Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix

September 8, 2020 Leave a comment

Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics

Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis.
Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels.
Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.
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Black 96 Well Plate, 1EA
X025-1EA 1EA
EUR 73

Streptavidin coated 96-well Plate
6523-5
EUR 327

96 well filter plate (960ul each well)
SD5006 12UNIT
EUR 96.54

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96 well filter plate (no filter)
SD5006-EMPTY 12UNIT
EUR 87.41

Clear Coated 96 Well Plate, 1EA
C107-1EA 1EA
EUR 189

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE
PCR-96-HS-C 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.
CP-AXYGEM-96-50 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

Equine Immunoglobulin G (IgG) ELISA Kit
DLR-IgG-Eq-48T 48T
EUR 360
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit
DLR-IgG-Eq-96T 96T
EUR 457
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Immunoglobulin G (IgG) ELISA Kit
RDR-IgG-Eq-48Tests 48 Tests
EUR 358

Equine Immunoglobulin G (IgG) ELISA Kit
RDR-IgG-Eq-96Tests 96 Tests
EUR 490

96 well DNA plate with membrane (960ul each well)
SD5007 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)
SD5009 12UNIT
EUR 260.97

96-Well Plate PCR Products Purification Kit
BS365 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit
BS3652 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit
BS369 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit
BS3692 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit
BS415 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit
BS4152 2XPlates, 192prep
EUR 155.16

96-Well Plate Viral DNA Miniprep Kit
VT92032 2XPlates, 192prep
EUR 413.22

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365 5
EUR 78

2 x Clear 96 well Plate, 2EA
X003-2EA 2EA
EUR 65

5 x Clear 96 well Plate, 5EA
X003-5EA 5EA
EUR 80

2 x Clear 96 well Plate, 2EA
X018-2EA 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA
X018-4EA 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA
X023-1EA 1EA
EUR 80

96 Well Breast Tumor Genomic DNA Plate
D8235086-1 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate
D8235090-1 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate
D8235152-1 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1 1 plate
EUR 316

Radius 96-Well Cell Migration Assay
CBA-126 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay
CBA-126-5 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay
CBA-130 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay
CBA-130-5 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224-5 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Kit
STA-355 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit
STA-355-5 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide
STA-356 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit
STA-416 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit
STA-416-5 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)
ACT-SL-96
EUR 647

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032 5Plates, 480prep
EUR 324.92

Block for 96 well plate, WSC-2620/30
4002659 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295 5XPlates, 480prep
EUR 324.92

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366 5
EUR 224

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1 10 Plates
EUR 213

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)
CBA-081 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-well Checkpoint Kinase Activity Assay Kit
STA-414 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit
STA-414-5 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit
25-224 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P 1 PK
EUR 263

Adaptor for 96-well PCR plate, accessory for AgileSealer?
APS-200-96PCR
EUR 351

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029 5XPlates, 480prep
EUR 364.5

Block for 96 deep-well plate, WSC-2620/30
4002661 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE
432071 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480130 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES
480131 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480132 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well PCR Plates, Fits ABI Fast Plate Machines
GP615 25 plates
EUR 212

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA
C075-1EA 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA
C140-1EA 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA
C223-1EA 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA
X010-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA
X014-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA
X016-1EA 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA
X037-2EA 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA
X061-1EA 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA
X063-1EA 1EA
EUR 109

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)
CBA-320 96 assays
EUR 537
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML
PCR-96-AB-C-PBC 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well
1018-96 1/EA
EUR 518

Clear 384 Well Plate
X105-1EA 1EA
EUR 112

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric
CBA-112-COL 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric
CBA-112-LN 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)
CBA-220 96 assays
EUR 635
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231 120 assays
EUR 635
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231-5 5 x 120 assays
EUR 2648
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Repeatability of a Commercially Available ELISATest for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions.
The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later.
The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at -20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing.
The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively.
Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cut-off 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK™ has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.

