Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics
Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis.
Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels.
Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.

linkownia
Black 96 Well Plate, 1EA |
|||
X025-1EA | Arbor Assays | 1EA | EUR 73 |
Streptavidin coated 96-well Plate |
|||
6523-5 | Biovision | EUR 327 |
96 well filter plate (960ul each well) |
|||
SD5006 | Bio Basic | 12UNIT | EUR 96.54 |
96 WELL CLEAR PCR PLATE FOR ABI |
|||
PCR-96-AB-C | CORNING | 10/pk | EUR 167 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
|||
P-DW-12-HC | CORNING | 5/pk | EUR 152 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
96 well filter plate (no filter) |
|||
SD5006-EMPTY | Bio Basic | 12UNIT | EUR 87.41 |
Clear Coated 96 Well Plate, 1EA |
|||
C107-1EA | Arbor Assays | 1EA | EUR 189 |
96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE |
|||
PCR-96-HS-C | CORNING | 10/pk | EUR 159 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE. |
|||
CP-AXYGEM-96-50 | CORNING | 10/pk | EUR 457 |
Description: Crystallography Plates; Crystallography Plates - Axygen |
Equine Immunoglobulin G (IgG) ELISA Kit |
|||
DLR-IgG-Eq-48T | DL Develop | 48T | EUR 360 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Equine Immunoglobulin G (IgG) ELISA Kit |
|||
DLR-IgG-Eq-96T | DL Develop | 96T | EUR 457 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Equine Immunoglobulin G (IgG) ELISA Kit |
|||
RDR-IgG-Eq-48Tests | Reddot Biotech | 48 Tests | EUR 358 |
Equine Immunoglobulin G (IgG) ELISA Kit |
|||
RDR-IgG-Eq-96Tests | Reddot Biotech | 96 Tests | EUR 490 |
96 well DNA plate with membrane (960ul each well) |
|||
SD5007 | Bio Basic | 12UNIT | EUR 242.7 |
96 well RNA plate with membrane (960ul each well) |
|||
SD5009 | Bio Basic | 12UNIT | EUR 260.97 |
96-Well Plate PCR Products Purification Kit |
|||
BS365 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
96-Well Plate PCR Products Purification Kit |
|||
BS3652 | Bio Basic | 2xPlates, 192prep | EUR 155.16 |
96-Well Plate DNA Cleanup Miniprep Kit |
|||
BS369 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
96-Well Plate DNA Cleanup Miniprep Kit |
|||
BS3692 | Bio Basic | 2xPlates, 192prep | EUR 155.16 |
96-Well Plate Plasmid DNA Miniprep Kit |
|||
BS415 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
96-Well Plate Plasmid DNA Miniprep Kit |
|||
BS4152 | Bio Basic | 2XPlates, 192prep | EUR 155.16 |
96-Well Plate Viral DNA Miniprep Kit |
|||
VT92032 | Bio Basic | 2XPlates, 192prep | EUR 413.22 |
Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
|||
CT365 | U-CyTech | 5 | EUR 78 |
2 x Clear 96 well Plate, 2EA |
|||
X003-2EA | Arbor Assays | 2EA | EUR 65 |
5 x Clear 96 well Plate, 5EA |
|||
X003-5EA | Arbor Assays | 5EA | EUR 80 |
2 x Clear 96 well Plate, 2EA |
|||
X018-2EA | Arbor Assays | 2EA | EUR 65 |
4 x Clear 96 Well Plate, 4EA |
|||
X018-4EA | Arbor Assays | 4EA | EUR 87 |
Black Half Area 96 Well Plate, 1EA |
|||
X023-1EA | Arbor Assays | 1EA | EUR 80 |
96 Well Breast Tumor Genomic DNA Plate |
|||
D8235086-1 | Biochain | 1 plate | EUR 316 |
96 Well Colon Tumor Genomic DNA Plate |
|||
D8235090-1 | Biochain | 1 plate | EUR 316 |
96 Well Lung Tumor Genomic DNA Plate |
|||
D8235152-1 | Biochain | 1 plate | EUR 316 |
96 Well Stomach Tumor Genomic DNA Plate |
|||
D8235248-1 | Biochain | 1 plate | EUR 316 |
Radius 96-Well Cell Migration Assay |
|||
CBA-126 | Cell Biolabs | 96 assays | EUR 572 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
|||
CBA-126-5 | Cell Biolabs | 5 x 96 assays | EUR 2248 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
CytoSelect 96-well Cell Transformation Assay |
|||
CBA-130 | Cell Biolabs | 96 assays | EUR 722 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Cell Transformation Assay |
|||
CBA-130-5 | Cell Biolabs | 5 x 96 assays | EUR 2886 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
|||
CBA-224 | Cell Biolabs | 96 assays | EUR 722 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
|||
CBA-224-5 | Cell Biolabs | 5 x 96 assays | EUR 2938 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
OxiSelect 96-Well Comet Assay Kit |
|||
STA-355 | Cell Biolabs | 96 assays | EUR 427 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Kit |
|||
