Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospital
Objectives: To assess the diagnostic performance of rapid lateral flow immunochromatographic assays (LFAs) compared with an ELISA and nucleic acid amplification tests (NATs) in individuals with suspected coronavirus disease 2019 (COVID-19).
Methods: Patients presenting to a Dutch teaching hospital were eligible between 17 March and 10 April 2020, when they had respiratory symptoms that were suspected for COVID-19. The performances of six different LFAs were evaluated in plasma samples obtained on corresponding respiratory sample dates of NATs testing. Subsequently, the best performing LFA was evaluated in 228 patients and in 50 sera of a historical patient control group.
Results: In the pilot analysis, sensitivity characteristics of LFA were heterogeneous, ranging from 2/20 (10%; 95% CI 0%-23%) to 11/20 (55%; 95% CI 33%-77%). In the total cohort, Orient Gene Biotech COVID-19 IgG/IgM Rapid Test LFA had a sensitivity of 43/99 (43%; 95% CI 34%-53%) and specificity of 126/129 (98%; 95% CI 95%-100%).
Sensitivity increased to 31/52 (60%; 95% CI 46%-73%) in patients with at least 7 days of symptoms, and to 21/33 (64%; 95% CI 47%-80%) in patients with C-reactive protein (CRP) ≥100 mg/L. Sensitivity and specificity of Wantai SARS-CoV-2 Ab ELISA was 59/95 (62%; 95% CI 52%-72%) and 125/128 (98%; 95% CI 95%-100%) in all patients, respectively, but sensitivity increased to 38/48 (79%; 95% CI 68%-91%) in patients with at least 7 days of symptoms.
Conclusions: There is large variability in diagnostic test performance between rapid LFAs, but overall limited sensitivity and high specificity in acutely admitted patients. Sensitivity improved in patients with longer existing symptoms or high CRP. LFAs should only be considered as additional triage tools when these may lead to the improvement of hospital logistics.
 Frit Kit |
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| FRIT-KIT | Next Advance | 1each | EUR 124.00 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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 Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1035.00 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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 PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | EUR 266.00 |
 ELISA Assay Kit) Adiponectin (human) ELISA Assay Kit |
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| K4901-100 | Biovision | EUR 898.00 | |
 Elisa Assay Kit) Adiponectin (mouse) Elisa Assay Kit |
|||
| K4902-100 | Biovision | EUR 865.00 | |
 Elisa Assay Kit) Adiponectin (rat) Elisa Assay Kit |
|||
| K4903-100 | Biovision | EUR 843.00 | |
 Elisa Assay Kit) Visfatin (human) Elisa Assay Kit |
|||
| K4907-100 | Biovision | EUR 985.00 | |
 Elisa Assay Kit) Adiponectin (human) Elisa Assay Kit |
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| K2192-96 | ApexBio | 96 assays | EUR 1017.00 |
Adiponectin (mouse) Elisa Assay Kit |
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| K2193-96 | ApexBio | 96 assays | EUR 1017.00 |
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110.00 |
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 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | EUR 1132.00 |
|
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 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | EUR 1132.00 |
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 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132.00 |
|
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 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV125PA-KIT | SBI | 1 Kit | EUR 1132.00 |
|
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 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | EUR 1132.00 |
|
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 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | EUR 1132.00 |
|
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 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | EUR 1132.00 |
|
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) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | EUR 805.00 |
|
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 Glycosaminoglycans Assay Kit |
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| 6022 | Chondrex | 1 kit | EUR 419.75 |
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Description: Glycosaminoglycans Assay Kit |
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 DNA Assay Kit |
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| 6023 | Chondrex | 1 kit | EUR 180.50 |
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Description: DNA Assay Kit |
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 Hemoglobin Assay Kit |
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| 6024 | Chondrex | 1 kit | EUR 180.50 |
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Description: Hemoglobin Assay Kit |
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 Proteasome Assay Kit |
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| 55R-1341 | Fitzgerald | 100 assays | EUR 654.00 |
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Description: Assay Kit for detection of Proteasome in the research laboratory |
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 Calpain Assay Kit |
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| 55R-1342 | Fitzgerald | 100 assays | EUR 779.00 |
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Description: Assay Kit for detection of Calpain in the research laboratory |
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 Glutathione Assay Kit |
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| 55R-1343 | Fitzgerald | 100 assays | EUR 654.00 |
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Description: Assay Kit for detection of Glutathione in the research laboratory |
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Glutathione Assay Kit |
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| 55R-1354 | Fitzgerald | 100 assays | EUR 895.00 |
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Description: Assay Kit for detection of Glutathione activity in the research laboratory |
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 HDAC Assay Kit |
