Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospital
Objectives: To assess the diagnostic performance of rapid lateral flow immunochromatographic assays (LFAs) compared with an ELISA and nucleic acid amplification tests (NATs) in individuals with suspected coronavirus disease 2019 (COVID-19).
Methods: Patients presenting to a Dutch teaching hospital were eligible between 17 March and 10 April 2020, when they had respiratory symptoms that were suspected for COVID-19. The performances of six different LFAs were evaluated in plasma samples obtained on corresponding respiratory sample dates of NATs testing. Subsequently, the best performing LFA was evaluated in 228 patients and in 50 sera of a historical patient control group.
Results: In the pilot analysis, sensitivity characteristics of LFA were heterogeneous, ranging from 2/20 (10%; 95% CI 0%-23%) to 11/20 (55%; 95% CI 33%-77%). In the total cohort, Orient Gene Biotech COVID-19 IgG/IgM Rapid Test LFA had a sensitivity of 43/99 (43%; 95% CI 34%-53%) and specificity of 126/129 (98%; 95% CI 95%-100%).
Sensitivity increased to 31/52 (60%; 95% CI 46%-73%) in patients with at least 7 days of symptoms, and to 21/33 (64%; 95% CI 47%-80%) in patients with C-reactive protein (CRP) ≥100 mg/L. Sensitivity and specificity of Wantai SARS-CoV-2 Ab ELISA was 59/95 (62%; 95% CI 52%-72%) and 125/128 (98%; 95% CI 95%-100%) in all patients, respectively, but sensitivity increased to 38/48 (79%; 95% CI 68%-91%) in patients with at least 7 days of symptoms.
Conclusions: There is large variability in diagnostic test performance between rapid LFAs, but overall limited sensitivity and high specificity in acutely admitted patients. Sensitivity improved in patients with longer existing symptoms or high CRP. LFAs should only be considered as additional triage tools when these may lead to the improvement of hospital logistics.

![]() Frit Kit |
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FRIT-KIT | Next Advance | 1each | EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
![]() Column Packing Kit |
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PACK-KIT | Next Advance | 1pack | EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
![]() PCR Mycoplasma Detection Kit |
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M034-Kit | TOKU-E | Kit | EUR 266 |
![]() Adiponectin (human) Elisa Assay Kit |
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K2192-96 | ApexBio | 96 assays | EUR 1017 |
![]() Adiponectin (mouse) Elisa Assay Kit |
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K2193-96 | ApexBio | 96 assays | EUR 1017 |
![]() Adiponectin (human) ELISA Assay Kit |
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K4901-100 | Biovision | EUR 898 |
![]() Adiponectin (mouse) Elisa Assay Kit |
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K4902-100 | Biovision | EUR 865 |
![]() Adiponectin (rat) Elisa Assay Kit |
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K4903-100 | Biovision | EUR 843 |
![]() Visfatin (human) Elisa Assay Kit |
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K4907-100 | Biovision | EUR 985 |
![]() Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
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![]() CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV100PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV105PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV125PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
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![]() Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
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![]() Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
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![]() T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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CAS510A-KIT | SBI | 1 Kit | EUR 805 |
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![]() Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV200PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
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![]() Histamine Assay Kit |
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AKR-360 | Cell Biolabs | 96 assays | EUR 519 |
Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve. |
![]() Glucose Assay Kit |
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abx090673-1Kit | Abbexa | 1 Kit | EUR 237 |
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![]() ADA Assay Kit |
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abx090675-100tests | Abbexa | 100 tests | EUR 237 |
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![]() Glutamate Assay Kit |
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abx096004-100Assays | Abbexa | 100 Assays | EUR 441 |
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![]() Glutathione Assay Kit |
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abx096005-100Assays | Abbexa | 100 Assays | EUR 378 |
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![]() Trehalase Assay Kit |
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abx096014-100Assays | Abbexa | 100 Assays | EUR 551 |