Individual and herd-level milk ELISA test status for Johne’s disease in Ireland after correcting for non-disease-associated variables

Antibody-detecting tests for Mycobacterium avium ssp. paratuberculosis (MAP) have low sensitivity and imperfect specificity for detection of infection. Sensitivity increases as the disease progresses. Aside from infection status and stage of disease, several factors affect test performance.
These factors have not yet been studied in dairy cows producing lower volumes of milk with higher solids concentration, such as those managed in low-input, pasture-based production systems.
Furthermore, the effect of correcting for these associations on individual and herd test status is also unknown. The first objective of this study was to examine the relationship between MAP antibody response in milk and milk yield, somatic cell count (SCC), fat and protein contents, and stage of lactation in dairy cows enrolled in the national Johne’s Disease Control Programme (JDCP) in Ireland.
The second objective was to examine the effect of correcting the antibody response for these associations on the test status of individual cows and herds, given that individual tests are often used to define a herd’s status. Data were extracted for herds in the JDCP from January 2014 to December 2015 inclusive, consisting of 42,657 milk recordings from 18,569 cows across 187 dairy herds.
Two linear regression models were constructed to investigate the association between log-transformed MAP sample-to-positive ratio and milk recording data and in primi- and multiparous cows.
Days in milk was modeled as a B-spline in each model, and cow and herd were included as random effects. Across both models, natural log-transformed MAP antibody response was negatively associated with milk yield, positively associated with protein and fat production, and had a curvilinear association with log-transformed SCC.
The association between MAP antibody response and days in milk varied over the course of the lactation. However, when combined, these variables explained only 5.1% of the variation in the antibody response of the population. After correcting for these associations, 93 multiparous cows and 20 primiparous cows changed category (negative, suspect, or positive).
When considered at the herd-test level, out of a total of 531 herd tests, 1 herd changed from negative to positive, and 5 herds changed from positive to negative. This study provides useful information to aid in the interpretation of antibody results for herds testing animals for the presence of MAP infection. At an overall population level, correction of the serological response for non-disease-associated factors has the potential to change the status of only a small number of cows.
At the herd level, the proportion of herds changing status was minimal. However, depending on the implications of a herd-level serological diagnosis, consideration should be given to correcting for these non-disease-associated variables within the context of national JD control programs.

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT 1 Kit
EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT 1 Kit
EUR 1132

Lactose Assay Kit
MET-5001 100 assays
EUR 432
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe.

Bilirubin Assay Kit
MET-5010 200 assays
EUR 479
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus.

Pyruvate Assay Kit
MET-5029 100 assays
EUR 479
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.

Glycine Assay Kit
MET-5070 100 assays
EUR 450

Taurine Assay Kit
MET-5071 200 assays
EUR 508

Sarcosine Assay Kit
MET-5072 100 assays
EUR 450

Tyrosine Assay Kit
MET-5073 100 assays
EUR 450

Phospholipid Assay Kit
MET-5085 96 assays
EUR 456

Ammonia Assay Kit
MET-5086 100 assays
EUR 456

Adenosine Assay Kit
MET-5090 100 assays
EUR 508

Inosine Assay Kit
MET-5092 100 assays
EUR 508

Alanine Assay Kit
MET-5093 200 assays
EUR 508

Histamine Assay Kit
AKR-360 96 assays
EUR 519
Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve.

Glucose Assay Kit
abx090673-1Kit 1 Kit
EUR 237

ADA Assay Kit
abx090675-100tests 100 tests
EUR 237

Glutamate Assay Kit
abx096004-100Assays 100 Assays
EUR 441

Glutathione Assay Kit
abx096005-100Assays 100 Assays
EUR 378

Trehalase Assay Kit
abx096014-100Assays 100 Assays
EUR 551

Pyruvate Assay Kit
abx097982-100Assays 100 Assays
EUR 472

NADPase Assay Kit
abx097983-100Assays 100 Assays
EUR 504

Starch Assay Kit
abx097988-100Assays 100 Assays
EUR 441

Trehalose Assay Kit
abx097995-100Assays 100 Assays
EUR 472

ADA Assay Kit
abx098403-Hitachi7060R190ml2R290ml1 Hitachi 7060; R1: 90ml×2 R2: 90ml×1
EUR 739