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| 55R-1371 | Fitzgerald | 100 assays | EUR 673.00 |
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Description: Assay Kit for detection of HDAC in the research laboratory |
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HDAC Assay Kit |
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| 55R-1372 | Fitzgerald | 100 assays | EUR 673.00 |
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Description: Assay Kit for detection of HDAC in the research laboratory |
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 HAT Assay Kit |
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| 55R-1373 | Fitzgerald | 100 assays | EUR 740.00 |
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Description: Assay Kit for detection of HAT in the research laboratory |
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 SOD Assay Kit |
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| 55R-1374 | Fitzgerald | 100 assays | EUR 602.00 |
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Description: Assay Kit for detection of SOD in the research laboratory |
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 HDAC3 Assay Kit |
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| 55R-1377 | Fitzgerald | 100 assays | EUR 693.00 |
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Description: Assay Kit for detection of HDAC3 in the research laboratory |
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 HDAC8 Assay Kit |
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| 55R-1379 | Fitzgerald | 100 assays | EUR 689.00 |
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Description: Assay Kit for detection of HDAC8 in the research laboratory |
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 ATP Assay Kit |
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| 55R-1380 | Fitzgerald | 100 assays | EUR 809.00 |
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Description: Assay Kit for detection of ATP in the research laboratory |
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 ADP Assay Kit |
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| 55R-1381 | Fitzgerald | 100 assays | EUR 809.00 |
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Description: Assay Kit for detection of ADP in the research laboratory |
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 FAD Assay Kit |
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| 55R-1382 | Fitzgerald | 100 assays | EUR 732.00 |
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Description: Assay Kit for detection of FAD in the research laboratory |
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 PEP Assay Kit |
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| 55R-1384 | Fitzgerald | 100 assays | EUR 876.00 |
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Description: Assay Kit for detection of PEP in the research laboratory |
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 Ammonia Assay Kit |
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| 55R-1388 | Fitzgerald | 100 assays | EUR 740.00 |
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Description: Assay Kit for detection of Ammonia in the research laboratory |
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 cAMP Assay Kit |
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| 55R-1389 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of cAMP activity in the research laboratory |
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 cGMP Assay Kit |
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| 55R-1390 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of cGMP activity in the research laboratory |
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 Urea Assay Kit |
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| 55R-1391 | Fitzgerald | 100 assays | EUR 740.00 |
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Description: Assay Kit for detection of Urea in the research laboratory |
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 Calcium Assay Kit |
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| 55R-1392 | Fitzgerald | 250 assays | EUR 586.00 |
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Description: Assay Kit for detection of Calcium activity in the research laboratory |
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 Magnesium Assay Kit |
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| 55R-1393 | Fitzgerald | 100 assays | EUR 654.00 |
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Description: Assay Kit for detection of Magnesium in the research laboratory |
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 Zinc Assay Kit |
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| 55R-1394 | Fitzgerald | 100 assays | EUR 673.00 |
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Description: Assay Kit for detection of Zinc in the research laboratory |
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 Iron Assay Kit |
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| 55R-1395 | Fitzgerald | 100 assays | EUR 758.00 |
|
Description: Assay Kit for detection of Iron in the research laboratory |
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 Phosphate Assay Kit |
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| 55R-1400 | Fitzgerald | 500 assays | EUR 415.00 |
|
Description: Assay Kit for detection of Phosphate in the research laboratory |
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 JNK Assay Kit |
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| 55R-1407 | Fitzgerald | 40 assays | EUR 868.00 |
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Description: Assay Kit for detection of JNK in the research laboratory |
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JNK Assay Kit |
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| 55R-1408 | Fitzgerald | 40 assays | EUR 979.00 |
|
Description: Assay Kit for detection of JNK in the research laboratory |
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 Akt Assay Kit |
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| 55R-1409 | Fitzgerald | 40 assays | EUR 979.00 |
|
Description: Assay Kit for detection of Akt in the research laboratory |