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![]() Pyruvate Assay Kit |
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abx097982-100Assays | Abbexa | 100 Assays | EUR 472 |
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![]() NADPase Assay Kit |
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abx097983-100Assays | Abbexa | 100 Assays | EUR 504 |
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![]() Starch Assay Kit |
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abx097988-100Assays | Abbexa | 100 Assays | EUR 441 |
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![]() Trehalose Assay Kit |
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abx097995-100Assays | Abbexa | 100 Assays | EUR 472 |
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![]() ADA Assay Kit |
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abx098403-Hitachi7060R190ml2R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 90ml×1 | EUR 739 |
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![]() ADA Assay Kit |
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abx098403-Hitachi7170R140ml4R220ml4 | Abbexa | Hitachi 7170; R1: 40ml×4 R2: 20ml×4 | EUR 801 |
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![]() ADA Assay Kit |
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abx098403-Hitachi7170R160ml4R260ml2 | Abbexa | Hitachi 7170; R1: 60ml×4 R2: 60ml×2 | EUR 911 |
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![]() ADA Assay Kit |
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abx098403-Toshiba40R150ml4R250ml2 | Abbexa | Toshiba 40; R1: 50ml×4 R2: 50ml×2 | EUR 786 |
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![]() Calcium Assay Kit |
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abx098414-Hitachi7020R140ml2R240ml2 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
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![]() Calcium Assay Kit |
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abx098414-Hitachi7060R190ml1R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×1 R2: 90ml×1 | EUR 206 |
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![]() Calcium Assay Kit |
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abx098414-Toshiba40R140ml2R240ml2 | Abbexa | Toshiba 40; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
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![]() Calcium Assay Kit |
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abx098414-UniversalR140ml2R240ml2 | Abbexa | Universal; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
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![]() Cholinesterase Assay Kit |
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abx098416-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 300 |
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![]() Cholinesterase Assay Kit |
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abx098416-Hitachi7170R140ml3R230ml1 | Abbexa | Hitachi 7170; R1: 40ml×3 R2: 30ml×1 | EUR 316 |
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![]() Cholinesterase Assay Kit |
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abx098416-Hitachi7170R160ml2R230ml1 | Abbexa | Hitachi 7170; R1: 60ml×2 R2: 30ml×1 | EUR 316 |
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![]() Cholinesterase Assay Kit |
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abx098416-Toshiba40R140ml4R240ml1 | Abbexa | Toshiba 40; R1: 40ml×4 R2: 40ml×1 | EUR 331 |
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![]() Cholinesterase Assay Kit |
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abx098416-UniversalR160ml2R215ml2 | Abbexa | Universal; R1: 60ml×2 R2: 15ml×2 | EUR 316 |
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![]() Creatinine Assay Kit |
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abx098422-Hitachi7020R150ml3R250ml1 | Abbexa | Hitachi 7020; R1: 50ml×3 R2: 50ml×1 | EUR 519 |
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![]() Creatinine Assay Kit |
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abx098422-Hitachi7060R190ml2R260ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 60ml×1 | EUR 472 |
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![]() Creatinine Assay Kit |
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abx098422-Toshiba120R140ml3R240ml1 | Abbexa | Toshiba 120; R1: 40ml×3 R2: 40ml×1 | EUR 566 |
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![]() Creatinine Assay Kit |
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abx098422-Toshiba120R150ml3R250ml1 | Abbexa | Toshiba 120; R1: 50ml×3 R2: 50ml×1 | EUR 519 |
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![]() Creatinine Assay Kit |
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abx098422-Toshiba40R150ml3R250ml1 | Abbexa | Toshiba 40; R1: 50ml×3 R2: 50ml×1 | EUR 519 |
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![]() Fructosamine Assay Kit |
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abx098427-Hitachi7020R140ml2R220ml1 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 20ml×1 | EUR 253 |
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![]() Fructosamine Assay Kit |
