Comparison of diagnostic accuracies of rapid serological tests and ELISA

Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospital

Objectives: To assess the diagnostic performance of rapid lateral flow immunochromatographic assays (LFAs) compared with an ELISA and nucleic acid amplification tests (NATs) in individuals with suspected coronavirus disease 2019 (COVID-19).
Methods: Patients presenting to a Dutch teaching hospital were eligible between 17 March and 10 April 2020, when they had respiratory symptoms that were suspected for COVID-19. The performances of six different LFAs were evaluated in plasma samples obtained on corresponding respiratory sample dates of NATs testing. Subsequently, the best performing LFA was evaluated in 228 patients and in 50 sera of a historical patient control group.
Results: In the pilot analysis, sensitivity characteristics of LFA were heterogeneous, ranging from 2/20 (10%; 95% CI 0%-23%) to 11/20 (55%; 95% CI 33%-77%). In the total cohort, Orient Gene Biotech COVID-19 IgG/IgM Rapid Test LFA had a sensitivity of 43/99 (43%; 95% CI 34%-53%) and specificity of 126/129 (98%; 95% CI 95%-100%).
Sensitivity increased to 31/52 (60%; 95% CI 46%-73%) in patients with at least 7 days of symptoms, and to 21/33 (64%; 95% CI 47%-80%) in patients with C-reactive protein (CRP) ≥100 mg/L. Sensitivity and specificity of Wantai SARS-CoV-2 Ab ELISA was 59/95 (62%; 95% CI 52%-72%) and 125/128 (98%; 95% CI 95%-100%) in all patients, respectively, but sensitivity increased to 38/48 (79%; 95% CI 68%-91%) in patients with at least 7 days of symptoms.
Conclusions: There is large variability in diagnostic test performance between rapid LFAs, but overall limited sensitivity and high specificity in acutely admitted patients. Sensitivity improved in patients with longer existing symptoms or high CRP. LFAs should only be considered as additional triage tools when these may lead to the improvement of hospital logistics.
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MELISA-TEK® High Sensitivity Extraction Kit

510391 96 Well Ask for price

Meat Speciation : EZ Pangasius™

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540EZPKR 10 Tests Ask for price

Visfatin (human) Elisa Assay Kit

GWB-AXR242 100 assays Ask for price

Visfatin (human) Elisa Assay Kit

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Adiponectin (rat) Elisa Assay Kit

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Adiponectin (rat) Elisa Assay Kit

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Adiponectin (human) Elisa Assay Kit

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Adiponectin (mouse) Elisa Assay Kit

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Adiponectin (human) Elisa Assay Kit

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Description: Tools Kit|ELISA Kit#Kits|Tools Kit#Kits

Adiponectin (human) ELISA Assay Kit

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Adiponectin (mouse) Elisa Assay Kit

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Adiponectin (mouse) Elisa Assay Kit

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Ultra Nuclease Assay Kit(ELISA)

HBP000111 96 Tests
EUR 609.5

Feline SAA Detection ELISA Assay Kit

6028 1 kit
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Description: Liquid Samples

Elite cAMP ELISA Assay Kit (Fluorescence)

MBS433536-1Plate 1Plate
EUR 605

Elite cAMP ELISA Assay Kit (Fluorescence)

MBS433536-5x1Plate 5x1Plate
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Ultra Nuclease Assay Kit 2.0(ELISA)

HBP000116 96 Tests
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Cas9 ELISA Assay

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Cas9 ELISA Assay

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K2/Spice Synthetic Cannabinoids ELISA Assay Kit

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OVALBUMIN SPECIFIC IgE ELISA ASSAY KIT (OKSA11256)

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EUR 4191.6
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Screen Questâ„¢ Colorimetric ELISA cAMP Assay Kit

36370-1plate 1 plate
EUR 334
Description: Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction.

Screen Questâ„¢ Colorimetric ELISA cAMP Assay Kit

36371-10plates 10 plates
EUR 2197
Description: Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction.

Screen Questâ„¢ Fluorimetric ELISA cAMP Assay Kit

36373-1plate 1 plate
EUR 334
Description: Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems.