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Ammonia Assay Kit |
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| 55R-1410 | Fitzgerald | 100 assays | EUR 740.00 |
|
Description: Assay Kit for detection of Ammonia in the research laboratory |
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 Cobalt Assay Kit |
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| 55R-1435 | Fitzgerald | 100 assays | EUR 621.00 |
|
Description: Assay Kit for detection of Cobalt in the research laboratory |
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 Nickel Assay Kit |
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| 55R-1436 | Fitzgerald | 100 assays | EUR 621.00 |
|
Description: Assay Kit for detection of Nickel in the research laboratory |
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 Chloride Assay Kit |
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| 55R-1437 | Fitzgerald | 100 assays | EUR 484.00 |
|
Description: Assay Kit for detection of Chloride in the research laboratory |
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 Aspartate Assay Kit |
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| 55R-1438 | Fitzgerald | 100 assays | EUR 673.00 |
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Description: Assay Kit for detection of Aspartate in the research laboratory |
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 Hydroxyproline Assay Kit |
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| 55R-1439 | Fitzgerald | 100 assays | EUR 844.00 |
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Description: Assay Kit for detection of Hydroxyproline in the research laboratory |
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 Phenylalanine Assay Kit |
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| 55R-1442 | Fitzgerald | 100 assays | EUR 654.00 |
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Description: Assay Kit for detection of Phenylalanine in the research laboratory |
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 Phosphatidylcholine Assay Kit |
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| 55R-1443 | Fitzgerald | 100 assays | EUR 673.00 |
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Description: Assay Kit for detection of Phosphatidylcholine in the research laboratory |
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 Glucose Assay Kit |
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| 55R-1449 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of Glucose in the research laboratory |
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 Lactate Assay Kit |
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| 55R-1450 | Fitzgerald | 100 assays | EUR 844.00 |
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Description: Assay Kit for detection of Lactate in the research laboratory |
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 Pyruvate Assay Kit |
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| 55R-1452 | Fitzgerald | 100 assays | EUR 844.00 |
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Description: Assay Kit for detection of Pyruvate in the research laboratory |
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 Adipogenesis Assay Kit |
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| 55R-1454 | Fitzgerald | 100 assays | EUR 586.00 |
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Description: Assay Kit for detection of Adipogenesis in the research laboratory |
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Glucose Assay Kit |
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| 55R-1458 | Fitzgerald | 100 assays | EUR 740.00 |
|
Description: Assay Kit for detection of Glucose in the research laboratory |
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 Galactose Assay Kit |
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| 55R-1459 | Fitzgerald | 100 assays | EUR 740.00 |
|
Description: Assay Kit for detection of Galactose in the research laboratory |
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 Maltose Assay Kit |
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| 55R-1460 | Fitzgerald | 100 assays | EUR 723.00 |
|
Description: Assay Kit for detection of Maltose in the research laboratory |
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 Fructose Assay Kit |
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| 55R-1461 | Fitzgerald | 100 assays | EUR 689.00 |
|
Description: Assay Kit for detection of Fructose in the research laboratory |
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 Ethanol Assay Kit |
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| 55R-1462 | Fitzgerald | 100 assays | EUR 774.00 |
|
Description: Assay Kit for detection of Ethanol in the research laboratory |
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Galactose Assay Kit |
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| 55R-1463 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of Galactose in the research laboratory |
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 Lactose Assay Kit |
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| 55R-1466 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of Lactose in the research laboratory |
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 Creatinine Assay Kit |
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| 55R-1467 | Fitzgerald | 100 assays | EUR 638.00 |
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Description: Assay Kit for detection of Creatinine in the research laboratory |
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 Sucrose Assay Kit |
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| 55R-1468 | Fitzgerald | 100 assays | EUR 689.00 |
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Description: Assay Kit for detection of Sucrose in the research laboratory |
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Lactate Assay Kit |
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| 55R-1469 | Fitzgerald | 100 assays | EUR 844.00 |
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Description: Assay Kit for detection of Lactate in the research laboratory |
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Maltose Assay Kit |