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abx098427-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 253 |
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![]() Fructosamine Assay Kit |
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abx098427-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 206 |
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![]() Fructosamine Assay Kit |
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abx098427-Hitachi7170R140ml12R240ml3 | Abbexa | Hitachi 7170; R1: 40ml×12 R2: 40ml×3 | EUR 222 |
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![]() Fructosamine Assay Kit |
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abx098427-Hitachi7170R140ml2R220ml1 | Abbexa | Hitachi 7170; R1: 40ml×2 R2: 20ml×1 | EUR 253 |
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![]() Glucose Assay Kit |
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abx098429-Hitachi7020R140ml4R240ml1 | Abbexa | Hitachi 7020; R1: 40ml×4 R2: 40ml×1 | EUR 222 |
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![]() Glucose Assay Kit |
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abx098429-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 222 |
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![]() Glucose Assay Kit |
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abx098429-Hitachi7170R132ml4R28ml4 | Abbexa | Hitachi 7170; R1: 32ml×4 R2: 8ml×4 | EUR 222 |
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![]() Haptoglobin Assay Kit |
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abx098432-BeckmanR140ml1R210ml1 | Abbexa | Beckman; R1: 40ml×1 R2: 10ml×1 | EUR 347 |
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![]() Haptoglobin Assay Kit |
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abx098432-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1: 40ml×1 R2: 10ml×1 | EUR 347 |
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![]() Haptoglobin Assay Kit |
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abx098432-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 269 |
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![]() Haptoglobin Assay Kit |
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abx098432-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1: 40ml×1 R2: 10ml×1 | EUR 347 |
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![]() Homocysteine Assay Kit |
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abx098434-Hitachi7060R160ml1R210ml1 | Abbexa | Hitachi 7060; R1: 60ml×1 R2: 10ml×1 | EUR 1036 |
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![]() Homocysteine Assay Kit |
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abx098434-Hitachi7170R130ml2R210ml1 | Abbexa | Hitachi 7170; R1: 30ml×2 R2: 10ml×1 | EUR 1036 |
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![]() Homocysteine Assay Kit |
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abx098434-Toshiba40R130ml2R210ml1 | Abbexa | Toshiba 40; R1: 30ml×2 R2: 10ml×1 | EUR 1036 |
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![]() Homocysteine Assay Kit |
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abx098434-UniversalR130ml2R210ml1 | Abbexa | Universal; R1: 30ml×2 R2: 10ml×1 | EUR 1036 |
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![]() Iron Assay Kit |
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abx098439-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1:40ml×1 R2:10ml×1 | EUR 206 |
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![]() Iron Assay Kit |
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abx098439-Hitachi7170R140ml1R210ml1 | Abbexa | Hitachi 7170; R1:40ml×1 R2:10ml×1 | EUR 206 |
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![]() Iron Assay Kit |
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abx098439-Hitachi7170R160ml1R215ml1 | Abbexa | Hitachi 7170; R1:60ml×1 R2:15ml×1 | EUR 222 |
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![]() Iron Assay Kit |
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abx098439-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1:40ml×1 R2:10ml×1 | EUR 206 |
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![]() Lipase Assay Kit |
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abx098442-BeckmanR180ml1R220ml1 | Abbexa | Beckman; R1:80ml×1 R2:20ml×1 | EUR 692 |
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![]() Lipase Assay Kit |
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abx098442-Hitachi7170R140ml2R220ml1 | Abbexa | Hitachi 7170; R1:40ml×2 R2:20ml×1 | EUR 692 |
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![]() Lipase Assay Kit |
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abx098442-Hitachi7170R160ml1R215ml1 | Abbexa | Hitachi 7170; R1:60ml×1 R2:15ml×1 | EUR 566 |
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![]() Lipase Assay Kit |
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abx098442-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1:40ml×1 R2:10ml×1 | EUR 441 |
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![]() Lipase Assay Kit |
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abx098442-UniversalR120ml1R25ml1 | Abbexa | Universal; R1:20ml×1 R2:5ml×1 | EUR 316 |