Screen Questâ„¢ Fluorimetric ELISA cAMP Assay Kit

36374-10plates 10 plates
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Description: Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems.

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SL1999Hu 96 Tests
EUR 468

Elite cAMP 384-Well ELISA Assay Kit (Fluorescence)

MBS433543-1Plate 1Plate
EUR 845

Elite cAMP 384-Well ELISA Assay Kit (Fluorescence)

MBS433543-5x1Plate 5x1Plate
EUR 3900

Elite gp120 (HIV-1/Clade B) ELISA Assay Kit

MBS433541-200Assays 200Assays
EUR 730

Elite gp120 (HIV-1/Clade B) ELISA Assay Kit

MBS433541-5x200Assays 5x200Assays
EUR 3355

Rat uPA total antigen assay ELISA Kit

MBS135495-1Kit 1Kit
EUR 750

Rat uPA total antigen assay ELISA Kit

MBS135495-5x1Kit 5x1Kit
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Rat tPA total antigen assay ELISA Kit

MBS135505-1Kit 1Kit
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Rat tPA total antigen assay ELISA Kit

MBS135505-5x1Kit 5x1Kit
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ELISA Assay Buffer, 20x

GR103001 1 L
EUR 89

ELISA Assay Buffer, 80x

GR103002 1 L
EUR 159

Mouse uPA total antigen assay ELISA Kit

MBS135504-1Kit 1Kit
EUR 750

Mouse uPA total antigen assay ELISA Kit

MBS135504-5x1Kit 5x1Kit
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Human tPA total antigen assay ELISA Kit

MBS135510-1Kit 1Kit
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Human tPA total antigen assay ELISA Kit

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Human uPA total antigen assay ELISA Kit

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Human uPA total antigen assay ELISA Kit

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Mouse tPA total antigen assay ELISA Kit

MBS135528-1Kit 1Kit
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Mouse tPA total antigen assay ELISA Kit

MBS135528-5x1Kit 5x1Kit
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Active rat tPA functional assay ELISA Kit

MBS135493-1Kit 1Kit
EUR 750

Active rat tPA functional assay ELISA Kit

MBS135493-5x1Kit 5x1Kit
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Active rat uPA functional assay ELISA Kit

MBS135544-1Kit 1Kit
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Active rat uPA functional assay ELISA Kit

MBS135544-5x1Kit 5x1Kit
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Rat Fibrinogen ELISA assay

MBS480412-1Kit 1Kit
EUR 625

Rat Fibrinogen ELISA assay

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Creatinine Assay Kit (urine normalizing assay)

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EUR 220

Creatinine Assay Kit (urine normalizing assay)

MBS480387-5x1Kit 5x1Kit
EUR 960

Active human uPA functional assay ELISA Kit

MBS135509-1Kit 1Kit
EUR 750

Active human uPA functional assay ELISA Kit

MBS135509-5x1Kit 5x1Kit
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Active mouse uPA functional assay ELISA Kit

MBS135514-1Kit 1Kit
EUR 750

Active mouse uPA functional assay ELISA Kit

MBS135514-5x1Kit 5x1Kit
EUR 3420

Active human tPA functional assay ELISA Kit

MBS135524-1Kit 1Kit
EUR 750

Active human tPA functional assay ELISA Kit

MBS135524-5x1Kit 5x1Kit
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Active mouse tPA functional assay ELISA Kit

MBS135539-1Kit 1Kit
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Active mouse tPA functional assay ELISA Kit

MBS135539-5x1Kit 5x1Kit
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MBS433548-200Assays 200Assays
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Hemagglutinin (H1N1)(A/California/07/09) ELISA Assay Kit

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EUR 3355

Active rabbit tPA functional assay ELISA Kit

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Rat fibrinogen total antigen assay ELISA Kit

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Rat fibrinogen total antigen assay ELISA Kit

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EUR 2220

Mouse Fibrinogen ELISA assay

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Mouse Fibrinogen ELISA assay