STA-355-5 | Cell Biolabs | 5 x 96 assays | EUR 1633 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Slide |
|||
STA-356 | Cell Biolabs | 1 slide | EUR 183 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
OxiSelect 96-Well Comet Assay Slide |
|||
STA-356-5 | Cell Biolabs | 5 slides | EUR 519 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
96-Well ROCK Activity Assay Kit |
|||
STA-416 | Cell Biolabs | 96 assays | EUR 856 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
96-Well ROCK Activity Assay Kit |
|||
STA-416-5 | Cell Biolabs | 5 x 96 assays | EUR 3524 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
|||
P-DW-12-HC-S | CORNING | 5/pk | EUR 207 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included) |
|||
ACT-SL-96 | ACTGene | EUR 647 |
96-well Plasmid DNA Extraction Kit (1 plate) |
|||
FAPWE-96001 | Favorgen | 1 prep | EUR 146 |
96 Well Plate RNA Cleanup and Concentration Kit |
|||
BRC1251 | Bio Basic | 2xPlates, 192prep | EUR 250.31 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
|||
BS437 | Bio Basic | 5XPlates, 480prep | EUR 362.98 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
|||
BS4372 | Bio Basic | 2XPlates, 192prep | EUR 195.5 |
96 Well Plate Yeast Genomic DNA Miniprep Kit |
|||
BS8357 | Bio Basic | 2XPlates, 192prep | EUR 195.5 |
96-Well Plate Plant Genomic DNA Miniprep Kit |
|||
BS8361 | Bio Basic | 2XPlates, 192prep | EUR 195.5 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
|||
BT92031 | Bio Basic | 2Plates, 192prep | EUR 175.71 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
|||
BT92032 | Bio Basic | 5Plates, 480prep | EUR 324.92 |
Block for 96 well plate, WSC-2620/30 |
|||
4002659 | Atto | 1unit | EUR 382 |
Description: Bl ock for WSC-2620 / 2630 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
|||
SK1292 | Bio Basic | 2XPlates, 192prep | EUR 175.71 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
|||
SK1295 | Bio Basic | 5XPlates, 480prep | EUR 324.92 |
PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
|||
CT366 | U-CyTech | 5 | EUR 224 |
96 Well ExoELISA plate (12x8 well strips, pack of 10 plates) |
|||
EXOEL-96P-1 | SBI | 10 Plates | EUR 213 |
CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric) |
|||
CBA-081 | Cell Biolabs | 96 assays | EUR 606 |
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included. |
96-well Checkpoint Kinase Activity Assay Kit |
|||
STA-414 | Cell Biolabs | 96 assays | EUR 757 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-well Checkpoint Kinase Activity Assay Kit |
|||
STA-414-5 | Cell Biolabs | 5 x 96 assays | EUR 3060 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit |
|||
25-224 | Genesee Scientific | 100 plates | EUR 299 |
Description: Suitable for cell culture but only for suspension cells
|
Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
|||
PRTA55-5P | Alpha Diagnostics | 1 PK | EUR 263 |
Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
|||
PRTG65-5P | Alpha Diagnostics | 1 PK | EUR 263 |
Adaptor for 96-well PCR plate, accessory for AgileSealer? |
|||
APS-200-96PCR | ACTGene | EUR 351 |
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
|||
BS585 | Bio Basic | 5XPlates, 480prep | EUR 487.82 |
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
|||
BS5852 | Bio Basic | 2XPlates, 192prep | EUR 250.31 |
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
|||
BS92028 | Bio Basic | 2XPlates, 192prep | EUR 212.25 |
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
|||
BS92029 | Bio Basic | 5XPlates, 480prep | EUR 364.5 |
Block for 96 deep-well plate, WSC-2620/30 |
|||
4002661 | Atto | 1unit | EUR 382 |
Description: Bl ock for WSC-2620 / 2630 |
COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE |
|||
432071 | CORNING | 1/pk | EUR 345 |
Description: BioCision; BioCision CoolBox |
SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
|||
480130 | CORNING | 1/pk | EUR 223 |
Description: Lab Equipment; Constant Temperature Equipment |
DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES |
|||
480131 | CORNING | 1/pk | EUR 218 |
Description: Lab Equipment; Constant Temperature Equipment |
DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
|||
480132 | CORNING | 1/pk | EUR 218 |
Description: Lab Equipment; Constant Temperature Equipment |
96 Well PCR Plates, Fits ABI Fast Plate Machines |
|||
GP615 | GenDepot | 25 plates | EUR 212 |
Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA |
|||
C075-1EA | Arbor Assays | 1EA | EUR 178 |
Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA |
|||
C140-1EA | Arbor Assays | 1EA | EUR 178 |
Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA |
|||
C223-1EA | Arbor Assays | 1EA | EUR 178 |
Goat anti-Mouse, Coated White 96 Well Plate, 1EA |
|||
X010-1EA | Arbor Assays | 1EA | EUR 109 |
Goat anti-Rabbit, Coated White 96 Well Plate, 1EA |
|||
X014-1EA | Arbor Assays | 1EA | EUR 109 |
Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA |
|||
X016-1EA | Arbor Assays | 1EA | EUR 109 |
2 x Black Half Area 96 Well Plate, 2EA |
|||
X037-2EA | Arbor Assays | 2EA | EUR 94 |
Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA |
|||
X061-1EA | Arbor Assays | 1EA | EUR 109 |
Donkey anti-Sheep, Coated White 96 Well Plate, 1EA |
|||
X063-1EA | Arbor Assays | 1EA | EUR 109 |
CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays) |
|||
CBA-320 | Cell Biolabs | 96 assays | EUR 537 |
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis. |
96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML |
|||
PCR-96-AB-C-PBC | CORNING | 10/pk | EUR 195 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well |
|||
1018-96 | Intact Genomics | 1/EA | EUR 518 |
Clear 384 Well Plate |
|||
X105-1EA | Arbor Assays | 1EA | EUR 112 |
CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric |
|||
CBA-112-COL | Cell Biolabs | 96 assays | EUR 757 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I. |
CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric |
|||
CBA-112-LN | Cell Biolabs | 96 assays | EUR 757 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) |
|||
CBA-220 | Cell Biolabs | 96 assays | EUR 635 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
96-well Cellular Senescence Assay (SA ?-Gal Activity) |
|||
CBA-231 | Cell Biolabs | 120 assays | EUR 635 |
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
96-well Cellular Senescence Assay (SA ?-Gal Activity) |
|||
CBA-231-5 | Cell Biolabs | 5 x 120 assays | EUR 2648 |
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader. |
Repeatability of a Commercially Available ELISATest for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk
An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions.
The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later.
The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at -20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing.
The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively.
Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cut-off 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK™ has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.
Individual and herd-level milk ELISA test status for Johne’s disease in Ireland after correcting for non-disease-associated variables
Antibody-detecting tests for Mycobacterium avium ssp. paratuberculosis (MAP) have low sensitivity and imperfect specificity for detection of infection. Sensitivity increases as the disease progresses. Aside from infection status and stage of disease, several factors affect test performance.
These factors have not yet been studied in dairy cows producing lower volumes of milk with higher solids concentration, such as those managed in low-input, pasture-based production systems.
Furthermore, the effect of correcting for these associations on individual and herd test status is also unknown. The first objective of this study was to examine the relationship between MAP antibody response in milk and milk yield, somatic cell count (SCC), fat and protein contents, and stage of lactation in dairy cows enrolled in the national Johne’s Disease Control Programme (JDCP) in Ireland.
The second objective was to examine the effect of correcting the antibody response for these associations on the test status of individual cows and herds, given that individual tests are often used to define a herd’s status. Data were extracted for herds in the JDCP from January 2014 to December 2015 inclusive, consisting of 42,657 milk recordings from 18,569 cows across 187 dairy herds.
Two linear regression models were constructed to investigate the association between log-transformed MAP sample-to-positive ratio and milk recording data and in primi- and multiparous cows.