ADA Assay Kit
abx098403-Hitachi7170R140ml4R220ml4 Hitachi 7170; R1: 40ml×4 R2: 20ml×4
EUR 801

ADA Assay Kit
abx098403-Hitachi7170R160ml4R260ml2 Hitachi 7170; R1: 60ml×4 R2: 60ml×2
EUR 911

ADA Assay Kit
abx098403-Toshiba40R150ml4R250ml2 Toshiba 40; R1: 50ml×4 R2: 50ml×2
EUR 786

Calcium Assay Kit
abx098414-Hitachi7020R140ml2R240ml2 Hitachi 7020; R1: 40ml×2 R2: 40ml×2
EUR 206

Calcium Assay Kit
abx098414-Hitachi7060R190ml1R290ml1 Hitachi 7060; R1: 90ml×1 R2: 90ml×1
EUR 206

Calcium Assay Kit
abx098414-Toshiba40R140ml2R240ml2 Toshiba 40; R1: 40ml×2 R2: 40ml×2
EUR 206

Calcium Assay Kit
abx098414-UniversalR140ml2R240ml2 Universal; R1: 40ml×2 R2: 40ml×2
EUR 206

Cholinesterase Assay Kit
abx098416-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 300

Cholinesterase Assay Kit
abx098416-Hitachi7170R140ml3R230ml1 Hitachi 7170; R1: 40ml×3 R2: 30ml×1
EUR 316

Cholinesterase Assay Kit
abx098416-Hitachi7170R160ml2R230ml1 Hitachi 7170; R1: 60ml×2 R2: 30ml×1
EUR 316

Cholinesterase Assay Kit
abx098416-Toshiba40R140ml4R240ml1 Toshiba 40; R1: 40ml×4 R2: 40ml×1
EUR 331

Cholinesterase Assay Kit
abx098416-UniversalR160ml2R215ml2 Universal; R1: 60ml×2 R2: 15ml×2
EUR 316

Creatinine Assay Kit
abx098422-Hitachi7020R150ml3R250ml1 Hitachi 7020; R1: 50ml×3 R2: 50ml×1
EUR 519

Creatinine Assay Kit
abx098422-Hitachi7060R190ml2R260ml1 Hitachi 7060; R1: 90ml×2 R2: 60ml×1
EUR 472

Creatinine Assay Kit
abx098422-Toshiba120R140ml3R240ml1 Toshiba 120; R1: 40ml×3 R2: 40ml×1
EUR 566

Creatinine Assay Kit
abx098422-Toshiba120R150ml3R250ml1 Toshiba 120; R1: 50ml×3 R2: 50ml×1
EUR 519

Creatinine Assay Kit
abx098422-Toshiba40R150ml3R250ml1 Toshiba 40; R1: 50ml×3 R2: 50ml×1
EUR 519

Fructosamine Assay Kit
abx098427-Hitachi7020R140ml2R220ml1 Hitachi 7020; R1: 40ml×2 R2: 20ml×1
EUR 253

Fructosamine Assay Kit
abx098427-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 253

Fructosamine Assay Kit
abx098427-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 206

Fructosamine Assay Kit
abx098427-Hitachi7170R140ml12R240ml3 Hitachi 7170; R1: 40ml×12 R2: 40ml×3
EUR 222

Fructosamine Assay Kit
abx098427-Hitachi7170R140ml2R220ml1 Hitachi 7170; R1: 40ml×2 R2: 20ml×1
EUR 253

Glucose Assay Kit
abx098429-Hitachi7020R140ml4R240ml1 Hitachi 7020; R1: 40ml×4 R2: 40ml×1
EUR 222

Glucose Assay Kit
abx098429-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 222

Glucose Assay Kit
abx098429-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 222

Haptoglobin Assay Kit
abx098432-BeckmanR140ml1R210ml1 Beckman; R1: 40ml×1 R2: 10ml×1
EUR 347

Haptoglobin Assay Kit
abx098432-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1: 40ml×1 R2: 10ml×1
EUR 347