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| 55R-1470 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of Maltose in the research laboratory |
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 Glutamate Assay Kit |
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| 55R-1471 | Fitzgerald | 100 assays | EUR 740.00 |
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Description: Assay Kit for detection of Glutamate in the research laboratory |
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 Fumarate Assay Kit |
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| 55R-1475 | Fitzgerald | 100 assays | EUR 706.00 |
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Description: Assay Kit for detection of Fumarate in the research laboratory |
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 Creatine Assay Kit |
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| 55R-1476 | Fitzgerald | 100 assays | EUR 654.00 |
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Description: Assay Kit for detection of Creatine in the research laboratory |
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 Sarcosine Assay Kit |
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| 55R-1477 | Fitzgerald | 100 assays | EUR 654.00 |
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Description: Assay Kit for detection of Sarcosine in the research laboratory |
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 Malate Assay Kit |
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| 55R-1478 | Fitzgerald | 100 assays | EUR 673.00 |
|
Description: Assay Kit for detection of Malate in the research laboratory |
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 Glycogen Assay Kit |
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| 55R-1481 | Fitzgerald | 100 assays | EUR 844.00 |
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Description: Assay Kit for detection of Glycogen in the research laboratory |
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 Starch Assay Kit |
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| 55R-1482 | Fitzgerald | 100 assays | EUR 673.00 |
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Description: Assay Kit for detection of Starch in the research laboratory |
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 Alanine Assay Kit |
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| 55R-1483 | Fitzgerald | 100 assays | EUR 654.00 |
|
Description: Assay Kit for detection of Alanine in the research laboratory |
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 Formate Assay Kit |
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| 55R-1484 | Fitzgerald | 100 assays | EUR 809.00 |
|
Description: Assay Kit for detection of Formate in the research laboratory |
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 Citrate Assay Kit |
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| 55R-1485 | Fitzgerald | 100 assays | EUR 740.00 |
|
Description: Assay Kit for detection of Citrate in the research laboratory |
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 Isocitrate Assay Kit |
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| 55R-1486 | Fitzgerald | 100 assays | EUR 663.00 |
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Description: Assay Kit for detection of Isocitrate in the research laboratory |
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 Oxaloacetate Assay Kit |
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| 55R-1488 | Fitzgerald | 100 assays | EUR 663.00 |
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Description: Assay Kit for detection of Oxaloacetate in the research laboratory |
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 Heme Assay Kit |
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| 55R-1492 | Fitzgerald | 100 assays | EUR 673.00 |
|
Description: Assay Kit for detection of Heme in the research laboratory |
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Glucose Assay Kit |
|||
| 55R-1494 | Fitzgerald | 100 assays | EUR 706.00 |
|
Description: Assay Kit for detection of Glucose in the research laboratory |
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 Amylase Assay Kit |
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| 55R-1503 | Fitzgerald | 100 assays | EUR 551.00 |
|
Description: Assay Kit for detection of Amylase in the research laboratory |
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 Inosine Assay Kit |
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| 55R-1504 | Fitzgerald | 100 assays | EUR 732.00 |
|
Description: Assay Kit for detection of Inosine in the research laboratory |
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 Aconitase Assay Kit |
|||
| 55R-1505 | Fitzgerald | 100 assays | EUR 689.00 |
|
Description: Assay Kit for detection of Aconitase in the research laboratory |
|||
 Lipase Assay Kit |
|||
| 55R-1506 | Fitzgerald | 100 assays | EUR 706.00 |
|
Description: Assay Kit for detection of Lipase in the research laboratory |
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Lipase Assay Kit |
|||
| 55R-1507 | Fitzgerald | 100 assays | EUR 740.00 |
|
Description: Assay Kit for detection of Lipase in the research laboratory |
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 MDA Assay Kit |
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| 55R-1512 | Fitzgerald | 100 assays | EUR 809.00 |
|
Description: Assay Kit for detection of MDA in the research laboratory |
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 Myeloperoxidase Assay Kit |
|||
| 55R-1514 | Fitzgerald | 100 assays | EUR 809.00 |
|
Description: Assay Kit for detection of Myeloperoxidase in the research laboratory |
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Myeloperoxidase Assay Kit |
|||
| 55R-1515 | Fitzgerald | 100 assays | EUR 758.00 |
|
Description: Assay Kit for detection of Myeloperoxidase in the research laboratory |
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 Asparaginase Assay Kit |
|||
| 55R-1518 | Fitzgerald | 100 assays | EUR 758.00 |
|
Description: Assay Kit for detection of Asparaginase in the research laboratory |
|||
 Trypsin Assay Kit |
|||
| 55R-1524 | Fitzgerald | 100 assays | EUR 621.00 |
|