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![]() Magnesium Assay Kit |
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abx098445-Hitachi7020R140ml2R240ml2 | Abbexa | Hitachi 7020; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
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![]() Magnesium Assay Kit |
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abx098445-Hitachi7060R190ml1R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×1 R2: 90ml×1 | EUR 206 |
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![]() Magnesium Assay Kit |
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abx098445-Toshiba40R140ml2R240ml2 | Abbexa | Toshiba 40; R1: 40ml×2 R2: 40ml×2 | EUR 206 |
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![]() Magnesium Assay Kit |
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abx098445-Toshiba40R150ml2R250ml2 | Abbexa | Toshiba 40; R1: 50ml×2 R2: 50ml×2 | EUR 206 |
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![]() Microalbumin Assay KIt |
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abx098446-Hitachi7060R150ml1R210ml1 | Abbexa | Hitachi 7060; R1: 50ml×1 R2: 10ml×1 | EUR 363 |
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![]() Microalbumin Assay KIt |
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abx098446-Hitachi7060R150ml3R230ml1 | Abbexa | Hitachi 7060; R1: 50ml×3 R2: 30ml×1 | EUR 363 |
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![]() Microalbumin Assay KIt |
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abx098446-Hitachi7170R125ml1R25ml1 | Abbexa | Hitachi 7170; R1: 25ml×1 R2: 5ml×1 | EUR 269 |
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![]() Microalbumin Assay KIt |
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abx098446-Hitachi7170R150ml1R210ml1 | Abbexa | Hitachi 7170; R1: 50ml×1 R2: 10ml×1 | EUR 363 |
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![]() Myoglobin Assay Kit |
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abx098448-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1: 40ml×1 R2: 10ml×1 | EUR 1193 |
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![]() Myoglobin Assay Kit |
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abx098448-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 692 |
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![]() Myoglobin Assay Kit |
|||
abx098448-Hitachi7170R140ml1R210ml1 | Abbexa | Hitachi 7170; R1: 40ml×1 R2: 10ml×1 | EUR 1193 |
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![]() Myoglobin Assay Kit |
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abx098448-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1: 40ml×1 R2: 10ml×1 | EUR 1193 |
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![]() NGAL Assay Kit |
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abx098449-Hitachi7020R140ml1R210ml1 | Abbexa | Hitachi 7020; R1: 40ml×1 R2: 10ml×1 | EUR 942 |
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![]() NGAL Assay Kit |
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abx098449-Hitachi7170R120ml1R25ml1 | Abbexa | Hitachi 7170; R1: 20ml×1 R2: 5ml×1 | EUR 566 |
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![]() NGAL Assay Kit |
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abx098449-Hitachi7170R140ml1R210ml1 | Abbexa | Hitachi 7170; R1: 40ml×1 R2: 10ml×1 | EUR 942 |
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![]() NGAL Assay Kit |
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abx098449-Toshiba120R140ml1R210ml1 | Abbexa | Toshiba 120; R1: 40ml×1 R2: 10ml×1 | EUR 942 |
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![]() NGAL Assay Kit |
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abx098449-Toshiba40R140ml1R210ml1 | Abbexa | Toshiba 40; R1: 40ml×1 R2: 10ml×1 | EUR 942 |
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![]() Prealbumin Assay Kit |
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abx098451-BeckmanR140ml1R210ml1 | Abbexa | Beckman; R1: 40ml×1 R2: 10ml×1 | EUR 222 |
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![]() Prealbumin Assay Kit |
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abx098451-Hitachi7170R132ml1R28ml1 | Abbexa | Hitachi 7170; R1: 32ml×1 R2: 8ml×1 | EUR 222 |
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![]() Prealbumin Assay Kit |
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abx098451-UniversalR160ml1R215ml1 | Abbexa | Universal; R1: 60ml×1 R2: 15ml×1 | EUR 253 |
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![]() Transferrin Assay Kit |
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abx098460-BeckmanR160ml1R220ml1 | Abbexa | Beckman; R1: 60ml×1 R2: 20ml×1 | EUR 660 |
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![]() Transferrin Assay Kit |
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abx098460-Hitachi7060R160ml2R240ml1 | Abbexa | Hitachi 7060; R1: 60ml×2 R2: 40ml×1 | EUR 504 |
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![]() Transferrin Assay Kit |
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abx098460-Hitachi7170R145ml2R230ml1 | Abbexa | Hitachi 7170; R1: 45ml×2 R2: 30ml×1 | EUR 582 |