Haptoglobin Assay Kit
abx098432-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 269

Haptoglobin Assay Kit
abx098432-Toshiba40R140ml1R210ml1 Toshiba 40; R1: 40ml×1 R2: 10ml×1
EUR 347

Homocysteine Assay Kit
abx098434-Hitachi7060R160ml1R210ml1 Hitachi 7060; R1: 60ml×1 R2: 10ml×1
EUR 1036

Homocysteine Assay Kit
abx098434-Hitachi7170R130ml2R210ml1 Hitachi 7170; R1: 30ml×2 R2: 10ml×1
EUR 1036

Homocysteine Assay Kit
abx098434-Toshiba40R130ml2R210ml1 Toshiba 40; R1: 30ml×2 R2: 10ml×1
EUR 1036

Homocysteine Assay Kit
abx098434-UniversalR130ml2R210ml1 Universal; R1: 30ml×2 R2: 10ml×1
EUR 1036

Iron Assay Kit
abx098439-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1:40ml×1 R2:10ml×1
EUR 206

Iron Assay Kit
abx098439-Hitachi7170R140ml1R210ml1 Hitachi 7170; R1:40ml×1 R2:10ml×1
EUR 206

Iron Assay Kit
abx098439-Hitachi7170R160ml1R215ml1 Hitachi 7170; R1:60ml×1 R2:15ml×1
EUR 222

Iron Assay Kit
abx098439-Toshiba40R140ml1R210ml1 Toshiba 40; R1:40ml×1 R2:10ml×1
EUR 206

Lipase Assay Kit
abx098442-BeckmanR180ml1R220ml1 Beckman; R1:80ml×1 R2:20ml×1
EUR 692

Lipase Assay Kit
abx098442-Hitachi7170R140ml2R220ml1 Hitachi 7170; R1:40ml×2 R2:20ml×1
EUR 692

Lipase Assay Kit
abx098442-Hitachi7170R160ml1R215ml1 Hitachi 7170; R1:60ml×1 R2:15ml×1
EUR 566

Lipase Assay Kit
abx098442-Toshiba40R140ml1R210ml1 Toshiba 40; R1:40ml×1 R2:10ml×1
EUR 441

Lipase Assay Kit
abx098442-UniversalR120ml1R25ml1 Universal; R1:20ml×1 R2:5ml×1
EUR 316

Magnesium Assay Kit
abx098445-Hitachi7020R140ml2R240ml2 Hitachi 7020; R1: 40ml×2 R2: 40ml×2
EUR 206

Magnesium Assay Kit
abx098445-Hitachi7060R190ml1R290ml1 Hitachi 7060; R1: 90ml×1 R2: 90ml×1
EUR 206

Magnesium Assay Kit
abx098445-Toshiba40R140ml2R240ml2 Toshiba 40; R1: 40ml×2 R2: 40ml×2
EUR 206

Magnesium Assay Kit
abx098445-Toshiba40R150ml2R250ml2 Toshiba 40; R1: 50ml×2 R2: 50ml×2
EUR 206

Microalbumin Assay KIt
abx098446-Hitachi7060R150ml1R210ml1 Hitachi 7060; R1: 50ml×1 R2: 10ml×1
EUR 363

Microalbumin Assay KIt
abx098446-Hitachi7060R150ml3R230ml1 Hitachi 7060; R1: 50ml×3 R2: 30ml×1
EUR 363

Microalbumin Assay KIt
abx098446-Hitachi7170R125ml1R25ml1 Hitachi 7170; R1: 25ml×1 R2: 5ml×1
EUR 269

Microalbumin Assay KIt
abx098446-Hitachi7170R150ml1R210ml1 Hitachi 7170; R1: 50ml×1 R2: 10ml×1
EUR 363

Myoglobin Assay Kit
abx098448-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1: 40ml×1 R2: 10ml×1
EUR 1193

Myoglobin Assay Kit
abx098448-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 692

Myoglobin Assay Kit
abx098448-Hitachi7170R140ml1R210ml1 Hitachi 7170; R1: 40ml×1 R2: 10ml×1
EUR 1193

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