
 PEP Assay Kit |
|||
| 55R-1384 | Fitzgerald | 100 assays | EUR 876 |
|
Description: Assay Kit for detection of PEP in the research laboratory |
|||
 Ammonia Assay Kit |
|||
| 55R-1388 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Ammonia in the research laboratory |
|||
 cAMP Assay Kit |
|||
| 55R-1389 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of cAMP activity in the research laboratory |
|||
 cGMP Assay Kit |
|||
| 55R-1390 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of cGMP activity in the research laboratory |
|||
 Urea Assay Kit |
|||
| 55R-1391 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Urea in the research laboratory |
|||
 Calcium Assay Kit |
|||
| 55R-1392 | Fitzgerald | 250 assays | EUR 586 |
|
Description: Assay Kit for detection of Calcium activity in the research laboratory |
|||
 Magnesium Assay Kit |
|||
| 55R-1393 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Magnesium in the research laboratory |
|||
 Zinc Assay Kit |
|||
| 55R-1394 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Zinc in the research laboratory |
|||
 Iron Assay Kit |
|||
| 55R-1395 | Fitzgerald | 100 assays | EUR 758 |
|
Description: Assay Kit for detection of Iron in the research laboratory |
|||
 Phosphate Assay Kit |
|||
| 55R-1400 | Fitzgerald | 500 assays | EUR 415 |
|
Description: Assay Kit for detection of Phosphate in the research laboratory |
|||
 JNK Assay Kit |
|||
| 55R-1407 | Fitzgerald | 40 assays | EUR 868 |
|
Description: Assay Kit for detection of JNK in the research laboratory |
|||
JNK Assay Kit |
|||
| 55R-1408 | Fitzgerald | 40 assays | EUR 979 |
|
Description: Assay Kit for detection of JNK in the research laboratory |
|||
 Akt Assay Kit |
|||
| 55R-1409 | Fitzgerald | 40 assays | EUR 979 |
|
Description: Assay Kit for detection of Akt in the research laboratory |
|||
Ammonia Assay Kit |
|||
| 55R-1410 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Ammonia in the research laboratory |
|||
 Cobalt Assay Kit |
|||
| 55R-1435 | Fitzgerald | 100 assays | EUR 621 |
|
Description: Assay Kit for detection of Cobalt in the research laboratory |
|||
 Nickel Assay Kit |
|||
| 55R-1436 | Fitzgerald | 100 assays | EUR 621 |
|
Description: Assay Kit for detection of Nickel in the research laboratory |
|||
 Chloride Assay Kit |
|||
| 55R-1437 | Fitzgerald | 100 assays | EUR 484 |
|
Description: Assay Kit for detection of Chloride in the research laboratory |
|||
 Aspartate Assay Kit |
|||
| 55R-1438 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Aspartate in the research laboratory |
|||
 Hydroxyproline Assay Kit |
|||
| 55R-1439 | Fitzgerald | 100 assays | EUR 844 |
|
Description: Assay Kit for detection of Hydroxyproline in the research laboratory |
|||
 Phenylalanine Assay Kit |
|||
| 55R-1442 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Phenylalanine in the research laboratory |
|||
 Phosphatidylcholine Assay Kit |
|||
| 55R-1443 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory |
|||
 Glucose Assay Kit |
|||
| 55R-1449 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Glucose in the research laboratory |
|||
 Lactate Assay Kit |
|||
| 55R-1450 | Fitzgerald | 100 assays | EUR 844 |
|
Description: Assay Kit for detection of Lactate in the research laboratory |
|||
 Pyruvate Assay Kit |
|||
| 55R-1452 | Fitzgerald | 100 assays | EUR 844 |
|
Description: Assay Kit for detection of Pyruvate in the research laboratory |
|||
 Adipogenesis Assay Kit |
|||
| 55R-1454 | Fitzgerald | 100 assays | EUR 586 |
|
Description: Assay Kit for detection of Adipogenesis in the research laboratory |
|||
Glucose Assay Kit |
|||
| 55R-1458 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Glucose in the research laboratory |
|||
 Galactose Assay Kit |
|||
| 55R-1459 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Galactose in the research laboratory |
|||
 Maltose Assay Kit |
|||
| 55R-1460 | Fitzgerald | 100 assays | EUR 723 |