MBS480411-5x1Kit 5x1Kit
EUR 3000

Active porcine tPA functional assay ELISA Kit

MBS135507-1Kit 1Kit
EUR 750

Active porcine tPA functional assay ELISA Kit

MBS135507-5x1Kit 5x1Kit
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Human Kininogen total antigen assay ELISA kit

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Human Kininogen total antigen assay ELISA kit

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MBS376015-5x96Wells 5x96Wells
EUR 1145

Mouse fibrinogen total antigen assay ELISA Kit

MBS135511-1Kit 1Kit
EUR 490

Mouse fibrinogen total antigen assay ELISA Kit

MBS135511-5x1Kit 5x1Kit
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Human fibrinogen total antigen assay ELISA Kit

MBS135523-1Kit 1Kit
EUR 475

Human fibrinogen total antigen assay ELISA Kit

MBS135523-5x1Kit 5x1Kit
EUR 2120

Rat PAI-1 total antigen assay ELISA Kit

MBS135541-1Kit 1Kit
EUR 725

Rat PAI-1 total antigen assay ELISA Kit

MBS135541-5x1Kit 5x1Kit
EUR 3300

Human antiplasmin total antigen assay ELISA kit

MBS135932-1Kit 1Kit
EUR 750

Human antiplasmin total antigen assay ELISA kit

MBS135932-5x1Kit 5x1Kit
EUR 3420

Mouse antiplasmin total antigen assay ELISA kit

MBS135940-1Kit 1Kit
EUR 750

Mouse antiplasmin total antigen assay ELISA kit

MBS135940-5x1Kit 5x1Kit
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Human plasminogen total antigen assay ELISA Kit

MBS135487-1Kit 1Kit
EUR 490

Human plasminogen total antigen assay ELISA Kit

MBS135487-5x1Kit 5x1Kit
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Mouse plasminogen total antigen assay ELISA Kit

MBS135494-1Kit 1Kit
EUR 580

Mouse plasminogen total antigen assay ELISA Kit

MBS135494-5x1Kit 5x1Kit
EUR 2630

Human Prothrombin total antigen assay ELISA Kit

MBS135499-1Kit 1Kit
EUR 490

Human Prothrombin total antigen assay ELISA Kit

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MBS135501-1Kit 1Kit
EUR 750

Rabbit fibrinogen total antigen assay ELISA Kit

MBS135501-5x1Kit 5x1Kit
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Mouse Prothrombin total antigen assay ELISA Kit

MBS135518-1Kit 1Kit
EUR 520

Mouse Prothrombin total antigen assay ELISA Kit

MBS135518-5x1Kit 5x1Kit
EUR 2340

Human vitronectin total antigen assay ELISA Kit

MBS135522-1Kit 1Kit
EUR 750

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MBS135522-5x1Kit 5x1Kit
EUR 3420

Mouse vitronectin total antigen assay ELISA Kit

MBS135526-1Kit 1Kit
EUR 750

Mouse vitronectin total antigen assay ELISA Kit

MBS135526-5x1Kit 5x1Kit
EUR 3420

Mouse IL-2 total antigen assay ELISA kit

MBS136013-1Kit 1Kit
EUR 590

Mouse IL-2 total antigen assay ELISA kit

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EUR 2675

Porcine fibrinogen total antigen assay ELISA Kit

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Porcine fibrinogen total antigen assay ELISA Kit

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EpiQuik CRISPR/Cas9 Assay ELISA Kit (Colorimetric)

P-4060
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  • 96 Assays
  • 48 Assays
  • 96 Assays