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![]() Transferrin Assay Kit |
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abx098460-Hitachi7170R160ml8R240ml4 | Abbexa | Hitachi 7170; R1: 60ml×8 R2: 40ml×4 | EUR 535 |
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![]() Transferrin Assay Kit |
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abx098460-Toshiba40R145ml2R230ml1 | Abbexa | Toshiba 40; R1: 45ml×2 R2: 30ml×1 | EUR 582 |
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![]() Triglycerides Assay Kit |
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abx098461-Hitachi7020R140ml4R240ml1 | Abbexa | Hitachi 7020; R1: 40ml×4 R2: 40ml×1 | EUR 284 |
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![]() Triglycerides Assay Kit |
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abx098461-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 269 |
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![]() Triglycerides Assay Kit |
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abx098461-Hitachi7170R132ml4R28ml4 | Abbexa | Hitachi 7170; R1: 32ml×4 R2: 8ml×4 | EUR 300 |
|
![]() Triglycerides Assay Kit |
|||
abx098461-Toshiba40R140ml4R240ml1 | Abbexa | Toshiba 40; R1: 40ml×4 R2: 40ml×1 | EUR 284 |
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![]() Urea Assay Kit |
|||
abx098464-Hitachi7020R150ml4R250ml1 | Abbexa | Hitachi 7020; R1: 50ml×4 R2: 50ml×1 | EUR 222 |
|
![]() Urea Assay Kit |
|||
abx098464-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 222 |
|
![]() Urea Assay Kit |
|||
abx098464-Toshiba120R140ml4R240ml1 | Abbexa | Toshiba 120; R1: 40ml×4 R2: 40ml×1 | EUR 222 |
|
![]() AMBP Assay Kit |
|||
abx098467-Hitachi7060R145ml1R215ml1 | Abbexa | Hitachi 7060; R1: 45ml×1 R2: 15ml×1 | EUR 488 |
|
![]() AMBP Assay Kit |
|||
abx098467-Hitachi7060R190ml2R230ml2 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 30ml×2 | EUR 519 |
|
![]() AMBP Assay Kit |
|||
abx098467-Hitachi7170R145ml1R215ml1 | Abbexa | Hitachi 7170; R1: 45ml×1 R2: 15ml×1 | EUR 488 |
|
![]() AMBP Assay Kit |
|||
abx098467-Toshiba40R145ml1R215ml1 | Abbexa | Toshiba 40; R1: 45ml×1 R2: 15ml×1 | EUR 488 |
|
![]() Catalase Assay Kit |
|||
abx294001-96tests | Abbexa | 96 tests | EUR 324 |
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![]() Hexokinase Assay Kit |
|||
abx294009-100tubes96samples | Abbexa | 100 tubes/96 samples | EUR 519 |
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![]() Ceruloplasmin Assay Kit |
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abx298825-100Assays | Abbexa | 100 Assays | EUR 394 |
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Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients
Objectives: To evaluate the diagnostic performance of seven rapid IgG/IgM tests and the Euroimmun IgA/IgG ELISA for antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in COVID-19 patients.
Methods: Specificity was evaluated in 103 samples collected before January 2020. Sensitivity and time to seropositivity was evaluated in 167 samples from 94 patients with COVID-19 confirmed with RT-PCR on nasopharyngeal swab.
Results: Specificity (confidence interval) of lateral flow assays (LFAs) was ≥91.3% (84.0-95.5) for IgM, ≥90.3% (82.9-94.8) for IgG, and ≥85.4% (77.2-91.1) for the combination IgM OR IgG. Specificity of the ELISA was 96.1% (90.1-98.8) for IgG and only 73.8% (64.5-81.4) for IgA. Sensitivity 14-25 days after the onset of symptoms was between ≥92.1% (78.5-98.0) and 100% (95.7-100) for IgG LFA compared to 89.5% (75.3-96.4) for IgG ELISA. Positivity of IgM OR IgG for LFA resulted in a decrease in specificity compared to IgG alone without a gain in diagnostic performance, except for VivaDiag.
The results for IgM varied significantly between the LFAs with an average overall agreement of only 70% compared to 89% for IgG. The average dynamic trend to seropositivity for IgM was not shorter than for IgG. At the time of hospital admission the sensitivity of LFA was <60%.
Conclusions: Sensitivity for the detection of IgG antibodies 14-25 days after the onset of symptoms was ≥92.1% for all seven LFAs compared to 89.5% for the IgG ELISA. The results for IgM varied significantly, and including IgM antibodies in addition to IgG for the interpretation of LFAs did not improve the diagnostic performance.
Association of Genetic Polymorphisms Related to Johne’s Disease With Estimated Breeding Values of Holstein Sires for Milk ELISATest Scores
Background: Johne’s disease (JD) is a chronic intestinal inflammatory disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection in ruminants. Since there are currently no effective vaccine or treatment options available to control JD, genetic selection may be an alternative strategy to enhance JD resistance.
Numerous Single Nucleotide Polymorphisms (SNPs) have been reported to be associated with MAP infection status based on published genome-wide association and candidate gene studies. The main objective of this study was to validate these SNPs that were previously identified to be associated with JD by testing their effect on Holstein bulls’ estimated breeding values (EBVs) for milk ELISA test scores, an indirect indicator of MAP infection status in cattle.