Days in milk was modeled as a B-spline in each model, and cow and herd were included as random effects. Across both models, natural log-transformed MAP antibody response was negatively associated with milk yield, positively associated with protein and fat production, and had a curvilinear association with log-transformed SCC.
The association between MAP antibody response and days in milk varied over the course of the lactation. However, when combined, these variables explained only 5.1% of the variation in the antibody response of the population. After correcting for these associations, 93 multiparous cows and 20 primiparous cows changed category (negative, suspect, or positive).
When considered at the herd-test level, out of a total of 531 herd tests, 1 herd changed from negative to positive, and 5 herds changed from positive to negative. This study provides useful information to aid in the interpretation of antibody results for herds testing animals for the presence of MAP infection. At an overall population level, correction of the serological response for non-disease-associated factors has the potential to change the status of only a small number of cows.
At the herd level, the proportion of herds changing status was minimal. However, depending on the implications of a herd-level serological diagnosis, consideration should be given to correcting for these non-disease-associated variables within the context of national JD control programs.
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV105PA-KIT | SBI | 1 Kit | EUR 1132 |
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV125PA-KIT | SBI | 1 Kit | EUR 1132 |
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|||
CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|||
CAS510A-KIT | SBI | 1 Kit | EUR 805 |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV200PA-KIT | SBI | 1 Kit | EUR 1132 |
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
Lactose Assay Kit |
|||
MET-5001 | Cell Biolabs | 100 assays | EUR 432 |
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe. |
Bilirubin Assay Kit |
|||
MET-5010 | Cell Biolabs | 200 assays | EUR 479 |
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus. |
Pyruvate Assay Kit |
|||
MET-5029 | Cell Biolabs | 100 assays | EUR 479 |
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve. |
Glycine Assay Kit |
|||
MET-5070 | Cell Biolabs | 100 assays | EUR 450 |
Taurine Assay Kit |
|||
MET-5071 | Cell Biolabs | 200 assays | EUR 508 |
Sarcosine Assay Kit |
|||
MET-5072 | Cell Biolabs | 100 assays | EUR 450 |
Tyrosine Assay Kit |
|||
MET-5073 | Cell Biolabs | 100 assays | EUR 450 |
Phospholipid Assay Kit |
|||
MET-5085 | Cell Biolabs | 96 assays | EUR 456 |
Ammonia Assay Kit |
|||
MET-5086 | Cell Biolabs | 100 assays | EUR 456 |
Adenosine Assay Kit |
|||
MET-5090 | Cell Biolabs | 100 assays | EUR 508 |
Inosine Assay Kit |
|||
MET-5092 | Cell Biolabs | 100 assays | EUR 508 |
Alanine Assay Kit |
|||
MET-5093 | Cell Biolabs | 200 assays | EUR 508 |
Histamine Assay Kit |
|||
AKR-360 | Cell Biolabs | 96 assays | EUR 519 |
Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve. |
Glucose Assay Kit |
|||
abx090673-1Kit | Abbexa | 1 Kit | EUR 237 |
ADA Assay Kit |
|||
abx090675-100tests | Abbexa | 100 tests | EUR 237 |
Glutamate Assay Kit |
|||
abx096004-100Assays | Abbexa | 100 Assays | EUR 441 |
Glutathione Assay Kit |
|||