Description: Assay Kit for detection of Trypsin in the research laboratory |
|||
 Catalase Assay Kit |
|||
| 55R-1525 | Fitzgerald | 100 assays | EUR 570.00 |
|
Description: Assay Kit for detection of Catalase in the research laboratory |
|||
 DPP4 Assay Kit |
|||
| 55R-1526 | Fitzgerald | 100 assays | EUR 750.00 |
|
Description: Assay Kit for detection of DPP4 in the research laboratory |
|||
 Protease Assay Kit |
|||
| 55R-1528 | Fitzgerald | 100 assays | EUR 519.00 |
|
Description: Assay Kit for detection of Protease in the research laboratory |
|||
 MMP3 Assay Kit |
|||
| 55R-1530 | Fitzgerald | 100 assays | EUR 723.00 |
|
Description: Assay Kit for detection of MMP3 in the research laboratory |
|||
 PAH Assay Kit |
|||
| 55R-1547 | Fitzgerald | 100 assays | EUR 740.00 |
|
Description: Assay Kit for detection of PAH in the research laboratory |
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Pyruvate Assay Kit |
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| abx097982-100Assays | Abbexa | 100 Assays | EUR 472.00 |
|
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 NADPase Assay Kit |
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| abx097983-100Assays | Abbexa | 100 Assays | EUR 504.00 |
|
|||
Starch Assay Kit |
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| abx097988-100Assays | Abbexa | 100 Assays | EUR 441.00 |
|
|||
 Trehalose Assay Kit |
|||
| abx097995-100Assays | Abbexa | 100 Assays | EUR 472.00 |
|
|||
 ADA Assay Kit |
|||
| abx098403-Hitachi7060R190ml2R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 90ml×1 | EUR 739.00 |
|
|||
ADA Assay Kit |
|||
| abx098403-Hitachi7170R140ml4R220ml4 | Abbexa | Hitachi 7170; R1: 40ml×4 R2: 20ml×4 | EUR 801.00 |
|
|||
ADA Assay Kit |
|||
| abx098403-Hitachi7170R160ml4R260ml2 | Abbexa | Hitachi 7170; R1: 60ml×4 R2: 60ml×2 | EUR 911.00 |
|
|||
ADA Assay Kit |
|||
| abx098403-Toshiba40R150ml4R250ml2 | Abbexa | Toshiba 40; R1: 50ml×4 R2: 50ml×2 | EUR 786.00 |
|
|||
Calcium Assay Kit |
|||
| abx098414-Hitachi7020R140ml2R240ml2 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 40ml×2 | EUR 206.00 |
|
|||
Calcium Assay Kit |
|||
| abx098414-Hitachi7060R190ml1R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×1 R2: 90ml×1 | EUR 206.00 |
|
|||
Calcium Assay Kit |
|||
| abx098414-Toshiba40R140ml2R240ml2 | Abbexa | Toshiba 40; R1: 40ml×2 R2: 40ml×2 | EUR 206.00 |
|
|||
Calcium Assay Kit |
|||
| abx098414-UniversalR140ml2R240ml2 | Abbexa | Universal; R1: 40ml×2 R2: 40ml×2 | EUR 206.00 |
|
|||
 Cholinesterase Assay Kit |
|||
| abx098416-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 300.00 |
|
|||
Cholinesterase Assay Kit |
|||
| abx098416-Hitachi7170R140ml3R230ml1 | Abbexa | Hitachi 7170; R1: 40ml×3 R2: 30ml×1 | EUR 316.00 |
|
|||
Cholinesterase Assay Kit |
|||
| abx098416-Hitachi7170R160ml2R230ml1 | Abbexa | Hitachi 7170; R1: 60ml×2 R2: 30ml×1 | EUR 316.00 |
|
|||
Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients
Objectives: To evaluate the diagnostic performance of seven rapid IgG/IgM tests and the Euroimmun IgA/IgG ELISA for antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in COVID-19 patients.
Methods: Specificity was evaluated in 103 samples collected before January 2020. Sensitivity and time to seropositivity was evaluated in 167 samples from 94 patients with COVID-19 confirmed with RT-PCR on nasopharyngeal swab.
Results: Specificity (confidence interval) of lateral flow assays (LFAs) was ≥91.3% (84.0-95.5) for IgM, ≥90.3% (82.9-94.8) for IgG, and ≥85.4% (77.2-91.1) for the combination IgM OR IgG. Specificity of the ELISA was 96.1% (90.1-98.8) for IgG and only 73.8% (64.5-81.4) for IgA. Sensitivity 14-25 days after the onset of symptoms was between ≥92.1% (78.5-98.0) and 100% (95.7-100) for IgG LFA compared to 89.5% (75.3-96.4) for IgG ELISA. Positivity of IgM OR IgG for LFA resulted in a decrease in specificity compared to IgG alone without a gain in diagnostic performance, except for VivaDiag.
The results for IgM varied significantly between the LFAs with an average overall agreement of only 70% compared to 89% for IgG. The average dynamic trend to seropositivity for IgM was not shorter than for IgG. At the time of hospital admission the sensitivity of LFA was <60%.
Conclusions: Sensitivity for the detection of IgG antibodies 14-25 days after the onset of symptoms was ≥92.1% for all seven LFAs compared to 89.5% for the IgG ELISA. The results for IgM varied significantly, and including IgM antibodies in addition to IgG for the interpretation of LFAs did not improve the diagnostic performance.
Association of Genetic Polymorphisms Related to Johne’s Disease With Estimated Breeding Values of Holstein Sires for Milk ELISATest Scores
Background: Johne’s disease (JD) is a chronic intestinal inflammatory disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection in ruminants. Since there are currently no effective vaccine or treatment options available to control JD, genetic selection may be an alternative strategy to enhance JD resistance.
Numerous Single Nucleotide Polymorphisms (SNPs) have been reported to be associated with MAP infection status based on published genome-wide association and candidate gene studies. The main objective of this study was to validate these SNPs that were previously identified to be associated with JD by testing their effect on Holstein bulls’ estimated breeding values (EBVs) for milk ELISA test scores, an indirect indicator of MAP infection status in cattle.
Results: Three SNPs, rs41810662, rs41617133 and rs110225854, located on Bos taurus autosomes (BTA) 16, 23 and 26, respectively, were confirmed as significantly associated with Holstein bulls’ EBVs for milk ELISA test score (FDR < 0.01) based on General Quasi Likelihood Scoring analysis (GQLS) analysis. Single-SNP regression analysis identified four SNPs that were associated with sire EBVs (FDR < 0.05).
This includes two SNPs that were common with GQLS (rs41810662 and rs41617133), with the other two SNPs being rs110494981 and rs136182707, located on BTA9 and BTA16, respectively.
Conclusions: The findings of this study validate the association of SNPs with JD MAP infection status and highlight the need to further investigate the genomic regions harboring these SNPs.