Results: Three SNPs, rs41810662, rs41617133 and rs110225854, located on Bos taurus autosomes (BTA) 16, 23 and 26, respectively, were confirmed as significantly associated with Holstein bulls’ EBVs for milk ELISA test score (FDR < 0.01) based on General Quasi Likelihood Scoring analysis (GQLS) analysis. Single-SNP regression analysis identified four SNPs that were associated with sire EBVs (FDR < 0.05).
This includes two SNPs that were common with GQLS (rs41810662 and rs41617133), with the other two SNPs being rs110494981 and rs136182707, located on BTA9 and BTA16, respectively.
Conclusions: The findings of this study validate the association of SNPs with JD MAP infection status and highlight the need to further investigate the genomic regions harboring these SNPs.
![]() ICK (Ab-159) Antibody |
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CSB-PA153658-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ICK (Ab-159). Recognizes ICK (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
![]() ICK (Ab-159) Conjugated Antibody |
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C33228 | SAB | 100ul | EUR 397 |
![]() Anti-GAB2 (Ab-159) Antibody |
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A02386-2 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal GAB2 (Ab-159) Antibody. Validated in IHC, WB and tested in Human. |
![]() MAK (Ab-159) Antibody |
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33233-100ul | SAB | 100ul | EUR 252 |
![]() MAK (Ab-159) Antibody |
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33233-50ul | SAB | 50ul | EUR 187 |
![]() GAB2 (Ab-159) Antibody |
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33262-100ul | SAB | 100ul | EUR 252 |
![]() GAB2 (Ab-159) Antibody |
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33262-50ul | SAB | 50ul | EUR 187 |
![]() GAB2 (Ab-159) Antibody |
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CSB-PA781252- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against GAB2 (Ab-159). Recognizes GAB2 (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
![]() GAB2 (Ab-159) Antibody |
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CSB-PA781252-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against GAB2 (Ab-159). Recognizes GAB2 (Ab-159) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
![]() MAK (Ab-159) Antibody |
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CSB-PA056397- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against MAK (Ab-159). Recognizes MAK (Ab-159) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
![]() MAK (Ab-159) Antibody |
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CSB-PA056397-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against MAK (Ab-159). Recognizes MAK (Ab-159) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
![]() MAK (Ab-159) Conjugated Antibody |
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C33233 | SAB | 100ul | EUR 397 |
![]() GAB2 (Ab-159) Conjugated Antibody |
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C33262 | SAB | 100ul | EUR 397 |
![]() Anti-ICK Antibody |
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A06270 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal ICK Antibody. Validated in IF, IHC and tested in Human, Mouse. |
![]() Anti-ICK Antibody |
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A1172-100 | Biovision | EUR 370 |
![]() Anti-ICK antibody |
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PAab04108 | Lifescience Market | 100 ug | EUR 386 |
![]() Anti-ICK antibody |
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STJ93636 | St John's Laboratory | 200 µl | EUR 197 |
Description: Rabbit polyclonal to ICK. |
![]() Anti-ICK antibody |
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STJ110093 | St John's Laboratory | 100 µl | EUR 277 |
Description: Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. |
![]() anti-ICK |
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YF-PA17639 | Abfrontier | 100 ug | EUR 403 |
Description: Rabbit polyclonal to ICK |
![]() anti-ICK |
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YF-PA25803 | Abfrontier | 50 ul | EUR 334 |
Description: Mouse polyclonal to ICK |
![]() Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
![]() ICK antibody |
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70R-32652 | Fitzgerald | 100 ug | EUR 327 |
Description: Rabbit polyclonal ICK antibody |
![]() ICK antibody |
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70R-32214 | Fitzgerald | 100 ug | EUR 327 |
Description: Rabbit polyclonal ICK antibody |
![]() ICK Antibody |
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34122-100ul | SAB | 100ul | EUR 252 |
![]() ICK Antibody |
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34122-50ul | SAB | 50ul | EUR 187 |
![]() ICK Antibody |
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42899-100ul | SAB | 100ul | EUR 252 |
![]() ICK antibody |
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70R-17871 | Fitzgerald | 50 ul | EUR 435 |
Description: Rabbit polyclonal ICK antibody |
![]() ICK Antibody |
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1-CSB-PA891800LA01HU | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
![]() ICK Antibody |
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1-CSB-PA008266 | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
![]() ICK Antibody |
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1-CSB-PA008268 | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
![]() ICK Antibody |
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1-CSB-PA010955GA01HU | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
![]() ICK Antibody |
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CSB-PA278117- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500 |
![]() ICK Antibody |
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CSB-PA278117-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500 |
![]() Anti-ACACB (aa146-159) antibody |