ELISA GENERAL ASSAY DILUENT

MBS238291-100mL 100mL
EUR 210

ELISA GENERAL ASSAY DILUENT

MBS238291-5x100mL 5x100mL
EUR 770

ELISA NEPTUNE ASSAY DILUENT

MBS238293-100mL 100mL
EUR 235

ELISA NEPTUNE ASSAY DILUENT

MBS238293-5x100mL 5x100mL
EUR 885

ELISA GENERAL ASSAY DILUENT

MBS239226-500mL 500mL
EUR 535

ELISA GENERAL ASSAY DILUENT

MBS239226-5x500mL 5x500mL
EUR 2240

ELISA GENERAL ASSAY DILUENT

MBS239227-1L 1L
EUR 680

ELISA GENERAL ASSAY DILUENT

MBS239227-5x1L 5x1L
EUR 2930

ELISA NEPTUNE ASSAY DILUENT

MBS239230-500mL 500mL
EUR 525

ELISA NEPTUNE ASSAY DILUENT

MBS239230-5x500mL 5x500mL
EUR 2185

Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients

Methods: Specificity was evaluated in 103 samples collected before January 2020. Sensitivity and time to seropositivity was evaluated in 167 samples from 94 patients with COVID-19 confirmed with RT-PCR on nasopharyngeal swab.
Results: Specificity (confidence interval) of lateral flow assays (LFAs) was ≥91.3% (84.0-95.5) for IgM, ≥90.3% (82.9-94.8) for IgG, and ≥85.4% (77.2-91.1) for the combination IgM OR IgG. Specificity of the ELISA was 96.1% (90.1-98.8) for IgG and only 73.8% (64.5-81.4) for IgA. Sensitivity 14-25 days after the onset of symptoms was between ≥92.1% (78.5-98.0) and 100% (95.7-100) for IgG LFA compared to 89.5% (75.3-96.4) for IgG ELISA. Positivity of IgM OR IgG for LFA resulted in a decrease in specificity compared to IgG alone without a gain in diagnostic performance, except for VivaDiag.
The results for IgM varied significantly between the LFAs with an average overall agreement of only 70% compared to 89% for IgG. The average dynamic trend to seropositivity for IgM was not shorter than for IgG. At the time of hospital admission the sensitivity of LFA was <60%.
Conclusions: Sensitivity for the detection of IgG antibodies 14-25 days after the onset of symptoms was ≥92.1% for all seven LFAs compared to 89.5% for the IgG ELISA. The results for IgM varied significantly, and including IgM antibodies in addition to IgG for the interpretation of LFAs did not improve the diagnostic performance.

Association of Genetic Polymorphisms Related to Johne’s Disease With Estimated Breeding Values of Holstein Sires for Milk ELISATest Scores

Background: Johne’s disease (JD) is a chronic intestinal inflammatory disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection in ruminants. Since there are currently no effective vaccine or treatment options available to control JD, genetic selection may be an alternative strategy to enhance JD resistance.
Numerous Single Nucleotide Polymorphisms (SNPs) have been reported to be associated with MAP infection status based on published genome-wide association and candidate gene studies. The main objective of this study was to validate these SNPs that were previously identified to be associated with JD by testing their effect on Holstein bulls’ estimated breeding values (EBVs) for milk ELISA test scores, an indirect indicator of MAP infection status in cattle.
Results: Three SNPs, rs41810662, rs41617133 and rs110225854, located on Bos taurus autosomes (BTA) 16, 23 and 26, respectively, were confirmed as significantly associated with Holstein bulls’ EBVs for milk ELISA test score (FDR < 0.01) based on General Quasi Likelihood Scoring analysis (GQLS) analysis. Single-SNP regression analysis identified four SNPs that were associated with sire EBVs (FDR < 0.05).
This includes two SNPs that were common with GQLS (rs41810662 and rs41617133), with the other two SNPs being rs110494981 and rs136182707, located on BTA9 and BTA16, respectively.
Conclusions: The findings of this study validate the association of SNPs with JD MAP infection status and highlight the need to further investigate the genomic regions harboring these SNPs.

ICK (Ab-159) Antibody

E11-8112B 100μg
EUR 225
Description: Available in various conjugation types.

ICK (Ab-159) Antibody

E11-8122B 100μg
EUR 225
Description: Available in various conjugation types.