 Antibody) ICK (Ab-159) Antibody |
|||
| CSB-PA153658-100ul | Cusabio | 100ul | EUR 316.00 |
|
|||
|
Description: A polyclonal antibody against ICK (Ab-159). Recognizes ICK (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
|||
 Conjugated Antibody) ICK (Ab-159) Conjugated Antibody |
|||
| C33228 | SAB | 100ul | EUR 397.00 |
 Antibody) Anti-GAB2 (Ab-159) Antibody |
|||
| A02386-2 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal GAB2 (Ab-159) Antibody. Validated in IHC, WB and tested in Human. |
|||
 Antibody) MAK (Ab-159) Antibody |
|||
| 33233-100ul | SAB | 100ul | EUR 252.00 |
MAK (Ab-159) Antibody |
|||
| 33233-50ul | SAB | 50ul | EUR 187.00 |
 Antibody) GAB2 (Ab-159) Antibody |
|||
| 33262-100ul | SAB | 100ul | EUR 252.00 |
GAB2 (Ab-159) Antibody |
|||
| 33262-50ul | SAB | 50ul | EUR 187.00 |
MAK (Ab-159) Antibody |
|||
| CSB-PA056397- | Cusabio | EUR 335.00 | |
|
|||
|
Description: A polyclonal antibody against MAK (Ab-159). Recognizes MAK (Ab-159) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
|||
MAK (Ab-159) Antibody |
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| CSB-PA056397-100ul | Cusabio | 100ul | EUR 316.00 |
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Description: A polyclonal antibody against MAK (Ab-159). Recognizes MAK (Ab-159) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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GAB2 (Ab-159) Antibody |
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| CSB-PA781252- | Cusabio | EUR 335.00 | |
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Description: A polyclonal antibody against GAB2 (Ab-159). Recognizes GAB2 (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
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GAB2 (Ab-159) Antibody |
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| CSB-PA781252-100ul | Cusabio | 100ul | EUR 316.00 |
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Description: A polyclonal antibody against GAB2 (Ab-159). Recognizes GAB2 (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
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 Conjugated Antibody) MAK (Ab-159) Conjugated Antibody |
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| C33233 | SAB | 100ul | EUR 397.00 |
 Conjugated Antibody) GAB2 (Ab-159) Conjugated Antibody |
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| C33262 | SAB | 100ul | EUR 397.00 |
 Anti-ICK Antibody |
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| A1172-100 | Biovision | EUR 370.00 | |
Anti-ICK Antibody |
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| A06270 | BosterBio | 100ul | EUR 397.00 |
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Description: Rabbit Polyclonal ICK Antibody. Validated in IF, IHC and tested in Human, Mouse. |
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 Anti-ICK antibody |
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| PAab04108 | Lifescience Market | 100 ug | EUR 386.00 |
Anti-ICK antibody |
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| STJ110093 | St John's Laboratory | 100 µl | EUR 277.00 |
|
Description: Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. |
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Anti-ICK antibody |
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| STJ93636 | St John's Laboratory | 200 µl | EUR 197.00 |
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Description: Rabbit polyclonal to ICK. |
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 anti-ICK |
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| YF-PA17639 | Abfrontier | 100 ug | EUR 403.00 |
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Description: Rabbit polyclonal to ICK |
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anti-ICK |
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| YF-PA25803 | Abfrontier | 50 ul | EUR 334.00 |
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Description: Mouse polyclonal to ICK |
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 Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 349.00 |
 ICK antibody |
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| 70R-17871 | Fitzgerald | 50 ul | EUR 435.00 |
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Description: Rabbit polyclonal ICK antibody |
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ICK antibody |
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| 70R-32214 | Fitzgerald | 100 ug | EUR 327.00 |
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Description: Rabbit polyclonal ICK antibody |
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 ICK Antibody |
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| 34122-100ul | SAB | 100ul | EUR 252.00 |
ICK Antibody |
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| 34122-50ul | SAB | 50ul | EUR 187.00 |
ICK Antibody |
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| 42899-100ul | SAB | 100ul | EUR 252.00 |
ICK Antibody |
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| 1-CSB-PA891800LA01HU | Cusabio |
|
|
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
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ICK Antibody |
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| CSB-PA278117- | Cusabio | EUR 335.00 | |
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500 |
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ICK Antibody |
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| CSB-PA278117-100ul | Cusabio | 100ul | EUR 316.00 |
|
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|
Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500 |
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ICK antibody |
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| 70R-32652 | Fitzgerald | 100 ug | EUR 327.00 |
|
Description: Rabbit polyclonal ICK antibody |
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ICK Antibody |
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| 1-CSB-PA010955GA01HU | Cusabio |
|
|
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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ICK Antibody |
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| 1-CSB-PA008266 | Cusabio |
|
|
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
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ICK Antibody |
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| 1-CSB-PA008268 | Cusabio |
|
|
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
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 antibody) Anti-IGFBP7 (aa145-159) antibody |
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| STJ71903 | St John's Laboratory | 100 µg | EUR 359.00 |
 antibody) Anti-ACACB (aa146-159) antibody |
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| STJ72668 | St John's Laboratory | 100 µg | EUR 260.00 |
 Antibody) Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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| 20-abx006039 | Abbexa |
|
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|
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Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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| 20-abx012914 | Abbexa |
|
|
|
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Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
|||
| abx234108-100ug | Abbexa | 100 ug | EUR 509.00 |
|
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 Antibody) Serine/Threonine Protein Kinase ICK (ICK) Antibody |
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| abx331322-100ul | Abbexa | 100 ul | EUR 425.00 |
|
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Serine/Threonine Protein Kinase ICK (ICK) Antibody |
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| 20-abx326726 | Abbexa |
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|
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Serine/Threonine Protein Kinase ICK (ICK) Antibody |
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| 20-abx328497 | Abbexa |
|
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|
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Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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| 20-abx316647 | Abbexa |
|
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|
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) ICK antibody (Tyr159) |
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| 70R-32651 | Fitzgerald | 100 ug | EUR 327.00 |
|
Description: Rabbit polyclonal ICK antibody (Tyr159) |
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 ICK Polyclonal Antibody |
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| ABP54264-003ml | Abbkine | 0.03ml | EUR 158.00 |
|
|||
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Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290 |
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ICK Polyclonal Antibody |
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| ABP54264-01ml | Abbkine | 0.1ml | EUR 289.00 |
|
|||
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Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290 |
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ICK Polyclonal Antibody |
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| ABP54264-02ml | Abbkine | 0.2ml | EUR 414.00 |
|
|||
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Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290 |
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ICK Polyclonal Antibody |
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| ES5263-100ul | ELK Biotech | 100ul | EUR 279.00 |
|
Description: A Rabbit Polyclonal antibody against ICK from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA |
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ICK Polyclonal Antibody |
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| ES5263-50ul | ELK Biotech | 50ul | EUR 207.00 |
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Description: A Rabbit Polyclonal antibody against ICK from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA |
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 Ick/ Rat Ick ELISA Kit |
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| ELI-13214r | Lifescience Market | 96 Tests | EUR 886.00 |
 Antibody (HRP)) Serine/Threonine-Protein Kinase ICK (ICK) Antibody (HRP) |
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| 20-abx316648 | Abbexa |
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|
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 Antibody (FITC)) Serine/Threonine-Protein Kinase ICK (ICK) Antibody (FITC) |
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| 20-abx316649 | Abbexa |
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|
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 Antibody (Biotin)) Serine/Threonine-Protein Kinase ICK (ICK) Antibody (Biotin) |
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| 20-abx316650 | Abbexa |
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|
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 Cyanine 5 bisacid [equivalent to Cy5® bisacid] |
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| 159 | AAT Bioquest | 5 mg | EUR 219.00 |
|
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 ICK siRNA |
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| 20-abx902598 | Abbexa |
|
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|
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ICK siRNA |
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| 20-abx920119 | Abbexa |
|
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|
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ICK siRNA |
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| 20-abx920120 | Abbexa |
|
|
|
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 Antibody) ICK (Phospho-Tyr159) Antibody |
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| 12825-100ul | SAB | 100ul | EUR 252.00 |
ICK (Phospho-Tyr159) Antibody |
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| 12825-50ul | SAB | 50ul | EUR 187.00 |
 ICK Antibody, HRP conjugated |
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| 1-CSB-PA891800LB01HU | Cusabio |
|
|
|
|||
|
Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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 ICK Antibody, FITC conjugated |
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| 1-CSB-PA891800LC01HU | Cusabio |
|
|
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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 ICK Antibody, Biotin conjugated |
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| 1-CSB-PA891800LD01HU | Cusabio |
|
|
|
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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 Antibody) Phospho-ICK (Tyr159) Antibody |
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| DF8739 | Affbiotech | 200ul | EUR 304.00 |
|
Description: Phospho-ICK (Tyr159) Antibody detects endogenous levels of ICK only when phosphorylated at Tyr159. |
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Phospho-ICK (Tyr159) Antibody |
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| 20-abx216127 | Abbexa |
|
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|
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 Antibody) Phospho-ICK (Y159) Antibody |
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| 1-CSB-PA008265 | Cusabio |
|
|
|
|||
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Description: A polyclonal antibody against Phospho-ICK (Y159). Recognizes Phospho-ICK (Y159) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000 |
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 Antibody) Phospho- ICK (Tyr159) Antibody |
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| ABD8739 | Lifescience Market | 100 ug | EUR 438.00 |
 Anti-Braf |
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| DB-159-0.1 | DB Biotech | 100 μl | EUR 404.00 |
|
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Braf |
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| DB-159-0.2 | DB Biotech | 200 μl | EUR 587.00 |
|
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Braf |
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| DB-159-0.5 | DB Biotech | 500 μl | EUR 769.00 |
|
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Braf |
|||
| DB-159-1 | DB Biotech | 1 ml | EUR 1255.00 |
|
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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 ETC-159 |
|||
| HY-18988 | MedChemExpress | 10mM/1mL | EUR 113.00 |
 CV-159 |
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| HY-19025 | MedChemExpress | 1mg | EUR 849.00 |
 Polyclonal Goat anti-GST α-form |
|||
| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280.00 |
 Polyclonal Goat anti-GST μ-form |
|||
| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280.00 |
 Polyclonal Goat anti-GST p-form |
|||
| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280.00 |
 ELISA Kit) Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit |
|||
| abx595304-96tests | Abbexa | 96 tests | EUR 637.00 |
|
|||
 Anti-Ikaros Purified |
|||
| 11-159-C025 | ExBio | 0.025 mg | EUR 99.00 |
Anti-Ikaros Purified |
|||
| 11-159-C100 | ExBio | 0.1 mg | EUR 158.00 |
 Anti-Ikaros PE |
|||
| 1P-159-T025 | ExBio | 25 tests | EUR 140.00 |
Anti-Ikaros PE |
|||
| 1P-159-T100 | ExBio | 100 tests | EUR 240.00 |
 ICK cloning plasmid |
|||
| CSB-CL891800HU-10ug | Cusabio | 10ug | EUR 233.00 |
|
|||
|
Description: A cloning plasmid for the ICK gene. |
|||
 ICK Rabbit pAb |
|||
| A7783-100ul | Abclonal | 100 ul | EUR 308.00 |
ICK Rabbit pAb |
|||
| A7783-200ul | Abclonal | 200 ul | EUR 459.00 |
ICK Rabbit pAb |
|||
| A7783-20ul | Abclonal | 20 ul | EUR 183.00 |
ICK Rabbit pAb |
|||
| A7783-50ul | Abclonal | 50 ul | EUR 223.00 |
 Antibody) Intestinal Cell Kinase (ICK) Antibody |
|||
| 20-abx113263 | Abbexa |
|
|
|
|||
Intestinal Cell Kinase (ICK) Antibody |
|||
| 20-abx129209 | Abbexa |
|
|
|
|||
Intestinal Cell Kinase (ICK) Antibody |
|||
| 20-abx129873 | Abbexa |
|
|
|
|||
Intestinal Cell Kinase (ICK) Antibody |
|||
| 20-abx130408 | Abbexa |
|
|
|
|||
Intestinal Cell Kinase (ICK) Antibody |
|||
| abx331660-100ul | Abbexa | 100 ul | EUR 425.00 |
|
|||
 Mouse Serine/threonine- protein kinase ICK, Ick ELISA KIT |
|||
| ELI-43366m | Lifescience Market | 96 Tests | EUR 865.00 |
 ELISA Kit) Rat Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit |
|||
| abx391500-96tests | Abbexa | 96 tests | EUR 911.00 |
|
|||
 ELISA Kit) Human Serine/Threonine-Protein Kinase ICK (ICK) ELISA Kit |
|||
| abx387926-96tests | Abbexa | 96 tests | EUR 911.00 |
|
|||
 ELISA Kit) Mouse Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit |
|||
| abx389647-96tests | Abbexa | 96 tests | EUR 911.00 |
|
|||
 Human Serine/threonine- protein kinase ICK, ICK ELISA KIT |
|||
| ELI-48312h | Lifescience Market | 96 Tests | EUR 824.00 |
 Antibody) Anti-APLF (Ab-116) Antibody |
|||
| A06828 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal APLF (Ab-116) Antibody. Validated in IHC, WB and tested in Human. |
|||
 Antibody) Anti-Kir5.1 (Ab-416) Antibody |
|||
| A10858 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal Kir5.1 (Ab-416) Antibody. Validated in IF, IHC, WB and tested in Human. |
|||
 Antibody) Anti-GABRB1(Ab-434) Antibody |
|||
| A04752 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal GABRB1(Ab-434) Antibody. Validated in WB and tested in Human, Mouse, Rat. |
|||
 Antibody) Anti-MSK2 (Ab-568) Antibody |
|||
| A05545 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal MSK2 (Ab-568) Antibody. Validated in WB and tested in Human, Mouse. |
|||
 Antibody) Anti-JunD (Ab-255) Antibody |
|||
| A05609-2 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal JunD (Ab-255) Antibody. Validated in IF, IHC, WB and tested in Human. |
|||
 Antibody) Anti-CPI17a(Ab-38) Antibody |
|||
| A05752 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal CPI17a(Ab-38) Antibody. Validated in IF and tested in Human, Mouse, Rat. |
|||
 Antibody) Anti-Smad1 (Ab-465) Antibody |
|||
| A00728-3 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal Smad1 (Ab-465) Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
|||
 Antibody) Anti-GSK3b (Ab-9) Antibody |
|||
| A00791-1 | BosterBio | 100ul | EUR 397.00 |
|
Description: Rabbit Polyclonal GSK3b (Ab-9) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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