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STJ72668 | St John's Laboratory | 100 µg | EUR 260 |
![]() Anti-IGFBP7 (aa145-159) antibody |
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STJ71903 | St John's Laboratory | 100 µg | EUR 359 |
![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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20-abx012914 | Abbexa |
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![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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20-abx006039 | Abbexa |
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![]() Serine/Threonine Protein Kinase ICK (ICK) Antibody |
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20-abx326726 | Abbexa |
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![]() Serine/Threonine Protein Kinase ICK (ICK) Antibody |
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20-abx328497 | Abbexa |
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![]() Serine/Threonine Protein Kinase ICK (ICK) Antibody |
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abx331322-100ul | Abbexa | 100 ul | EUR 425 |
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![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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20-abx316647 | Abbexa |
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![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody |
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abx234108-100ug | Abbexa | 100 ug | EUR 509 |
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![]() ICK Polyclonal Antibody |
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ES5263-100ul | ELK Biotech | 100ul | EUR 279 |
Description: A Rabbit Polyclonal antibody against ICK from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA |
![]() ICK Polyclonal Antibody |
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ES5263-50ul | ELK Biotech | 50ul | EUR 207 |
Description: A Rabbit Polyclonal antibody against ICK from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA |
![]() ICK Polyclonal Antibody |
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ABP54264-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290 |
![]() ICK Polyclonal Antibody |
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ABP54264-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290 |
![]() ICK Polyclonal Antibody |
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ABP54264-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290 |
![]() ICK antibody (Tyr159) |
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70R-32651 | Fitzgerald | 100 ug | EUR 327 |
Description: Rabbit polyclonal ICK antibody (Tyr159) |
![]() Ick/ Rat Ick ELISA Kit |
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ELI-13214r | Lifescience Market | 96 Tests | EUR 886 |
![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody (HRP) |
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20-abx316648 | Abbexa |
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![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody (FITC) |
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20-abx316649 | Abbexa |
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![]() Serine/Threonine-Protein Kinase ICK (ICK) Antibody (Biotin) |
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20-abx316650 | Abbexa |
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![]() Cyanine 5 bisacid [equivalent to Cy5® bisacid] |
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159 | AAT Bioquest | 5 mg | EUR 219 |
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![]() ICK siRNA |
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20-abx902598 | Abbexa |
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![]() ICK siRNA |
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20-abx920119 | Abbexa |
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![]() ICK siRNA |
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20-abx920120 | Abbexa |
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![]() Phospho- ICK (Tyr159) Antibody |
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ABD8739 | Lifescience Market | 100 ug | EUR 438 |
![]() Phospho-ICK (Tyr159) Antibody |
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20-abx216127 | Abbexa |
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![]() ICK (Phospho-Tyr159) Antibody |
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12825-100ul | SAB | 100ul | EUR 252 |
![]() ICK (Phospho-Tyr159) Antibody |
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12825-50ul | SAB | 50ul | EUR 187 |
![]() Phospho-ICK (Tyr159) Antibody |
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DF8739 | Affbiotech | 200ul | EUR 304 |
Description: Phospho-ICK (Tyr159) Antibody detects endogenous levels of ICK only when phosphorylated at Tyr159. |
![]() ICK Antibody, HRP conjugated |
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1-CSB-PA891800LB01HU | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
![]() ICK Antibody, FITC conjugated |
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1-CSB-PA891800LC01HU | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
![]() ICK Antibody, Biotin conjugated |
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1-CSB-PA891800LD01HU | Cusabio |
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
![]() Phospho-ICK (Y159) Antibody |
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1-CSB-PA008265 | Cusabio |
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Description: A polyclonal antibody against Phospho-ICK (Y159). Recognizes Phospho-ICK (Y159) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000 |
![]() Anti-Braf |
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DB-159-0.1 | DB Biotech | 100 μl | EUR 404 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
![]() Anti-Braf |
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DB-159-0.2 | DB Biotech | 200 μl | EUR 587 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