ICK (Ab-159) Antibody

MBS7132078-01mL 0.1mL
EUR 270

ICK (Ab-159) Antibody

MBS7132078-5x01mL 5x0.1mL
EUR 1200

ICK (Ab-159) Antibody

MBS851572-01mg 0.1mg
EUR 305

ICK (Ab-159) Antibody

MBS851572-01mLAF405L 0.1mL(AF405L)
EUR 465

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EUR 465

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EUR 465

ICK (Ab-159) Antibody

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ICK (Ab-159) Antibody

MBS859945-01mg 0.1mg
EUR 305

ICK (Ab-159) Antibody

MBS859945-01mLAF405L 0.1mL(AF405L)
EUR 465

ICK (Ab-159) Antibody

MBS859945-01mLAF405S 0.1mL(AF405S)
EUR 465

ICK (Ab-159) Antibody

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EUR 465

ICK (Ab-159) Antibody

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ICK (Ab-159) Antibody

MBS9409751-005mL 0.05mL
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ICK (Ab-159) Antibody

MBS9409751-01mL 0.1mL
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ICK (Ab-159) Antibody

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ICK (Ab-159) Conjugated Antibody

C33228 100ul
EUR 476.4

ICK (Ab-159) Conjugated Antibody

MBS9444252-01mLAF350 0.1mL(AF350)
EUR 480

ICK (Ab-159) Conjugated Antibody

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ICK (Ab-159) Conjugated Antibody

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EUR 480

ICK (Ab-159) Conjugated Antibody

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EUR 480

ICK (Ab-159) Conjugated Antibody

MBS9444252-01mLBiotin 0.1mL(Biotin)
EUR 480

Rabbit polyclonal ICK (Ab-159) antibody

TA315191 100 µl Ask for price

Anti-DNA Antibody

12403 100ug
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Anti-DNA Antibody

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Anti-DNA Antibody

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EUR 903

Anti-DNA Antibody

12403-500 500ug
EUR 460

Anti-DNA Antibody

12404 100ug
EUR 230

Anti-DNA Antibody

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Anti-DNA Antibody

12404-1000 1mg
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Anti-DNA Antibody

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Anti-GAB2 (Ab-159) Antibody

A02386-2 100ul
EUR 360
Description: Boster Bio Anti-GAB2 (Ab-159) Antibody (Catalog # A02386-2). Tested in WB, IHC applications. This antibody reacts with Human.

Anti-Cytochrome P450 Antibody

13006 200ug
EUR 621

Anti-Cytochrome P450 Antibody

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Anti-Cytochrome P450 Antibody

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Anti-DNA, Intercalated, Antibody

12401 100ug
EUR 230

Anti-DNA, Intercalated, Antibody

12401-100 100ug
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Anti-DNA, Intercalated, Antibody

12401-1000 1mg
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Anti-DNA, Intercalated, Antibody

12401-500 500ug
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Anti-DNA, Intercalated, Antibody

12402 100ug
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Anti-DNA, Intercalated, Antibody

12402-100 100ug
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Anti-DNA, Intercalated, Antibody

12402-1000 1mg
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Anti-DNA, Intercalated, Antibody

12402-500 500ug
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Anti-Glutathione Reductase Antibody

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Anti-Glutathione Reductase Antibody

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Anti-DNA Ligase I Antibody

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Anti-DNA Ligase III Antibody

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Anti-Rotavirus Group Antigen Antibody

20601 100ug
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Anti-RNA Polymerase II Antibody

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Anti-Heme Oxygenase-1 Antibody

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Anti-Heme Oxygenase-1 Antibody

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Anti-Heme Oxygenase-2 Antibody

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Anti-Myelin Basic Protein Antibody

24201 500ug
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Anti-V5 Antibody, Agarose immobilized

18868A 100ug
EUR 496

Anti-Superoxide dismutase 1 Antibody

13064 100ug
EUR 457

Anti-Superoxide dismutase 1 Antibody

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Anti-ECS Antibody, Agarose immobilized

18830A 100ug
EUR 506

Anti-Acrolein Mouse Monoclonal Antibody

56606 100 ug
EUR 441

Anti-Cytochrome P450 Reductase Antibody

13000-50 50ug
EUR 364

Anti-Citrulline Mouse Monoclonal Antibody

56601 100 ug
EUR 441

Anti-Citrulline Mouse Monoclonal Antibody

56602 100 ug
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Anti-Dityrosine Mouse Monoclonal Antibody

