Comparison of diagnostic accuracies of rapid serological tests and ELISA

September 8, 2020

Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospital

Objectives: To assess the diagnostic performance of rapid lateral flow immunochromatographic assays (LFAs) compared with an ELISA and nucleic acid amplification tests (NATs) in individuals with suspected coronavirus disease 2019 (COVID-19).
Methods: Patients presenting to a Dutch teaching hospital were eligible between 17 March and 10 April 2020, when they had respiratory symptoms that were suspected for COVID-19. The performances of six different LFAs were evaluated in plasma samples obtained on corresponding respiratory sample dates of NATs testing. Subsequently, the best performing LFA was evaluated in 228 patients and in 50 sera of a historical patient control group.
Results: In the pilot analysis, sensitivity characteristics of LFA were heterogeneous, ranging from 2/20 (10%; 95% CI 0%-23%) to 11/20 (55%; 95% CI 33%-77%). In the total cohort, Orient Gene Biotech COVID-19 IgG/IgM Rapid Test LFA had a sensitivity of 43/99 (43%; 95% CI 34%-53%) and specificity of 126/129 (98%; 95% CI 95%-100%).
Sensitivity increased to 31/52 (60%; 95% CI 46%-73%) in patients with at least 7 days of symptoms, and to 21/33 (64%; 95% CI 47%-80%) in patients with C-reactive protein (CRP) ≥100 mg/L. Sensitivity and specificity of Wantai SARS-CoV-2 Ab ELISA was 59/95 (62%; 95% CI 52%-72%) and 125/128 (98%; 95% CI 95%-100%) in all patients, respectively, but sensitivity increased to 38/48 (79%; 95% CI 68%-91%) in patients with at least 7 days of symptoms.
Conclusions: There is large variability in diagnostic test performance between rapid LFAs, but overall limited sensitivity and high specificity in acutely admitted patients. Sensitivity improved in patients with longer existing symptoms or high CRP. LFAs should only be considered as additional triage tools when these may lead to the improvement of hospital logistics.
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DPP4 Assay Kit
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Diagnostic performance of seven rapid IgG/IgM antibody tests and the Euroimmun IgA/IgG ELISA in COVID-19 patients

Objectives: To evaluate the diagnostic performance of seven rapid IgG/IgM tests and the Euroimmun IgA/IgG ELISA for antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in COVID-19 patients.
Methods: Specificity was evaluated in 103 samples collected before January 2020. Sensitivity and time to seropositivity was evaluated in 167 samples from 94 patients with COVID-19 confirmed with RT-PCR on nasopharyngeal swab.
Results: Specificity (confidence interval) of lateral flow assays (LFAs) was ≥91.3% (84.0-95.5) for IgM, ≥90.3% (82.9-94.8) for IgG, and ≥85.4% (77.2-91.1) for the combination IgM OR IgG. Specificity of the ELISA was 96.1% (90.1-98.8) for IgG and only 73.8% (64.5-81.4) for IgA. Sensitivity 14-25 days after the onset of symptoms was between ≥92.1% (78.5-98.0) and 100% (95.7-100) for IgG LFA compared to 89.5% (75.3-96.4) for IgG ELISA. Positivity of IgM OR IgG for LFA resulted in a decrease in specificity compared to IgG alone without a gain in diagnostic performance, except for VivaDiag.
The results for IgM varied significantly between the LFAs with an average overall agreement of only 70% compared to 89% for IgG. The average dynamic trend to seropositivity for IgM was not shorter than for IgG. At the time of hospital admission the sensitivity of LFA was <60%.
Conclusions: Sensitivity for the detection of IgG antibodies 14-25 days after the onset of symptoms was ≥92.1% for all seven LFAs compared to 89.5% for the IgG ELISA. The results for IgM varied significantly, and including IgM antibodies in addition to IgG for the interpretation of LFAs did not improve the diagnostic performance.

Association of Genetic Polymorphisms Related to Johne’s Disease With Estimated Breeding Values of Holstein Sires for Milk ELISATest Scores

Background: Johne’s disease (JD) is a chronic intestinal inflammatory disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection in ruminants. Since there are currently no effective vaccine or treatment options available to control JD, genetic selection may be an alternative strategy to enhance JD resistance.
Numerous Single Nucleotide Polymorphisms (SNPs) have been reported to be associated with MAP infection status based on published genome-wide association and candidate gene studies. The main objective of this study was to validate these SNPs that were previously identified to be associated with JD by testing their effect on Holstein bulls’ estimated breeding values (EBVs) for milk ELISA test scores, an indirect indicator of MAP infection status in cattle.
Results: Three SNPs, rs41810662, rs41617133 and rs110225854, located on Bos taurus autosomes (BTA) 16, 23 and 26, respectively, were confirmed as significantly associated with Holstein bulls’ EBVs for milk ELISA test score (FDR < 0.01) based on General Quasi Likelihood Scoring analysis (GQLS) analysis. Single-SNP regression analysis identified four SNPs that were associated with sire EBVs (FDR < 0.05).
This includes two SNPs that were common with GQLS (rs41810662 and rs41617133), with the other two SNPs being rs110494981 and rs136182707, located on BTA9 and BTA16, respectively.
Conclusions: The findings of this study validate the association of SNPs with JD MAP infection status and highlight the need to further investigate the genomic regions harboring these SNPs.

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1-CSB-PA008266
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ICK Antibody
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ICK Antibody
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Description: A polyclonal antibody against ICK. Recognizes ICK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

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ICK Antibody
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Serine/Threonine-Protein Kinase ICK (ICK) Antibody
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  • 20 ug
  • 50 ug

Serine/Threonine Protein Kinase ICK (ICK) Antibody
20-abx328497
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Serine/Threonine Protein Kinase ICK (ICK) Antibody
20-abx326726
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Anti-IGFBP7 (aa145-159) antibody
STJ71903 100 µg
EUR 430.8

Anti-ACACB (aa146-159) antibody
STJ72668 100 µg
EUR 312

Ick/ Rat Ick ELISA Kit
ELI-13214r 96 Tests
EUR 1063.2

ICK Polyclonal Antibody
ABP54264-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290

ICK Polyclonal Antibody
ABP54264-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290

ICK Polyclonal Antibody
ABP54264-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of ICK from Human, Mouse, Rat. This ICK antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ICK at AA rangle: 210-290

ICK Polyclonal Antibody
ES5263-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against ICK from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA

ICK Polyclonal Antibody
ES5263-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against ICK from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA

ICK antibody (Tyr159)
70R-32651 100 ug
EUR 392.4
Description: Rabbit polyclonal ICK antibody (Tyr159)

Cyanine 5 bisacid [equivalent to Cy5® bisacid]
159 5 mg
EUR 262.8

Serine/Threonine-Protein Kinase ICK (ICK) Antibody (HRP)
20-abx316648
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Serine/Threonine-Protein Kinase ICK (ICK) Antibody (FITC)
20-abx316649
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Serine/Threonine-Protein Kinase ICK (ICK) Antibody (Biotin)
20-abx316650
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ICK siRNA
20-abx920119
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

ICK siRNA
20-abx920120
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

ICK siRNA
20-abx902598
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Phospho-ICK (Y159) Antibody
1-CSB-PA008265
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-ICK (Y159). Recognizes Phospho-ICK (Y159) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

Phospho-ICK (Tyr159) Antibody
DF8739 200ul
EUR 420

Phospho-ICK (Tyr159) Antibody
AF3816 100ul
EUR 540

ICK Antibody, HRP conjugated
1-CSB-PA891800LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ICK Antibody, FITC conjugated
1-CSB-PA891800LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ICK Antibody, Biotin conjugated
1-CSB-PA891800LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ICK. Recognizes ICK from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ICK (Phospho-Tyr159) Antibody
12825-100ul 100ul
EUR 302.4

ICK (Phospho-Tyr159) Antibody
12825-50ul 50ul
EUR 224.4

Phospho- ICK (Tyr159) Antibody
ABD8739 100 ug
EUR 525.6

Phospho-ICK (Tyr159) Antibody
20-abx216127
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 336

Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit
abx595304-96tests 96 tests
EUR 764.4

Anti-Braf
DB-159-0.1 100 μl
EUR 484.8
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Braf
DB-159-0.2 200 μl
EUR 704.4
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Braf
DB-159-0.5 500 μl
EUR 922.8
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Braf
DB-159-1 1 ml
EUR 1506
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

ICK Rabbit pAb
A7783-100ul 100 ul
EUR 369.6

ICK Rabbit pAb
A7783-200ul 200 ul
EUR 550.8

ICK Rabbit pAb
A7783-20ul 20 ul
EUR 219.6

ICK Rabbit pAb
A7783-50ul 50 ul
EUR 267.6

ICK cloning plasmid
CSB-CL891800HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the ICK gene.

ICK, GST-tag
40112 10 µg
EUR 410
Description: Recombinant human intestinal cell kinase (ICK), full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

ETC-159
HY-18988 10mM/1mL
EUR 135.6

CV-159
HY-19025 1mg
EUR 1018.8

Intestinal Cell Kinase (ICK) Antibody
20-abx129873
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Intestinal Cell Kinase (ICK) Antibody
20-abx130408
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Intestinal Cell Kinase (ICK) Antibody
20-abx113263
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Intestinal Cell Kinase (ICK) Antibody
20-abx129209
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Intestinal Cell Kinase (ICK) Antibody
abx331660-100ul 100 ul
EUR 510

Anti-Ikaros PE
1P-159-T025 25 tests
EUR 168

Anti-Ikaros PE
1P-159-T100 100 tests
EUR 288

Anti-Ikaros Purified
11-159-C025 0.025 mg
EUR 118.8

Anti-Ikaros Purified
11-159-C100 0.1 mg
EUR 189.6

Human Serine/Threonine-Protein Kinase ICK (ICK) ELISA Kit
abx387926-96tests 96 tests
EUR 1093.2

Rat Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit
abx391500-96tests 96 tests
EUR 1093.2

Mouse Serine/Threonine Protein Kinase ICK (ICK) ELISA Kit
abx389647-96tests 96 tests
EUR 1093.2

Mouse Serine/threonine- protein kinase ICK, Ick ELISA KIT
ELI-43366m 96 Tests
EUR 1038

Human Serine/threonine- protein kinase ICK, ICK ELISA KIT
ELI-48312h 96 Tests
EUR 988.8

Anti-APLF (Ab-116) Antibody
A06828 100ul
EUR 476.4
Description: Rabbit Polyclonal APLF (Ab-116) Antibody. Validated in IHC, WB and tested in Human.

Anti-Kir5.1 (Ab-416) Antibody
A10858 100ul
EUR 476.4
Description: Rabbit Polyclonal Kir5.1 (Ab-416) Antibody. Validated in IF, IHC, WB and tested in Human.

Anti-BIM(Ab-69) Antibody
A01552-1 100ul
EUR 476.4
Description: Rabbit Polyclonal BIM(Ab-69) Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-ADD1 (Ab-726) Antibody
A01575 100ul
EUR 476.4
Description: Rabbit Polyclonal ADD1 (Ab-726) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-eNOS (Ab-1177) Antibody
A01604-1 100ul
EUR 476.4
Description: Rabbit Polyclonal eNOS (Ab-1177) Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-TYK2(Ab-1054) Antibody
A01642 100ul
EUR 476.4
Description: Rabbit Polyclonal TYK2(Ab-1054) Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-PKCz(Ab-410) Antibody
A01796 100ul
EUR 476.4
Description: Rabbit Polyclonal PKCz(Ab-410) Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-NMDAR1 (Ab-897) Antibody
A01808-1 100ul
EUR 476.4
Description: Rabbit Polyclonal NMDAR1 (Ab-897) Antibody. Validated in IF and tested in Human, Mouse, Rat.