![]() Anti-Braf |
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DB-159-0.5 | DB Biotech | 500 μl | EUR 769 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
![]() Anti-Braf |
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DB-159-1 | DB Biotech | 1 ml | EUR 1255 |
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
![]() ETC-159 |
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HY-18988 | MedChemExpress | 10mM/1mL | EUR 113 |
![]() CV-159 |
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HY-19025 | MedChemExpress | 1mg | EUR 849 |
![]() Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
![]() Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
![]() Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
![]() Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit |
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abx595304-96tests | Abbexa | 96 tests | EUR 637 |
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![]() Anti-Ikaros Purified |
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11-159-C025 | ExBio | 0.025 mg | EUR 99 |
![]() Anti-Ikaros Purified |
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11-159-C100 | ExBio | 0.1 mg | EUR 158 |
![]() Anti-Ikaros PE |
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1P-159-T025 | ExBio | 25 tests | EUR 140 |
![]() Anti-Ikaros PE |
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1P-159-T100 | ExBio | 100 tests | EUR 240 |
![]() ICK Rabbit pAb |
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A7783-100ul | Abclonal | 100 ul | EUR 308 |
![]() ICK Rabbit pAb |
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A7783-200ul | Abclonal | 200 ul | EUR 459 |
![]() ICK Rabbit pAb |
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A7783-20ul | Abclonal | 20 ul | EUR 183 |
![]() ICK Rabbit pAb |
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A7783-50ul | Abclonal | 50 ul | EUR 223 |
![]() ICK cloning plasmid |
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CSB-CL891800HU-10ug | Cusabio | 10ug | EUR 233 |
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Description: A cloning plasmid for the ICK gene. |
![]() Intestinal Cell Kinase (ICK) Antibody |
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20-abx113263 | Abbexa |
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![]() Intestinal Cell Kinase (ICK) Antibody |
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20-abx129209 | Abbexa |
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![]() Intestinal Cell Kinase (ICK) Antibody |
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20-abx129873 | Abbexa |
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![]() Intestinal Cell Kinase (ICK) Antibody |
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20-abx130408 | Abbexa |
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![]() Intestinal Cell Kinase (ICK) Antibody |
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abx331660-100ul | Abbexa | 100 ul | EUR 425 |
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![]() Anti-BRCA1 (Ab-1524) Antibody |
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A00005-1 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal BRCA1 (Ab-1524) Antibody. Validated in IF, WB and tested in Human. |
![]() Anti-Bcr (Ab-177) Antibody |
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A00022-1 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal Bcr (Ab-177) Antibody. Validated in IF, WB and tested in Human, Mouse. |
![]() Anti-JAK2 (Ab-570) Antibody |
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A00027 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal JAK2 (Ab-570) Antibody. Validated in IF, WB and tested in Human, Mouse. |
![]() Anti-Jak2 (Ab-570) Antibody |
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A00027-2 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal Jak2 (Ab-570) Antibody. Validated in IHC and tested in Human, Rat. |
![]() Anti-MDM2 (Ab-166) Antibody |
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A00054-1 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal MDM2 (Ab-166) Antibody. Validated in IF, IHC, WB and tested in Human. |
![]() Anti-eIF4E (Ab-209) Antibody |
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A00135 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal eIF4E (Ab-209) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
![]() Anti-p27Kip1 (Ab-10) Antibody |
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A00173 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal p27Kip1 (Ab-10) Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
![]() Anti-FLT3(Ab-591) Antibody |
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A00188 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal FLT3(Ab-591) Antibody. Validated in IF, WB and tested in Human. |
![]() Anti-CDC2 (Ab-15) Antibody |
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A00209 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal CDC2 (Ab-15) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
![]() Anti-VASP (Ab-157) Antibody |
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A00303 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal VASP (Ab-157) Antibody. Validated in IF, WB and tested in Human, Rat. |
![]() Anti-JAK1 (Ab-1022) Antibody |
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A00330-1 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal JAK1 (Ab-1022) Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
![]() Anti-CDK6 (Ab-13) Antibody |
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A00358-1 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal CDK6 (Ab-13) Antibody. Validated in IF, IHC and tested in Human, Mouse. |
![]() Anti-GATA1 (Ab-142) Antibody |
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A00408-1 | BosterBio | 100ul | EUR 397 |
Description: Rabbit Polyclonal GATA1 (Ab-142) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |