Comparison of diagnostic accuracies of rapid serological tests and ELISA

September 8, 2020

Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospital

Objectives: To assess the diagnostic performance of rapid lateral flow immunochromatographic assays (LFAs) compared with an ELISA and nucleic acid amplification tests (NATs) in individuals with suspected coronavirus disease 2019 (COVID-19).
Methods: Patients presenting to a Dutch teaching hospital were eligible between 17 March and 10 April 2020, when they had respiratory symptoms that were suspected for COVID-19. The performances of six different LFAs were evaluated in plasma samples obtained on corresponding respiratory sample dates of NATs testing. Subsequently, the best performing LFA was evaluated in 228 patients and in 50 sera of a historical patient control group.
Results: In the pilot analysis, sensitivity characteristics of LFA were heterogeneous, ranging from 2/20 (10%; 95% CI 0%-23%) to 11/20 (55%; 95% CI 33%-77%). In the total cohort, Orient Gene Biotech COVID-19 IgG/IgM Rapid Test LFA had a sensitivity of 43/99 (43%; 95% CI 34%-53%) and specificity of 126/129 (98%; 95% CI 95%-100%).
Sensitivity increased to 31/52 (60%; 95% CI 46%-73%) in patients with at least 7 days of symptoms, and to 21/33 (64%; 95% CI 47%-80%) in patients with C-reactive protein (CRP) ≥100 mg/L. Sensitivity and specificity of Wantai SARS-CoV-2 Ab ELISA was 59/95 (62%; 95% CI 52%-72%) and 125/128 (98%; 95% CI 95%-100%) in all patients, respectively, but sensitivity increased to 38/48 (79%; 95% CI 68%-91%) in patients with at least 7 days of symptoms.
Conclusions: There is large variability in diagnostic test performance between rapid LFAs, but overall limited sensitivity and high specificity in acutely admitted patients. Sensitivity improved in patients with longer existing symptoms or high CRP. LFAs should only be considered as additional triage tools when these may lead to the improvement of hospital logistics.
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Adiponectin (rat) Elisa Assay Kit
GWB-AXR241 1 x 96 assays Ask for price

Adiponectin (rat) Elisa Assay Kit
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Adiponectin (human) Elisa Assay Kit
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Adiponectin (mouse) Elisa Assay Kit
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Adiponectin (human) ELISA Assay Kit
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Adiponectin (mouse) Elisa Assay Kit
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Adiponectin (human) Elisa Assay Kit
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Adiponectin (mouse) Elisa Assay Kit
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Ultra Nuclease Assay Kit(ELISA)
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Feline SAA Detection ELISA Assay Kit
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Elite cAMP ELISA Assay Kit (Fluorescence)
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Ultra Nuclease Assay Kit 2.0(ELISA)
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Cas9 ELISA Assay
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K2/Spice Synthetic Cannabinoids ELISA Assay Kit
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OVALBUMIN SPECIFIC IgE ELISA ASSAY KIT (OKSA11256)
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Screen Questâ„¢ Colorimetric ELISA cAMP Assay Kit
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Screen Questâ„¢ Fluorimetric ELISA cAMP Assay Kit
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Elite cAMP 384-Well ELISA Assay Kit (Fluorescence)
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Elite cAMP 384-Well ELISA Assay Kit (Fluorescence)
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Elite gp120 (HIV-1/Clade B) ELISA Assay Kit
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Elite gp120 (HIV-1/Clade B) ELISA Assay Kit
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Rat uPA total antigen assay ELISA Kit
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Rat uPA total antigen assay ELISA Kit
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Rat tPA total antigen assay ELISA Kit
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Rat tPA total antigen assay ELISA Kit
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ELISA Assay Buffer, 20x
GR103001 1 L
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ELISA Assay Buffer, 80x
GR103002 1 L
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Mouse uPA total antigen assay ELISA Kit
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Mouse uPA total antigen assay ELISA Kit
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Human tPA total antigen assay ELISA Kit
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Human tPA total antigen assay ELISA Kit
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Human uPA total antigen assay ELISA Kit
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Human uPA total antigen assay ELISA Kit
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Mouse tPA total antigen assay ELISA Kit
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Active rat tPA functional assay ELISA Kit
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Active rat uPA functional assay ELISA Kit
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Active rat uPA functional assay ELISA Kit
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Rat Fibrinogen ELISA assay
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Rat Fibrinogen ELISA assay
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Creatinine Assay Kit (urine normalizing assay)
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Creatinine Assay Kit (urine normalizing assay)
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Active human uPA functional assay ELISA Kit
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Rat fibrinogen total antigen assay ELISA Kit
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Human Prothrombin total antigen assay ELISA Kit
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Mouse Prothrombin total antigen assay ELISA Kit
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Human vitronectin total antigen assay ELISA Kit
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EpiQuik CRISPR/Cas9 Assay ELISA Kit (Colorimetric)
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Porcine fibrinogen total antigen assay ELISA Kit
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ELISA GENERAL ASSAY DILUENT
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ELISA GENERAL ASSAY DILUENT
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ELISA GENERAL ASSAY DILUENT
MBS239227-1L 1L
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ELISA NEPTUNE ASSAY DILUENT
MBS239230-500mL 500mL
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ADA Assay Kit
abx098403-Hitachi7060R190ml2R290ml1 Hitachi 7060; R1: 90ml×2 R2: 90ml×1
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abx098403-Hitachi7170R140ml4R220ml4 Hitachi 7170; R1: 40ml×4 R2: 20ml×4
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ADA Assay Kit
abx098403-Hitachi7170R160ml4R260ml2 Hitachi 7170; R1: 60ml×4 R2: 60ml×2
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ADA Assay Kit
abx098403-Toshiba40R150ml4R250ml2 Toshiba 40; R1: 50ml×4 R2: 50ml×2
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Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients

Objectives: To evaluate the diagnostic performance of seven rapid IgG/IgM tests and the Euroimmun IgA/IgG ELISA for antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in COVID-19 patients.
Methods: Specificity was evaluated in 103 samples collected before January 2020. Sensitivity and time to seropositivity was evaluated in 167 samples from 94 patients with COVID-19 confirmed with RT-PCR on nasopharyngeal swab.
Results: Specificity (confidence interval) of lateral flow assays (LFAs) was ≥91.3% (84.0-95.5) for IgM, ≥90.3% (82.9-94.8) for IgG, and ≥85.4% (77.2-91.1) for the combination IgM OR IgG. Specificity of the ELISA was 96.1% (90.1-98.8) for IgG and only 73.8% (64.5-81.4) for IgA. Sensitivity 14-25 days after the onset of symptoms was between ≥92.1% (78.5-98.0) and 100% (95.7-100) for IgG LFA compared to 89.5% (75.3-96.4) for IgG ELISA. Positivity of IgM OR IgG for LFA resulted in a decrease in specificity compared to IgG alone without a gain in diagnostic performance, except for VivaDiag.
The results for IgM varied significantly between the LFAs with an average overall agreement of only 70% compared to 89% for IgG. The average dynamic trend to seropositivity for IgM was not shorter than for IgG. At the time of hospital admission the sensitivity of LFA was <60%.
Conclusions: Sensitivity for the detection of IgG antibodies 14-25 days after the onset of symptoms was ≥92.1% for all seven LFAs compared to 89.5% for the IgG ELISA. The results for IgM varied significantly, and including IgM antibodies in addition to IgG for the interpretation of LFAs did not improve the diagnostic performance.

Association of Genetic Polymorphisms Related to Johne’s Disease With Estimated Breeding Values of Holstein Sires for Milk ELISATest Scores

Background: Johne’s disease (JD) is a chronic intestinal inflammatory disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection in ruminants. Since there are currently no effective vaccine or treatment options available to control JD, genetic selection may be an alternative strategy to enhance JD resistance.
Numerous Single Nucleotide Polymorphisms (SNPs) have been reported to be associated with MAP infection status based on published genome-wide association and candidate gene studies. The main objective of this study was to validate these SNPs that were previously identified to be associated with JD by testing their effect on Holstein bulls’ estimated breeding values (EBVs) for milk ELISA test scores, an indirect indicator of MAP infection status in cattle.
Results: Three SNPs, rs41810662, rs41617133 and rs110225854, located on Bos taurus autosomes (BTA) 16, 23 and 26, respectively, were confirmed as significantly associated with Holstein bulls’ EBVs for milk ELISA test score (FDR < 0.01) based on General Quasi Likelihood Scoring analysis (GQLS) analysis. Single-SNP regression analysis identified four SNPs that were associated with sire EBVs (FDR < 0.05).
This includes two SNPs that were common with GQLS (rs41810662 and rs41617133), with the other two SNPs being rs110494981 and rs136182707, located on BTA9 and BTA16, respectively.
Conclusions: The findings of this study validate the association of SNPs with JD MAP infection status and highlight the need to further investigate the genomic regions harboring these SNPs.

ICK (Ab-159) Antibody
CSB-PA153658- each
EUR 402
Description: A polyclonal antibody against ICK (Ab-159). Recognizes ICK (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ICK (Ab-159) Antibody
CSB-PA153658-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against ICK (Ab-159). Recognizes ICK (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

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EUR 270

ICK (Ab-159) Antibody
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ICK (Ab-159) Antibody
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EUR 305

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MBS851572-01mLAF405L 0.1mL(AF405L)
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ICK (Ab-159) Antibody
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ICK (Ab-159) Antibody
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ICK (Ab-159) Conjugated Antibody
C33228 100ul
EUR 476.4

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MBS9444252-01mLAF350 0.1mL(AF350)
EUR 480

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12403 100ug
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Anti-DNA Antibody
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Anti-DNA Antibody
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Anti-DNA Antibody
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12404 100ug
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Anti-DNA Antibody
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Anti-DNA Antibody
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Anti-ATM Antibody
13012 100ug
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2025 100ug
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Anti-CCR8 Antibody
2097 100ug
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Anti-IKKα Antibody
2115 100ug
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Anti-IKKα Antibody
2117 100ug
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Anti-F1Aa Antibody
2279 100ug
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2283 100ug
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23004 100ug
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2419 100ug
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28105 100ug
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28117 100ug
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28119 100ug
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3338 100ug
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34012 100ug
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34016 100ug
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34036 100ug
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34044 100ug
EUR 372