|
Description: Assay Kit for detection of Maltose in the research laboratory |
|||
 Fructose Assay Kit |
|||
| 55R-1461 | Fitzgerald | 100 assays | EUR 689 |
|
Description: Assay Kit for detection of Fructose in the research laboratory |
|||
 Ethanol Assay Kit |
|||
| 55R-1462 | Fitzgerald | 100 assays | EUR 774 |
|
Description: Assay Kit for detection of Ethanol in the research laboratory |
|||
Galactose Assay Kit |
|||
| 55R-1463 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Galactose in the research laboratory |
|||
 Lactose Assay Kit |
|||
| 55R-1466 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Lactose in the research laboratory |
|||
 Creatinine Assay Kit |
|||
| 55R-1467 | Fitzgerald | 100 assays | EUR 638 |
|
Description: Assay Kit for detection of Creatinine in the research laboratory |
|||
 Sucrose Assay Kit |
|||
| 55R-1468 | Fitzgerald | 100 assays | EUR 689 |
|
Description: Assay Kit for detection of Sucrose in the research laboratory |
|||
Lactate Assay Kit |
|||
| 55R-1469 | Fitzgerald | 100 assays | EUR 844 |
|
Description: Assay Kit for detection of Lactate in the research laboratory |
|||
Maltose Assay Kit |
|||
| 55R-1470 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Maltose in the research laboratory |
|||
 Glutamate Assay Kit |
|||
| 55R-1471 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Glutamate in the research laboratory |
|||
 Fumarate Assay Kit |
|||
| 55R-1475 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Fumarate in the research laboratory |
|||
 Creatine Assay Kit |
|||
| 55R-1476 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Creatine in the research laboratory |
|||
 Sarcosine Assay Kit |
|||
| 55R-1477 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Sarcosine in the research laboratory |
|||
 Malate Assay Kit |
|||
| 55R-1478 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Malate in the research laboratory |
|||
 Glycogen Assay Kit |
|||
| 55R-1481 | Fitzgerald | 100 assays | EUR 844 |
|
Description: Assay Kit for detection of Glycogen in the research laboratory |
|||
 Starch Assay Kit |
|||
| 55R-1482 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Starch in the research laboratory |
|||
 Alanine Assay Kit |
|||
| 55R-1483 | Fitzgerald | 100 assays | EUR 654 |
|
Description: Assay Kit for detection of Alanine in the research laboratory |
|||
 Formate Assay Kit |
|||
| 55R-1484 | Fitzgerald | 100 assays | EUR 809 |
|
Description: Assay Kit for detection of Formate in the research laboratory |
|||
 Citrate Assay Kit |
|||
| 55R-1485 | Fitzgerald | 100 assays | EUR 740 |
|
Description: Assay Kit for detection of Citrate in the research laboratory |
|||
 Isocitrate Assay Kit |
|||
| 55R-1486 | Fitzgerald | 100 assays | EUR 663 |
|
Description: Assay Kit for detection of Isocitrate in the research laboratory |
|||
 Oxaloacetate Assay Kit |
|||
| 55R-1488 | Fitzgerald | 100 assays | EUR 663 |
|
Description: Assay Kit for detection of Oxaloacetate in the research laboratory |
|||
 Heme Assay Kit |
|||
| 55R-1492 | Fitzgerald | 100 assays | EUR 673 |
|
Description: Assay Kit for detection of Heme in the research laboratory |
|||
Glucose Assay Kit |
|||
| 55R-1494 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Glucose in the research laboratory |
|||
 Amylase Assay Kit |
|||
| 55R-1503 | Fitzgerald | 100 assays | EUR 551 |
|
Description: Assay Kit for detection of Amylase in the research laboratory |
|||
 Inosine Assay Kit |
|||
| 55R-1504 | Fitzgerald | 100 assays | EUR 732 |
|
Description: Assay Kit for detection of Inosine in the research laboratory |
|||
 Aconitase Assay Kit |
|||
| 55R-1505 | Fitzgerald | 100 assays | EUR 689 |
|
Description: Assay Kit for detection of Aconitase in the research laboratory |
|||
 Lipase Assay Kit |
|||
| 55R-1506 | Fitzgerald | 100 assays | EUR 706 |
|
Description: Assay Kit for detection of Lipase in the research laboratory |
|||