abx096005-100Assays | Abbexa | 100 Assays | EUR 378 |
Trehalase Assay Kit |
|||
abx096014-100Assays | Abbexa | 100 Assays | EUR 551 |
Pyruvate Assay Kit |
|||
abx097982-100Assays | Abbexa | 100 Assays | EUR 472 |
NADPase Assay Kit |
|||
abx097983-100Assays | Abbexa | 100 Assays | EUR 504 |
Starch Assay Kit |
|||
abx097988-100Assays | Abbexa | 100 Assays | EUR 441 |
Trehalose Assay Kit |
|||
abx097995-100Assays | Abbexa | 100 Assays | EUR 472 |
ADA Assay Kit |
|||
abx098403-Hitachi7060R190ml2R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 90ml×1 | EUR 739 |
ADA Assay Kit |
|||
abx098403-Hitachi7170R140ml4R220ml4 | Abbexa | Hitachi 7170; R1: 40ml×4 R2: 20ml×4 | EUR 801 |
ADA Assay Kit |
|||
abx098403-Hitachi7170R160ml4R260ml2 | Abbexa | Hitachi 7170; R1: 60ml×4 R2: 60ml×2 | EUR 911 |
ADA Assay Kit |
|||
abx098403-Toshiba40R150ml4R250ml2 | Abbexa | Toshiba 40; R1: 50ml×4 R2: 50ml×2 | EUR 786 |
Calcium Assay Kit |
|||
abx098414-Hitachi7020R140ml2R240ml2 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
Calcium Assay Kit |
|||
abx098414-Hitachi7060R190ml1R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×1 R2: 90ml×1 | EUR 206 |
Calcium Assay Kit |
|||
abx098414-Toshiba40R140ml2R240ml2 | Abbexa | Toshiba 40; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
Calcium Assay Kit |
|||
abx098414-UniversalR140ml2R240ml2 | Abbexa | Universal; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
Cholinesterase Assay Kit |
|||
abx098416-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 300 |
Cholinesterase Assay Kit |
|||
abx098416-Hitachi7170R140ml3R230ml1 | Abbexa | Hitachi 7170; R1: 40ml×3 R2: 30ml×1 | EUR 316 |
Cholinesterase Assay Kit |
|||
abx098416-Hitachi7170R160ml2R230ml1 | Abbexa | Hitachi 7170; R1: 60ml×2 R2: 30ml×1 | EUR 316 |
Cholinesterase Assay Kit |
|||
abx098416-Toshiba40R140ml4R240ml1 | Abbexa | Toshiba 40; R1: 40ml×4 R2: 40ml×1 | EUR 331 |
Cholinesterase Assay Kit |
|||
abx098416-UniversalR160ml2R215ml2 | Abbexa | Universal; R1: 60ml×2 R2: 15ml×2 | EUR 316 |
Creatinine Assay Kit |
|||
abx098422-Hitachi7020R150ml3R250ml1 | Abbexa | Hitachi 7020; R1: 50ml×3 R2: 50ml×1 | EUR 519 |
Creatinine Assay Kit |
|||
abx098422-Hitachi7060R190ml2R260ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 60ml×1 | EUR 472 |
Creatinine Assay Kit |
|||
abx098422-Toshiba120R140ml3R240ml1 | Abbexa | Toshiba 120; R1: 40ml×3 R2: 40ml×1 | EUR 566 |
Creatinine Assay Kit |
|||
abx098422-Toshiba120R150ml3R250ml1 | Abbexa | Toshiba 120; R1: 50ml×3 R2: 50ml×1 | EUR 519 |
Creatinine Assay Kit |
|||
abx098422-Toshiba40R150ml3R250ml1 | Abbexa | Toshiba 40; R1: 50ml×3 R2: 50ml×1 | EUR 519 |
Fructosamine Assay Kit |
|||
abx098427-Hitachi7020R140ml2R220ml1 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 20ml×1 | EUR 253 |
Fructosamine Assay Kit |
|||
abx098427-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 253 |
Fructosamine Assay Kit |
|||
abx098427-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 206 |
Fructosamine Assay Kit |
|||
abx098427-Hitachi7170R140ml12R240ml3 | Abbexa | Hitachi 7170; R1: 40ml×12 R2: 40ml×3 | EUR 222 |
Fructosamine Assay Kit |
|||
abx098427-Hitachi7170R140ml2R220ml1 | Abbexa | Hitachi 7170; R1: 40ml×2 R2: 20ml×1 | EUR 253 |
Glucose Assay Kit |
|||
abx098429-Hitachi7020R140ml4R240ml1 | Abbexa | Hitachi 7020; R1: 40ml×4 R2: 40ml×1 | EUR 222 |
Glucose Assay Kit |
|||
abx098429-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 222 |
Glucose Assay Kit |