56621 100 ug
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Anti-Dityrosine Mouse Monoclonal Antibody

56622 100 ug
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Anti-Angiotensin Converting Enzyme Antibody

19501 100ug
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Anti-Methylglyoxal Mouse Monoclonal Antibody

56617 100 ug
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Anti-Methylglyoxal Mouse Monoclonal Antibody

56618 100 ug
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Anti-Sulfotyrosine Mouse Monoclonal Antibody

56623 100 ug
EUR 441

Anti-Human IgG Fc specific Antibody

87003 1mg
EUR 215

Anti-Factor V Light Chain Antibody

19701 250ug
EUR 538

Anti-Mouse IgG Fc specific Antibody

83001 1mg
EUR 210

Anti-Malondialdehyde Mouse Monoclonal Antibody

56615 100 ug
EUR 441

Anti-Malondialdehyde Mouse Monoclonal Antibody

56616 100 ug
EUR 441

Anti-Matallothionein Mouse Monoclonal Antibody

56628 100 ug
EUR 357

Anti-Matallothionein Mouse Monoclonal Antibody

56629 100 ug
EUR 357

Anti-Matallothionein Mouse Monoclonal Antibody

56630 100 ug
EUR 357

Anti-Rabbit IgG Fc specific Antibody

84001 1mg
EUR 219

Anti-Factor XII Light Chain Antibody

19802 250ug
EUR 538

Anti-Rat Slo2.2 Mouse Monoclonal Antibody

56632 100 ug
EUR 357

Anti-Sphingosine Kinase type 1 Antibody

13810 50ug
EUR 454

Anti-c-myc Antibody, Agarose immobilized

18824A 100ug
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TSH (beta) | thyroid stimulating hormone (Affinity purified, F(ab)2' fragment, goat antibody)

AS10-1325 0,5 mg
EUR 532

Anti-4-Hydroxynonenal Mouse Monoclonal Antibody

56612 100 ug
EUR 441

Anti-Protein Disulfide Isomerase (PDI) Antibody

11081 100ul
EUR 473

Anti-7-Ketocholesterol Mouse Monoclonal Antibody

56614 100 ug
EUR 441

Anti-6-Nitrotryptophan Mouse Monoclonal Antibody

56619 100 ug
EUR 441

Anti-6-Nitrotryptophan Mouse Monoclonal Antibody

56620 100 ug
EUR 441

Anti-Glutathione-S-Transferase (GST) Antibody

18803 100ug
EUR 503

Anti-Glutathione-S-Transferase (GST) Antibody

18842-01 100ug
EUR 271

Anti-Glutathione-S-Transferase (GST) Antibody

18844 1mg
EUR 1647

Anti-Glutathione-S-Transferase (GST) Antibody

18844-01 100ug
EUR 271

Anti-Retinoblastoma protein phosphoserine 612 Antibody

3113 25ug
EUR 200

Anti-Retinoblastoma protein phosphoserine 780 Antibody

3114 25ug
EUR 200

Anti-Retinoblastoma protein phosphoserine 811 Antibody

3116 25ug
EUR 200

Anti-KBA.62 (Melanoma Associated Antigen) Antibody

34062 100ul
EUR 250

Anti-Dibromo-tyrosine Mouse Monoclonal Antibody

56607 100 ug
EUR 441

Anti-Dibromo-tyrosine Mouse Monoclonal Antibody

56608 100 ug
EUR 441

Anti-Retinoblastoma protein phosphothreonine 821 Antibody

3117 25ug
EUR 200

Anti-Retinoblastoma protein phosphothreonine 826 Antibody

3118 25ug
EUR 200

Goat anti-Mouse IgG Fc, F(ab)´2 Fragment

AS16-3485 1,0 mg
EUR 206

Anti-Entamoeba histolytica Mouse Monoclonal Antibody

150101 5 x 100ug
EUR 664