|||
abx098429-Hitachi7170R132ml4R28ml4 | Abbexa | Hitachi 7170; R1: 32ml×4 R2: 8ml×4 | EUR 222 |
Haptoglobin Assay Kit |
|||
abx098432-BeckmanR140ml1R210ml1 | Abbexa | Beckman; R1: 40ml×1 R2: 10ml×1 | EUR 347 |
Haptoglobin Assay Kit |
|||
abx098432-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1: 40ml×1 R2: 10ml×1 | EUR 347 |
Haptoglobin Assay Kit |
|||
abx098432-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 269 |
Haptoglobin Assay Kit |
|||
abx098432-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1: 40ml×1 R2: 10ml×1 | EUR 347 |
Homocysteine Assay Kit |
|||
abx098434-Hitachi7060R160ml1R210ml1 | Abbexa | Hitachi 7060; R1: 60ml×1 R2: 10ml×1 | EUR 1036 |
Homocysteine Assay Kit |
|||
abx098434-Hitachi7170R130ml2R210ml1 | Abbexa | Hitachi 7170; R1: 30ml×2 R2: 10ml×1 | EUR 1036 |
Homocysteine Assay Kit |
|||
abx098434-Toshiba40R130ml2R210ml1 | Abbexa | Toshiba 40; R1: 30ml×2 R2: 10ml×1 | EUR 1036 |
Homocysteine Assay Kit |
|||
abx098434-UniversalR130ml2R210ml1 | Abbexa | Universal; R1: 30ml×2 R2: 10ml×1 | EUR 1036 |
Iron Assay Kit |
|||
abx098439-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1:40ml×1 R2:10ml×1 | EUR 206 |
Iron Assay Kit |
|||
abx098439-Hitachi7170R140ml1R210ml1 | Abbexa | Hitachi 7170; R1:40ml×1 R2:10ml×1 | EUR 206 |
Iron Assay Kit |
|||
abx098439-Hitachi7170R160ml1R215ml1 | Abbexa | Hitachi 7170; R1:60ml×1 R2:15ml×1 | EUR 222 |
Iron Assay Kit |
|||
abx098439-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1:40ml×1 R2:10ml×1 | EUR 206 |
Lipase Assay Kit |
|||
abx098442-BeckmanR180ml1R220ml1 | Abbexa | Beckman; R1:80ml×1 R2:20ml×1 | EUR 692 |
Lipase Assay Kit |
|||
abx098442-Hitachi7170R140ml2R220ml1 | Abbexa | Hitachi 7170; R1:40ml×2 R2:20ml×1 | EUR 692 |
Lipase Assay Kit |
|||
abx098442-Hitachi7170R160ml1R215ml1 | Abbexa | Hitachi 7170; R1:60ml×1 R2:15ml×1 | EUR 566 |
Lipase Assay Kit |
|||
abx098442-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1:40ml×1 R2:10ml×1 | EUR 441 |
Lipase Assay Kit |
|||
abx098442-UniversalR120ml1R25ml1 | Abbexa | Universal; R1:20ml×1 R2:5ml×1 | EUR 316 |
Magnesium Assay Kit |
|||
abx098445-Hitachi7020R140ml2R240ml2 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
Magnesium Assay Kit |
|||
abx098445-Hitachi7060R190ml1R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×1 R2: 90ml×1 | EUR 206 |
Magnesium Assay Kit |
|||
abx098445-Toshiba40R140ml2R240ml2 | Abbexa | Toshiba 40; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
Magnesium Assay Kit |
|||
abx098445-Toshiba40R150ml2R250ml2 | Abbexa | Toshiba 40; R1: 50ml×2 R2: 50ml×2 | EUR 206 |
Microalbumin Assay KIt |
|||
abx098446-Hitachi7060R150ml1R210ml1 | Abbexa | Hitachi 7060; R1: 50ml×1 R2: 10ml×1 | EUR 363 |
Microalbumin Assay KIt |
|||
abx098446-Hitachi7060R150ml3R230ml1 | Abbexa | Hitachi 7060; R1: 50ml×3 R2: 30ml×1 | EUR 363 |
Microalbumin Assay KIt |
|||
abx098446-Hitachi7170R125ml1R25ml1 | Abbexa | Hitachi 7170; R1: 25ml×1 R2: 5ml×1 | EUR 269 |
Microalbumin Assay KIt |
|||
abx098446-Hitachi7170R150ml1R210ml1 | Abbexa | Hitachi 7170; R1: 50ml×1 R2: 10ml×1 | EUR 363 |
Myoglobin Assay Kit |
|||
abx098448-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1: 40ml×1 R2: 10ml×1 | EUR 1193 |
Myoglobin Assay Kit |
|||
abx098448-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 692 |
Myoglobin Assay Kit |
|||
abx098448-Hitachi7170R140ml1R210ml1 | Abbexa | Hitachi 7170; R1: 40ml×1 R2: 10ml×1 | EUR 1193 |